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Rat Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

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ELISA: Rat Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric) [NBP3-00480] - Samples were spiked with high concentrations of Rat Hormone-sensitive Lipase/HSL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric) [NBP3-00480] - Samples were spiked with high concentrations of Rat Hormone-sensitive Lipase/HSL and diluted with Reference Standard & Sample Diluent to ...read more
ELISA: Rat Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric) [NBP3-00480] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.08 - 5 ng/mL
Sensitivity
0.05 ng/mL

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Rat Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Hormone-sensitive Lipase/HSL in samples. No significant cross-reactivity or interference between Rat Hormone-sensitive Lipase/HSL and analogues was observed.
Standard Curve Range
0.08 - 5 ng/mL
Sensitivity
0.05 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.77%
Intra-Assay
4.97%
Spike Recovery
85 - 103%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
LIPE

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric)

  • hormone-sensitive lipase testicular isoform
  • Hormonesensitive Lipase
  • Hormone-sensitive Lipase
  • HSL
  • HSLEC 3.1.1.79
  • LHS
  • lipase, hormone-sensitive
  • LIPE

Background

Hormone-sensitive lipase (HSL) is an intracellular neutral lipase found in a variety of tissues, but primarily in adipose and steroidogenic tissues. It hydrolyzes triglycerides into free fatty acids and cholesteryl esters into free cholesterol for steroid hormone synthesis.

HSL is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue, and HSL antibodies are useful tools for lipid and metabolism research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

  SDS
  Rat Hormone-sensitive Lipase/HSL ELISA Kit (Colorimetric) Manual
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol LIPE