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Hsp47 Antibody (950811) [Unconjugated]

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HSP47 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, A549 human lung carcinoma cell line, and OVCAR-3 human ovarian carcinoma cell line. PVDF ...read more
HSP47 was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 10 µg/mL for 3 hours at room temperature. ...read more
HSP47 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HSP47 Monoclonal Antibody (Catalog # MAB91661) at 25 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
950811
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB91661-SP
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Hsp47 Antibody (950811) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human HSP47
Ala19-Asp412
Accession # P50454
Specificity
Detects human HSP47 in direct ELISAs and Western blots.  In Western blots, no cross-reactivity with mouse HSP47 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SERPINH1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB91661 in the following applications:
Publications
Read Publication using
MAB91661 in the following applications:

  • ICC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hsp47 Antibody (950811) [Unconjugated]

  • (collagen binding protein 1)
  • Arsenic-transactivated protein 3
  • AsTP3
  • BERF-1
  • CBP1
  • CBP2
  • CBP2RA-A47
  • Cell proliferation-inducing gene 14 protein
  • Collagen-binding protein
  • colligen
  • Colligin
  • colligin-1
  • colligin-2
  • gp46
  • HSP47
  • HSP47serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • member 1, (collagen binding protein 1)
  • member 2
  • member 2, (collagen-binding protein 2)
  • OI10
  • PPROM
  • proliferation-inducing gene 14
  • RA-A47
  • rheumatoid arthritis antigen A-47,47 kDa heat shock protein
  • Rheumatoid arthritis-related antigen RA-A47
  • serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47)
  • Serpin H1
  • serpin peptidase inhibitor, clade H (heat shock protein 47), member 1
  • SERPINH1
  • SERPINH2colligin

Background

Heat Shock Protein 47 (HSP47), also known as Serpin-H1/CBP1/CBP2, is localized to endoplasmic reticulum (ER), where it is a collagen-specific molecular chaperone.  In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X.  Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas.  Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.
  1. Christiansen HE, et al, (2010) Am. J. Hum. Genet. 86:3892.
  2. Tasab M, et al, (2000) EMBO J. 19:22043.
  3. Kwon YJ, et al, (2009) Oncol Res. 18:1414.
  4. Zhu J, et al, (2015) Cancer Res. 75:15805.
  5. Nese N, et al, (2010) Anal Quant Cytol Histol. 32:90

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Hsp47 Antibody
(950811) [Unconjugate
MAB91661
Hsp47 Antibody (950811) [Unconjugated]
Species: Hu
Applications: WB, IHC, ICC/IF

Publications for Hsp47 Antibody (MAB91661)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC.


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ICC
(1)
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(1)
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Review for Hsp47 Antibody (MAB91661) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB91661:
Filter by Applications
IHC-P
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Maximilian Haist
 IHC-P Human 04/03/2023
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedhuman tonsil
SpeciesHuman
Lotckfm0221121

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***

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Recent Reviews

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Immunohistochemistry-Paraffin Hsp47 MAB91661
Enlarge
 Maximilian Haist
04/03/2023
Application: IHC-P
Species: Human
read this review

Bioinformatics

Gene Symbol SERPINH1
Uniprot