Histone H2AX [p Ser139] Antibody

Licensed to Novus Biologicals LLC under U.S. Patent Nos. 6,362,317 and 6,884,873.

Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). The H2AX variant represents between 2-25% of total H2A (1-3). Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 kDa (5). As a result of DSBs, H2AX becomes phosphorylated at serine-139 and gamma H2AX is generated as a response to genomic instability (1-4). Gamma H2AX and its phosphorylation can be detected through western blotting and immunofluorescence, serving as a biomarker for DSB frequency (1-4). Ataxia telangiectasia mutated (ATM) is considered a key protein for H2AX phosphorylation (1-4). Upon the phosphorylation and formation of gamma H2AX, many repair proteins become associated including breast cancer 1 protein (BRCA1), NBS1 (Nijmegen breakage syndrome 1)/hMRE11 (human meiotic recombination 11 protein)/ hRAD50 (N/M/R) repair complex, and 53BP1 (p53 binding protein 1) (1-4). Gamma H2AX detection assays are currently utilized for a variety of purposes, from studying DNA repair to drug development and radiation biodosimetry (4).

References

1. Palla, V. V., Karaolanis, G., Katafigiotis, I., Anastasiou, I., Patapis, P., Dimitroulis, D., & Perrea, D. (2017). gamma-H2AX: Can it be established as a classical cancer prognostic factor?. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. https://doi.org/10.1177/1010428317695931

2. Kuo, L. J., & Yang, L. X. (2008). Gamma-H2AX - a novel biomarker for DNA double-strand breaks. In vivo (Athens, Greece).

3. Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic acids research. https://doi.org/10.1093/nar/gkn550

4. Redon, C. E., Weyemi, U., Parekh, P. R., Huang, D., Burrell, A. S., & Bonner, W. M. (2012). gamma-H2AX and other histone post-translational modifications in the clinic. Biochimica et biophysica acta. https://doi.org/10.1016/j.bbagrm.2012.02.021

5. H2AX: Uniprot (P16104)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Drosophila melanogaster.

We have publications tested in 9 applications: B/N, Chemotaxis, FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 161. Show All 161 Publications. Collapse Publications.

