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HspBP1 Antibody (OTI1D5) - Azide and BSA Free

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Western Blot: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HspBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HspBP1.
Western Blot: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HspBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - Staining of paraffin-embedded Human lymphoma tissue using anti-HspBP1 mouse monoclonal antibody.
Western Blot: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - Analysis of extracts (35ug) from 9 different cell lines by using anti-HspBP1 monoclonal antibody.
Immunohistochemistry: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - Staining of paraffin-embedded Human endometrium tissue using anti-HspBP1 mouse monoclonal antibody.
Immunohistochemistry: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - Staining of paraffin-embedded Human pancreas tissue using anti-HspBP1 mouse monoclonal antibody.
CyTOF-ready: HspBP1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-71761] - HEK293T cells transfected with either HspBP1 Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI1D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71761AF350
Alexa Fluor 405
NBP2-71761AF405
Alexa Fluor 488
NBP2-71761AF488
Alexa Fluor 532
NBP2-71761AF532
Alexa Fluor 594
NBP2-71761AF594
Alexa Fluor 647
NBP2-71761AF647
Alexa Fluor 700
NBP2-71761AF700
Alexa Fluor 750
NBP2-71761AF750
Allophycocyanin
NBP2-71761APC
Allophycocyanin/Cy7
NBP2-71761APCCY7
Biotin
NBP2-71761B
CoraFluor 1
NBP2-71761CL1
DyLight 350
NBP2-71761UV
DyLight 405
NBP2-71761V
DyLight 488
NBP2-71761G
DyLight 550
NBP2-71761R
DyLight 594
NBP2-71761DL594
DyLight 650
NBP2-71761C
DyLight 680
NBP2-71761FR
DyLight 755
NBP2-71761IR
FITC
NBP2-71761F
HRP
NBP2-71761H
Janelia Fluor 525
NBP2-71761JF525
Janelia Fluor 549
NBP2-71761JF549
Janelia Fluor 646
NBP2-71761JF646
Janelia Fluor 669
NBP2-71761JF669
PE
NBP2-71761PE
PE/Atto594
NBP2-71761PEATT594
PE/Cy5.5
NBP2-71761PECY55
PE/Cy7
NBP2-71761PECY7
PerCP
NBP2-71761PCP
Unconjugated
NBP2-01168
mFluor Violet 450 SE
NBP2-71761MFV450
mFluor Violet 500 SE
NBP2-71761MFV500
mFluor Violet 610 SE
NBP2-71761MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-01168
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HspBP1 Antibody (OTI1D5) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human HSPBP1(NP_036399) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HSPBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
39.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for HspBP1 Antibody (OTI1D5) - Azide and BSA Free

  • FES1
  • Heat shock protein-binding protein 1
  • Hsp70 binding protein 1
  • hsp70 interacting protein
  • hsp70-binding protein 1
  • Hsp70-binding protein 2
  • Hsp70-interacting protein 1
  • Hsp70-interacting protein 2
  • HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
  • HSPBP
  • HspBP1
  • hspBP2

Background

Heat shock protein binding protein is an Hsp70-interacting protein that was first isolated from a human heart cDNA library using the yeast two-hybrid system. HspBP1 binds the ATPase domain of Hsp70, and inhibits Hsp70 chaperone activity. More recently HspBP1 was found to promote nucleotide dissociation from Hsc70 and is capable of altering the confirmation of the Hsp70 ATPase domain. HspBP1 is capable of regulating any Hsp70 activity that requires ATPase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HSPBP1