I need to know about TNF-alpha antibody kits for mice.

We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.

I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.

I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.

I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?

The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.

Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?

I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Human TNF-alpha QuantiGlo ELISA Kit (QTA00C): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	ELISA
Conjugate	HRP

Background	The QuantiGlo Human TNF-alpha Immunoassay is a 5 hour solid phase chemiluminescent ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human TNF-alpha . Results ob...tained using naturally occurring TNF-alpha samples showed linear curves that were parallel to the standard curves obtained using the QuantiGlo^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha . Show More
Specificity	Natural and recombinant human TNF-alpha .
Source	N/A
Inter-Assay	See PDF Datasheet for details
Intra-Assay	See PDF Datasheet for details
Spike Recovery	See PDF Datasheet for details
Sample Volume	See PDF Datasheet for details

Publications using QTA00C	Applications	Species
Mucientes, A;Lisbona-Montañez, JM;Mena-Vázquez, N;Ruiz-Limón, P;Manrique-Arija, S;García-Studer, A;Ortiz-Márquez, F;Fernández-Nebro, A; Intestinal Dysbiosis, Tight Junction Proteins, and Inflammation in Rheumatoid Arthritis Patients: A Cross-Sectional Study International journal of molecular sciences 2024-08-08 [PMID: 39201334] (Human)		Human
Du, F;Rische, CH;Li, Y;Vincent, MP;Krier-Burris, RA;Qian, Y;Yuk, SA;Almunif, S;Bochner, BS;Qiao, B;Scott, EA; Controlled adsorption of multiple bioactive proteins enables targeted mast cell nanotherapy Nature nanotechnology 2024-01-16 [PMID: 38228804] (Human)		Human
Iwahori, K;Nii, T;Yamaguchi, N;Kawasaki, T;Okamura, S;Hashimoto, K;Matsuki, T;Tsujino, K;Miki, K;Osa, A;Goya, S;Abe, K;Mori, M;Takeda, Y;Yamada, T;Kida, H;Kumanogoh, A; A randomized phase 2 study on demeclocycline in patients with mild-to-moderate COVID-19 Scientific reports 2023-08-23 [PMID: 37612352] (Human)		Human
F Du, C Rische, Y Li, M Vincent, R Krier-Burr, Y Qian, S Yuk, S Almunif, B Bochner, B Qiao, E Scott Bioactive multi-protein adsorption enables targeted mast cell nanotherapy Research square, 2023-01-26;0(0):. 2023-01-26 [PMID: 36747749] (Human)		Human
I Bortone, R Zupo, F Castellana, S Aresta, L Lampignano, S Sciarra, C Griseta, TA Stallone, G Sborgia, M Lozupone, F Panza, G Lagravines, P Battista, R Sardone Motoric Cognitive Risk Syndrome, Subtypes and 8-Year All-Cause Mortality in Aging Phenotypes: The Salus in Apulia Study Brain sciences, 2022-06-29;12(7):. 2022-06-29 [PMID: 35884669] (Human)		Human
R Zupo, F Castellana, S De Nucci, G De Pergola, M Lozupone, I Bortone, M Castellana, G Sborgia, L Lampignano, G Giannelli, F Panza, R Sardone Liver Fibrosis and 8-Year All-Cause Mortality Trajectories in the Aging Cohort of the Salus in Apulia Study Biomedicines, 2021-11-04;9(11):. 2021-11-04 [PMID: 34829846] (Human)		Human
T Tamura, M Suzuki, K Hayashida, Y Kobayashi, J Yoshizawa, T Shibusawa, M Sano, S Hori, J Sasaki Hydrogen gas inhalation alleviates oxidative stress in patients with post-cardiac arrest syndrome J Clin Biochem Nutr, 2020-04-03;67(2):214-221. 2020-04-03 [PMID: 33041520] (Human)		Human
V Manganelli, A Capozzi, S Truglia, C Alessandri, E Lococo, T Garofalo, C De Carolis, F Conti, G Valesini, M Sorice, A Longo, R Misasi Elevated Serum Level of HMGB1 in Patients with the Antiphospholipid Syndrome J Immunol Res, 2017-12-19;2017(0):4570715. 2017-12-19 [PMID: 29410969] (Human)		Human
A Yoshihisa, Y Kanno, S Watanabe, T Yokokawa, S Abe, M Miyata, T Sato, S Suzuki, M Oikawa, A Kobayashi, T Yamaki, H Kunii, K Nakazato, H Suzuki, T Ishida, Y Takeishi Impact of nutritional indices on mortality in patients with heart failure Open Heart, 2018-01-09;5(1):e000730. 2018-01-09 [PMID: 29344381] (Human)		Human
M Minatoya, S Itoh, A Araki, N Tamura, K Yamazaki, C Miyashita, R Kishi Association between Fetal Adipokines and Child Behavioral Problems at Preschool Age: The Hokkaido Study on Environment and Children's Health Int J Environ Res Public Health, 2018-01-11;15(1):. 2018-01-11 [PMID: 29324697] (Human)		Human
