Histone H2AX Antibody

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Biological Strategies: Western Blot: Histone H2AX Antibody [NB100-638] - Downregulation of the transcription factors Slug and ZEB1 reverses the EMT programme induced by H2A.X deficiency. Immunoblot analysis of ...read more
Genetic Strategies: Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody [NB100-638] - Asynchronous wild-type (+/+) and H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at 2 ug/ml.
Genetic Strategies: Western Blot: Histone H2AX Antibody [NB100-638] - Human 293T and wild-type (+/+) or H2AX knockout (-/-) mouse embryonic fibroblasts. Antibody used at the indicated concentrations.
Western Blot: Histone H2AX Antibody [NB100-638] - Downregulation of the transcription factors Slug and ZEB1 reverses the EMT programme induced by H2A.X deficiency. Control cells (shCTRL) and cells silenced for H2A.X ...read more
Biological Strategies: Western Blot: Histone H2AX Antibody [NB100-638] - H2A.X re-expression inhibits EMT and promotes metastatic colonization in the lung. Immunoblot analysis of EMT markers in HCT116 parental ...read more
Western Blot: Histone H2AX Antibody [NB100-638] - Knockdown of CAPER induces DSB proteins ATM & H2AX & leads to apoptosis in MDA-MB-231 cells(A) H2AX phosphorylation on ser139 is significantly increased (upper right ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Histone H2AX Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
H2AX
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:400-1:800
  • Immunohistochemistry 1:400 to 1:800
  • Immunohistochemistry-Frozen 1:400 to 1:800
  • Knockout Validated
  • Western Blot 1:5000-1:25000
Application Notes
IHC-frozen sections was reported in scientific literature (PMID: 23759307). ICC/IFwas reported in scientific literature (PMID: 23759307).
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Histone H2AX Overexpression Lysate
Publications
Read Publications using
NB100-638 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H2AX Antibody

  • H2A.X
  • H2A/X
  • H2AFX
  • Histone H2AX

Background

Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family. It contains an evolutionary conserved SQ motif at the C-terminus in eukaryotes. The response of eukaryotic cells to double-strand breaks in genomic DNA includes the sequestration of many factors into nuclear foci. H2AX becomes extensively phosphorylated within 1-3 minutes of DNA damage and forms foci at break sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Histone H2AX Antibody (NB100-638)(29)

We have publications tested in 3 confirmed species: Human, Mouse, Chinese Hamster.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(4)
IF/IHC
(2)
IHC-Fr
(1)
WB
(6)
All Applications
Filter By Species
Human
(9)
Mouse
(4)
Chinese Hamster
(1)
All Species
Showing Publications 1 - 10 of 29. Show All 29 Publications.
Publications using NB100-638 Applications Species
Konopka A, Whelan DR, Jamali MS et al. Impaired NHEJ repair in amyotrophic lateral sclerosis is associated with TDP-43 mutations Molecular neurodegeneration 2020-09-09 [PMID: 32907630] (ICC/IF, Chinese Hamster, Mouse) ICC/IF Chinese Hamster, Mouse
Chilewski SD, Bhosale D, Dees S et al. Development of CAPER peptides for the treatment of triple negative breast cancer Cell Cycle 2020-01-13 [PMID: 31931653] (Human) Human
Mayra Paolillo, Raffaella Colombo, Massimo Serra, Laura Belvisi, Adele Papetti, Emilio Ciusani, Sergio Comincini, Sergio Schinelli Stem-Like Cancer Cells in a Dynamic 3D Culture System: A Model to Study Metastatic Cell Adhesion and Anti-Cancer Drugs Cells 2019-11-13 [PMID: 31766310]
Vanzo R, Bartkova J, Merchut-Maya JM et al. Autophagy role(s) in response to oncogenes and DNA replication stress Cell Death Differ. 2019-08-14 [PMID: 31409894] (WB, Human) WB Human
Campbell MC, Pontiggia L, Russell AY et al. CAPER as a therapeutic target for triple negative breast cancer Oncotarget 2018-07-13 [PMID: 30100993] (WB, Human) WB Human
Pelz L, Purfurst B, et al. The cell adhesion molecule BT-IgSF is essential for a functional blood-testis barrier and male fertility in mice. J Biol Chem 2017-12-29 [PMID: 29123028] (Mouse) Mouse
Choe KN, Nicolae CM, Constantin D et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. 2016-05-04 [PMID: 27146073] (WB, Human) WB Human
Weyemi U, Redon CE, Choudhuri R et al. The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition. Nat Commun 2016-02-15 [PMID: 26876487] (WB) WB
Sharma V, Khurana S, Kubben N et al. A BRCA1-interacting lncRNA regulates homologous recombination. EMBO Rep 2015-11-01 [PMID: 26412854] (WB, Human) WB Human
Bakhoum SF, Kabeche L, Wood MD et al. Numerical chromosomal instability mediates susceptibility to radiation treatment. Nat Commun. 2015-01-22 [PMID: 25606712] (IF/IHC, Human) IF/IHC Human
Show All 29 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol H2AX
Entrez
Uniprot