HSP27 Antibody [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically up-regulated in response to cellular stress. Elevated levels of HSPs have been observed in association with ischemia/reperfusion, cancer, and chronic heart failure. HSP27, also known as HSPB1, is a member of the small heat shock protein family, which also includes HSP25 and the alpha -crystallins. HSP27 forms a large oligomer and the extent of phosphorylation plays a role in determining specific functions. HSP27 also functions as an anti-apoptotic molecule, regulating apoptosis through direct interaction with key components of the apoptotic pathway. HSP27 binds and sequesters cytochrome c released from the mitochondria in response to an apoptotic stimulus. This prevents the proper assembly of the apoptosome and subsequently, the activation of procaspase-9 and procaspase-3. Full length human HSP27 shares 83% and 81% aa identity with mouse and rat HSP27, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, Simple Western, Western Blot.

Showing Publications 1 - 6 of 6.

Publications using AF1580	Applications	Species
Li J, Jaiswal MK, Chien JF et al. Divergent single cell transcriptome and epigenome alterations in ALS and FTD patients with C9orf72 mutation Nat Commun 2023-09-15 [PMID: 37714849] (Simple Western)	Simple Western
Agnes Schröder, Kathrin Wagner, Fabian Cieplik, Gerrit Spanier, Peter Proff, Christian Kirschneck Einfluss der Phosphorylierung des Hitzeschockproteins 27 auf das Expressionsprofil von parodontalen Ligamentfibroblasten bei mechanischer Belastung Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie 2023-04-01 [PMID: 35445818]
Shahida Shafi, Rosalind Codrington, Lewis Michael Gidden, Gordon Ashley Anthony Ferns Increased expression of phosphorylated forms of heat-shock protein-27 and p38MAPK in macrophage-rich regions of fibro-fatty atherosclerotic lesions in the rabbit International Journal of Experimental Pathology 2016-02-01 [PMID: 26853073]
Banerjee S, Lin CF, Skinner KA, Schiffhauer LM, Peacock J, Hicks DG, Redmond EM, Morrow D, Huston A, Shayne M, Langstein HN, Miller-Graziano CL, Strickland J, O'Donoghue L, De AK Heat shock protein 27 differentiates tolerogenic macrophages that may support human breast cancer progression. Cancer Res., 2011-01-11;71(2):318-27. 2011-01-11 [PMID: 21224361] (Flow Cytometry, Human)	Flow Cytometry	Human
Graner MW, Cumming RI, Bigner DD The heat shock response and chaperones/heat shock proteins in brain tumors: surface expression, release, and possible immune consequences. J. Neurosci., 2007-10-17;27(42):11214-27. 2007-10-17 [PMID: 17942716] (Western Blot, Human)	Western Blot	Human
Takai S, Tokuda H, Matsushima-Nishiwaki R, Hanai Y, Kato K, Kozawa O Phosphatidylinositol 3-kinase/Akt plays a role in sphingosine 1-phosphate-stimulated HSP27 induction in osteoblasts. J. Cell. Biochem., 2006-08-01;98(5):1249-56. 2006-08-01 [PMID: 16514645] (Western Blot, Mouse)	Western Blot	Mouse

Array AF1580

• HSP27 Antibodies
• HSP27 Antibody Pair
• HSP27 ELISA Kits
• HSP27 Lysate
• HSP27 Proteins
• HSP27 Proteins and Enzyme
• HSP27 ELISAs