HE Wajdner, J Farrington, C Barnard, PT Peachell, CG Schnackenb, JP Marino, X Xu, K Affleck, M Begg, EP Seward Orai and TRPC channel characterization in Fc?RI-mediated calcium signaling and mediator secretion in human mast cells Physiol Rep, 2017-03-01;5(5):. 2017-03-01 [PMID: 28292887] (Human)		Human
Kawamura T, Umemura T, Umegaki H, Imamine R, Kawano N, Tanaka C, Kawai M, Minatoguchi M, Kusama M, Kouchi Y, Watarai A, Kanai A, Nakashima E, Hotta N Effect of renal impairment on cognitive function during a 3-year follow up in elderly patients with type 2 diabetes: Association with microinflammation. J Diabetes Investig, 2014-02-04;5(5):597-605. 2014-02-04 [PMID: 25411629] (Human)		Human
Gao H, Mejhert N, Fretz J, Arner E, Lorente-Cebrian S, Ehrlund A, Dahlman-Wright K, Gong X, Stromblad S, Douagi I, Laurencikiene J, Dahlman I, Daub C, Ryden M, Horowitz M, Arner P Early B cell factor 1 regulates adipocyte morphology and lipolysis in white adipose tissue. Cell Metab, 2014-05-22;19(6):981-92. 2014-05-22 [PMID: 24856929] (Human)		Human
Cifuentes M, Fuentes C, Tobar N, Acevedo I, Villalobos E, Hugo E, Ben-Jonathan N, Reyes M Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose tissue. Mol Cell Endocrinol, 2012-03-16;361(1):24-30. 2012-03-16 [PMID: 22449852] (Human)		Human
Narla A, Dutt S, McAuley JR Dexamethasone and lenalidomide have distinct functional effects on erythropoiesis. Blood, 2011-04-28;118(8):2296-304. 2011-04-28 [PMID: 21527522] (Human)		Human
Dod HS, Bhardwaj R, Sajja V, Weidner G, Hobbs GR, Konat GW, Manivannan S, Gharib W, Warden BE, Nanda NC, Beto RJ, Ornish D, Jain AC Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis. Am. J. Cardiol., 2010-02-01;105(3):362-7. 2010-02-01 [PMID: 20102949] (Human)		Human
Ziesche E, Scheiermann P, Bachmann M, Sadik CD, Hofstetter C, Zwissler B, Pfeilschifter J, Muhl H Dexamethasone suppresses interleukin-22 associated with bacterial infection in vitro and in vivo. Clin. Exp. Immunol., 2009-09-01;157(3):370-6. 2009-09-01 [PMID: 19664145] (Human)		Human
Challier JC, Basu S, Bintein T, Minium J, Hotmire K, Catalano PM, Hauguel-De Mouzon S Obesity in pregnancy stimulates macrophage accumulation and inflammation in the placenta. Placenta, 2008-02-11;29(3):274-81. 2008-02-11 [PMID: 18262644] (Human)		Human
Hu P, Han Z, Couvillon AD, Kaufman RJ, Exton JH Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha-mediated NF-kappaB activation and down-regulation of TRAF2 expression. Mol. Cell. Biol., 2006-04-01;26(8):3071-84. 2006-04-01 [PMID: 16581782] (Human)		Human
Tsai MY, Hanson NQ, Straka RJ, Hoke TR, Ordovas JM, Peacock JM, Arends VL, Arnett DK Effect of influenza vaccine on markers of inflammation and lipid profile. J. Lab. Clin. Med., 2005-06-01;145(6):323-7. 2005-06-01 [PMID: 15976761] (Human)		Human
Matsuda M, Kawasaki F, Inoue H, Kanda Y, Yamada K, Harada Y, Saito M, Eto M, Matsuki M, Kaku K Possible contribution of adipocytokines on diabetic neuropathy. Diabetes Res. Clin. Pract., 2004-12-01;66(0):S121-3. 2004-12-01 [PMID: 15563961] (Human)		Human
Nishishita T, Ouchi K, Zhang X, Inoue M, Inazawa T, Yoshiura K, Kuwabara K, Nakaoka T, Watanabe N, Igura K, Takahashi TA, Yamashita N A potential pro-angiogenic cell therapy with human placenta-derived mesenchymal cells. Biochem. Biophys. Res. Commun., 2004-12-03;325(1):24-31. 2004-12-03 [PMID: 15522196] (Human)		Human
Birdsall HH, Porter WJ, Green DM, Rubio J, Trial J, Rossen RD Impact of fibronectin fragments on the transendothelial migration of HIV-infected leukocytes and the development of subendothelial foci of infectious leukocytes. J. Immunol., 2004-08-15;173(4):2746-54. 2004-08-15 [PMID: 15294993] (Human)		Human
Kuyvenhoven JP, Molenaar IQ, Verspaget HW, Veldman MG, Palareti G, Legnani C, Moolenburgh SE, Terpstra OT, Lamers CB, van Hoek B, Porte RJ Plasma MMP-2 and MMP-9 and their inhibitors TIMP-1 and TIMP-2 during human orthotopic liver transplantation. The effect of aprotinin and the relation to ischemia/reperfusion injury. Thromb. Haemost., 2004-03-01;91(3):506-13. 2004-03-01 [PMID: 14983226] (Human)		Human
Dahaba AA, Elawady GA, Rehak PH, List WF Procalcitonin and proinflammatory cytokine clearance during continuous venovenous haemofiltration in septic patients. Anaesth Intensive Care, 2002-06-01;30(3):269-74. 2002-06-01 [PMID: 12075632] (Human)		Human
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Human TNF-alpha QuantiGlo ELISA Kit

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