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Human TNF-alpha Quantikine ELISA Kit

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human TNF-alpha Quantikine ELISA Kit Summary

Background
The Quantikine Human TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against this protein. It has been shown to accurately quantitate the recombinant factor. Results obtained with naturally occurring TNF-alpha samples showe...d linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha .
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Specificity
Natural and recombinant human TNF-alpha .
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DTA00D in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TNF-alpha Quantikine ELISA Kit

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor Necrosis Factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer (5-11).  
Human TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (12, 13). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus monkey, and 71%-92% aa identity with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha . It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (14). Cell surface TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling following ligation by soluble TNF RI (15, 16). Shedding of membrane bound TNF-alpha by TACE/ ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (17-19).  
TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (20, 21) and the hematopoietic cell-restricted 78-80 kDa TNF RII (22, 23), both of which are also expressed as homotrimers (1, 24). Both type I and type II receptors bind TNF-alpha with comparable affinity and can promote NF kappa B activation (25-28). Only TNF RI, however, contains a cytoplasmic death domain which triggers the activation of apoptosis (3, 29). Soluble forms of both types of receptors are released into human serum and urine and can neutralize the biological activity of TNF-alpha (30-32).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for TNF-alpha (DTA00D)(539)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Primate, Primate - Cholrocebus pygerythrus (Vervet Monkey), Primate - Pan troglodytes (Chimpanzee), Rabbit.

We have publications tested in 3 applications: ELISA, ELISA (Standard), ELISA Capture.


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ELISA
(1)
ELISA (Standard)
(1)
ELISA Capture
(3)
All Applications
Filter By Species
Human
(527)
Mouse
(6)
Rat
(2)
Primate
(1)
Primate - Cholrocebus pygerythrus (Vervet Monkey)
(1)
Primate - Pan troglodytes (Chimpanzee)
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 539. Show All 539 Publications.
Publications using DTA00D Applications Species
Longo, M;Paolini, E;Di Benedetto, P;Tomassini, E;Meroni, M;Dongiovanni, P; DGAT1 and DGAT2 Inhibitors for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Management: Benefits for Their Single or Combined Application International journal of molecular sciences 2024-08-21 [PMID: 39201759] (Human) Human
Malek, E;Rana, PS;Swamydas, M;Daunov, M;Miyagi, M;Murphy, E;Ignatz-Hoover, JJ;Metheny, L;Kim, SJ;Driscoll, JJ; The TGF? type I receptor kinase inhibitor vactosertib in combination with pomalidomide in relapsed/refractory multiple myeloma: a phase 1b trial Nature communications 2024-08-27 [PMID: 39191755] (Human) Human
Donate-Correa, J;Ferri, CM;Mora-Fernández, C;Pérez-Delgado, N;González-Luis, A;Navarro-González, JF; Pentoxifylline ameliorates subclinical atherosclerosis progression in patients with type 2 diabetes and chronic kidney disease: a randomized pilot trial Cardiovascular diabetology 2024-08-24 [PMID: 39182114] (Human) Human
Han, L;Sun, X;Kong, J;Li, J;Feng, K;Bai, Y;Wang, X;Zhu, Z;Yang, F;Chen, Q;Zhang, M;Yue, B;Wang, X;Fu, L;Chen, Y;Yang, Q;Wang, S;Xin, Q;Sun, N;Zhang, D;Zhou, Y;Gao, Y;Zhao, J;Jiang, Y;Guo, R; Multi-omics analysis reveals a feedback loop amplifying immune responses in acute graft-versus-host disease due to imbalanced gut microbiota and bile acid metabolism Journal of translational medicine 2024-08-07 [PMID: 39113144] (Human) Human
Peng, Z;Zhang, W;Hong, H;Liu, L; Effect of luteolin on oxidative stress and inflammation in the human osteoblast cell line hFOB1.19 in an inflammatory microenvironment BMC pharmacology & toxicology 2024-07-12 [PMID: 38997762] (Human) Human
Kutuk, MO;Tufan, AE;Kilicaslan, F;Gokcen, C;Aksu, GG;Yektas, C;Kandemir, H;Celik, F;Mutluer, T;Buber, A;Karadag, M;Coban, N;Coskun, S;Hangul, Z;Altintas, E;Acikbas, U;Giray, A;Aka, Y;Basturk, B;Kutuk, O; Cytokine expression profiles in children and adolescents with tic disorders Scientific reports 2024-07-02 [PMID: 38956051] (Human) Human
Kim, H;Park, HH;Kim, HN;Seo, D;Hong, KS;Jang, JG;Seo, EU;Kim, IY;Jeon, SY;Son, B;Cho, SW;Kim, W;Ahn, JH;Lee, W; The TOX-RAGE axis mediates inflammatory activation and lung injury in severe pulmonary infectious diseases Proceedings of the National Academy of Sciences of the United States of America 2024-06-25 [PMID: 38900789] (Human) Human
Zhang, Y;Jiang, H;Dong, M;Min, J;He, X;Tan, Y;Liu, F;Chen, M;Chen, X;Yin, Q;Zheng, L;Shao, Y;Li, X;Chen, H; Macrophage MCT4 inhibition activates reparative genes and protects from atherosclerosis by histone H3 lysine 18 lactylation Cell reports 2024-05-28 [PMID: 38733581] (Human) Human
Kim, MJ;Ahn, HJ;Kong, D;Lee, S;Kim, DH;Kang, KS; Modeling of solar UV-induced photodamage on the hair follicles in human skin organoids Journal of tissue engineering 2024-05-08 [PMID: 38725732] (Human) Human
Melgar-Rodríguez, S;Polanco, A;Ríos-Muñoz, J;García, M;Sierra-Cristancho, A;González-Osuna, L;Díaz-Zúñiga, J;Carvajal, P;Vernal, R;Bravo, D; Differential Response of Human Dendritic Cells upon Stimulation with Encapsulated or Non-Encapsulated Isogenic Strains of Porphyromonas gingivalis International journal of molecular sciences 2024-04-20 [PMID: 38674095] (Human) Human
Show All 539 Publications.

Reviews for TNF-alpha (DTA00D) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF-alpha (DTA00D). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Additional TNF-alpha Products

Blogs on TNF-alpha. Showing 1-10 of 13 blog posts - Show all blog posts.

Unlocking the Potential of Biosimilars in Immuno-Oncology
By Jennifer Jones, M.S.Biosimilar Antibodies: Imitation Meets InnovationIn the ever-evolving medical landscape, a new class of pharmaceuticals is emerging as a game-changer, poised to transform the way we approach...  Read full blog post.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.


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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
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