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Huntingtin Antibody (HDB4E10)

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Western Blot: Huntingtin Antibody (HDB4E10) [NB600-1197] - Western Blot analysis of normal human cerebral cortex total protein extract run on a 3-12.5% gradient SDS-PAGE gel and probed with Mouse anti Huntingtin

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
HDB4E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Huntingtin Antibody (HDB4E10) Summary

Immunogen
Recombinant protein corresponding to amino acids 1844 - 2131 of huntingtin
Localization
Cytoplasmic
Specificity
This reacts with an epitope corresponding to the HDB region (amino acids 1844 - 2131) of the huntingtin protein.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HTT
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Immunohistology on frozen (paraffin not tested). Western blotting detects a 350kD band on western blots but also detects smaller degradation products of huntingtin. This clone recognizes both denatured and native huntingtin in human brain.
Control
Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Predicted cross-reactivities: Human, Mouse, Rabbit

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Huntingtin Antibody (HDB4E10)

  • HD protein
  • HD
  • HTT
  • huntingtin (Huntington disease)
  • Huntingtin
  • IT15
  • IT15HDHuntington disease protein

Background

The combined use of clones HDC8A4, HDB4E10 and HDA3E10 demonstrate that huntingtin is enriched in neuronal cells in the brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Huntingtin Antibody (NB600-1197) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Huntingtin.

Mechanisms of Neurodegeneration: Protein aggregation and failure of autophagy
By Michalina Hanzel, PhDIn a series of three blog posts I will briefly explore the major cellular mechanisms responsible for many neurodegenerative disorders. The first, and perhaps the most apparent, is the accumulat...  Read full blog post.

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Bioinformatics

Gene Symbol HTT
Entrez
Uniprot