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IDH3A Antibody (OTI2F11) [DyLight 650]

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Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2F11
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 650

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IDH3A Antibody (OTI2F11) [DyLight 650] Summary

Immunogen
Full length recombinant protein of human IDH3A (NP_005521) produced in HEK293T cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IDH3A
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for IDH3A Antibody (OTI2F11) [DyLight 650]

  • EC 1.1.1
  • EC 1.1.1.41
  • H-IDH alpha
  • isocitrate dehydrogenase (NAD+) alpha chain
  • isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
  • isocitrate dehydrogenase 3 (NAD+) alpha
  • Isocitric dehydrogenase subunit alpha
  • NAD(+)-specific ICDH subunit alpha
  • NAD(H)-specific isocitrate dehydrogenase alpha subunit
  • NAD+-specific ICDH

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IDH3A Antibody (NBP2-70992C) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IDH3A