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Human Osteopontin (OPN) Quantikine ELISA Kit

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human Osteopontin (OPN) Quantikine ELISA Kit Summary

Background
The Quantikine Human Osteopontin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteopontin in cell culture supernates, plasma, urine, and human milk. It contains NS0-expressed recombinant human Osteopontin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteopontin showed linear curves that were parallel to the ...standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteopontin.
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Specificity
Natural and recombinant human Osteopontin
Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
SPP1

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DOST00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Osteopontin (OPN) Quantikine ELISA Kit

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Osteopontin/OPN (DOST00)(75)

We have publications tested in 2 confirmed species: Human, Mouse.


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Human
(75)
Mouse
(1)
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Showing Publications 1 - 10 of 75. Show All 75 Publications.
Publications using DOST00 Applications Species
Yang, J;Lewis, GP;Hsiang, CH;Menges, S;Luna, G;Cho, W;Turovets, N;Fisher, SK;Klassen, H; Amelioration of Photoreceptor Degeneration by Intravitreal Transplantation of Retinal Progenitor Cells in Rats International journal of molecular sciences 2024-07-24 [PMID: 39125629] (Human) Human
Yang, Y;Jin, X;Xie, Y;Ning, C;Ai, Y;Wei, H;Xu, X;Ge, X;Yi, T;Huang, Q;Yang, X;Jiang, T;Wang, X;Piao, Y;Jin, X; The CEBPB+ glioblastoma subcluster specifically drives the formation of M2 tumor-associated macrophages to promote malignancy growth Theranostics 2024-07-02 [PMID: 38994023] (Human) Human
Lischka, J;Schanzer, A;de Gier, C;Greber-Platzer, S;Zeyda, M; Macrophage-associated markers of metaflammation are linked to metabolic dysfunction in pediatric obesity Cytokine 2023-09-18 [PMID: 37729736] (Human) Human
Bhutada, S;Tran-Lundmark, K;Kramer, B;Conner, P;Lowry, AM;Blackstone, E;Frenckner, B;Mesas-Burgos, C;Apte, SS; Identification of protein biomarkers associated with congenital diaphragmatic hernia in human amniotic fluid Scientific reports 2023-09-19 [PMID: 37726509] (Human) Human
Zhao, M;Toma, K;Kinde, B;Li, L;Patel, AK;Wu, KY;Lum, MR;Tan, C;Hooper, JE;Kriegstein, AR;La Torre, A;Liao, YJ;Welsbie, DS;Hu, Y;Han, Y;Duan, X; Osteopontin drives retinal ganglion cell resiliency in glaucomatous optic neuropathy Cell reports 2023-08-23 [PMID: 37624696] (Human) Human
Wirestam, L;Nyberg, PB;Dzhendov, T;Gasslander, T;Sandström, P;Sjöwall, C;Björnsson, B; Plasma Osteopontin Reflects Tissue Damage in Acute Pancreatitis Biomedicines 2023-06-03 [PMID: 37371722] (Human) Human
Downes, KJ;Zuppa, AF;Sharova, A;Neely, MN; Optimizing Vancomycin Therapy in Critically Ill Children: A Population Pharmacokinetics Study to Inform Vancomycin Area under the Curve Estimation Using Novel Biomarkers Pharmaceutics 2023-04-25 [PMID: 37242578] (Human) Human
Pl�mers, R;Lindenkamp, C;Osterhage, MR;Knabbe, C;Hendig, D; Matrix Metalloproteinases Contribute to the Calcification Phenotype in Pseudoxanthoma Elasticum Biomolecules 2023-04-12 [PMID: 37189419] (Human) Human
Y Qiu, X Shen, O Ravid, D Atrakchi, D Rand, AE Wight, HJ Kim, S Liraz-Zalt, I Cooper, M Schnaider, H Cantor Definition of the contribution of an Osteopontin-producing CD11c+ microglial subset to Alzheimer's disease Proceedings of the National Academy of Sciences of the United States of America, 2023-02-02;120(6):e2218915120. 2023-02-02 [PMID: 36730200] (Human) Human
SI Karampatso, G Paltoglou, SM Genitsarid, P Kassari, E Charmandar The Effect of a Comprehensive Life-Style Intervention Program of Diet and Exercise on Four Bone-Derived Proteins, FGF-23, Osteopontin, NGAL and Sclerostin, in Overweight or Obese Children and Adolescents Nutrients, 2022-09-13;14(18):. 2022-09-13 [PMID: 36145151] (Human) Human
Show All 75 Publications.

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Bioinformatics

Gene Symbol SPP1