![Western Blot: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IBRDC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IBRDC2.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Western-Blot-NBP2-72470-img0001.jpg "Western Blot: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IBRDC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IBRDC2.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI9H10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RNF144B |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 33.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
![Western Blot PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Western_Blot_11082.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11144.jpg)
![Immunohistochemistry PAK3 Antibody [Unconjugated]](https://images.novusbio.com/images2/PAK3_AF6897_Immunohistochemistry_11480.jpg)
Secondary Antibodies |
Isotype Controls |
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The Latest Research on IBR-type E3 Ubiquitin Ligases E3 ubiquitin ligases are standards in most antibody catalogs. These proteins are essential to the process of ubiquitination, which is expressed in protein pathways throughout the body and is often linked to disease states. It is widely used as a bioma... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | RNF144B |
![Immunocytochemistry/Immunofluorescence: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IBRDC2.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-72470-img0005.jpg "Immunocytochemistry/Immunofluorescence: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IBRDC2.")
![Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human Ovary tissue using anti-IBRDC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72470-img0009.jpg "Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human Ovary tissue using anti-IBRDC2 mouse monoclonal antibody.")
![Flow Cytometry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of HeLa cells using anti-IBRDC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72470-img0004.jpg "Flow Cytometry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of HeLa cells using anti-IBRDC2 mouse monoclonal antibody.")
![Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human colon tissue using anti-IBRDC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72470-img0006.jpg "Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human colon tissue using anti-IBRDC2 mouse monoclonal antibody.")
![Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human endometrium tissue using anti-IBRDC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72470-img0007.jpg "Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human endometrium tissue using anti-IBRDC2 mouse monoclonal antibody.")
![Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human Kidney tissue using anti-IBRDC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72470-img0008.jpg "Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human Kidney tissue using anti-IBRDC2 mouse monoclonal antibody.")
![Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human prostate tissue using anti-IBRDC2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-72470-img0010.jpg "Immunohistochemistry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Staining of paraffin-embedded Human prostate tissue using anti-IBRDC2 mouse monoclonal antibody.")
![Flow Cytometry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Analysis of Jurkat cells, using anti-IBRDC2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72470-img0002.jpg "Flow Cytometry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - Analysis of Jurkat cells, using anti-IBRDC2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).")
![Flow Cytometry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IBRDC2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IBRDC2-Antibody-OTI9H10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72470-img0003.jpg "Flow Cytometry: IBRDC2 Antibody (OTI9H10) - Azide and BSA Free [NBP2-72470] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IBRDC2 antibody, and then analyzed by flow cytometry.")