Publications using NB100-384	Applications	Species
Audet-Delage Y, St-Louis C, Minarrieta L et al. Spatiotemporal modeling of chemoresistance evolution in breast tumors uncovers dependencies on SLC38A7 and SLC46A1 Cell reports 2023-10-31 [PMID: 37792528] (FLOW, Human)	FLOW	Human
Wu D, Huang H, Chen T et al. The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination Cell discovery 2023-10-03 [PMID: 37789001]
Nonaka K, Takubo K, Aida J et al. Accelerated telomere shortening in adrenal zona reticularis in patients with prolonged critical illness Front Endocrinol (Lausanne) 2023-09-04 [PMID: 37745694] (IHC)	IHC
McCann JL, Cristini A, Law EK et al. APOBEC3B regulates R-loops and promotes transcription-associated mutagenesis in cancer Nature genetics 2023-09-21 [PMID: 37735199] (ICC/IF, Human)	ICC/IF	Human
Ershova ES, Savinova EA, Kameneva LV et al. Satellite III (1q12) Copy Number Variation in Cultured Human Skin Fibroblasts from Schizophrenic Patients and Healthy Controls Frontiers in bioscience (Landmark edition) 2023-08-31 [PMID: 37664948] (FLOW, Human)	FLOW	Human
Ershova ES, Savinova EA, Kameneva LV et al. Antipsychotics Affect Satellite III (1q12) Copy Number Variations in the Cultured Human Skin Fibroblasts International journal of molecular sciences 2023-07-10 [PMID: 37511043] (FLOW, Human)	FLOW	Human
Sun X, Gao C, Xu X et al. FBL promotes cancer cell resistance to DNA damage and BRCA1 transcription via YBX1 EMBO reports 2023-07-25 [PMID: 37489617]
Pundlik S, Sahoo S, Barik A et al. C-terminal cysteines of HRas control Erk signaling and 15-deoxy-?12,14-prostaglandin J2(15d-PGJ2) mediated inhibition of myoblast differentiation bioRxiv 2023-05-22 (IHC, Mouse)	IHC	Mouse
Zhang Z, Shayani G, Xu Y et al. Induction of Senescence by Loss of Gata4 in Cardiac Fibroblasts Cells 2023-06-17 [PMID: 37371122] (ICC/IF, Mouse) Details: 1:200 ICC/IF dilution	ICC/IF	Mouse
He D, Tao L, Cai B et al. eIF2? incites photoreceptor cell and retina damage by all-trans-retinal The Journal of biological chemistry 2023-04-07 [PMID: 37031820]
Yoshitaka Tamaki, Jay P. Ross, Paria Alipour, Charles-Étienne Castonguay, Boting Li, Helene Catoire, Daniel Rochefort, Makoto Urushitani, Ryosuke Takahashi, Joshua A. Sonnen, Stefano Stifani, Patrick A. Dion, Guy A. Rouleau, Amanda J. Myers Spinal cord extracts of amyotrophic lateral sclerosis spread TDP-43 pathology in cerebral organoids PLOS Genetics 2023-02-06 [PMID: 36745687]
Wang B, Brandenburg S, Hernandez-Segura A et al. CDK4/6 inhibition promotes senescent states with restricted secretory phenotype and reduced toxicity bioRxiv 2020-06-06 (ICC/IF, Mouse)	ICC/IF	Mouse
Boyer MJ Extracellular Vesicles in the Vasculature: Novel Means of Communication During Vascular Insult Thesis 2020-01-01 (ICC/IF)	ICC/IF
Dombrowsky AC An innovative strategy in cancer treatment: microbeam and minibeam radiation therapy Thesis 2020-01-01 (IF/IHC)	IF/IHC
Huang Y, Hertzel AV, Fish SR et al. TP53/p53 Facilitates Stress-Induced Exosome and Protein Secretion By Adipocytes Diabetes 2023-06-22 [PMID: 37347719]
So HK, Kim H, Lee J et al. Protein Arginine Methyltransferase 1 Ablation in Motor Neurons Causes Mitochondrial Dysfunction Leading to Age-related Motor Neuron Degeneration with Muscle Loss Research (Washington, D.C.) 2023-06-19 [PMID: 37342629] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Corbett CB, St Paul A, Leigh T et al. Genetic Deletion of FXR1 Reduces Intimal Hyperplasia and Induces Senescence in Vascular Smooth Muscle Cells The American journal of pathology 2023-05-01 [PMID: 37080662] (ICC/IF, Human)	ICC/IF	Human
Pai G, Roohollahi K, Rockx D et al. Genome-wide siRNA screens identify RBBP9 function as a potential target in Fanconi anaemia-deficient head-and-neck squamous cell carcinoma Communications Biology 2023-01-13 [PMID: 36639418] (WB)	WB
Coku J Elucidation Of Cancer Therapy Resistance Mechanisms Due To Altered Endoplasmic Reticulum-Mitochondria Tethering Thesis 1905-07-13 (ICC/IF, Human)	ICC/IF	Human
Nakamichi R, Hishikawa A, Chikuma S et al. DNA-damaged podocyte-CD8 T�cell crosstalk exacerbates kidney injury by altering DNA methylation Cell reports 2023-03-28 [PMID: 36989112] (IHC-P, Mouse)	IHC-P	Mouse
Yoshimoto N, Hayashi K, Hishikawa A et al. Significance of podocyte DNA damage and glomerular DNA methylation in CKD patients with proteinuria Hypertension research : official journal of the Japanese Society of Hypertension 2023-04-01 [PMID: 36646881] (IHC-P)	IHC-P
Kostyuk SV, Ershova ES, Martynov AV et al. In Vitro Analysis of Biological Activity of Circulating Cell-Free DNA Isolated from Blood Plasma of Schizophrenic Patients and Healthy Controls-Part 2: Adaptive Response Genes 2022-12-04 [PMID: 36553550] (FLOW, Human)	FLOW	Human
Lawson CD, Peel S, Jayo A et al. Nuclear fascin regulates cancer cell survival eLife 2022-08-30 [PMID: 36039640] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Karlowitz R, Stanifer ML, Roedig J et al. USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING Cell death & disease 2022-08-06 [PMID: 35933402] (WB)	WB
Lin WL, Chen JK, Wen X et al. DDX18 prevents R-loop-induced DNA damage and genome instability via PARP-1 Cell reports 2022-07-19 [PMID: 35858569] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Angie Molina, Frédéric Bonnet, Julie Pignolet, Valerie Lobjois, Sophie Bel-Vialar, Jacques Gautrais, Fabienne Pituello, Eric Agius Single-cell imaging of the cell cycle reveals CDC25B-induced heterogeneity of G1 phase length in neural progenitor cells. Development (Cambridge, England) 2022-06-13 [PMID: 35588250]
Fielder E, Wan T, Alimohammadiha G et al. Short senolytic or senostatic interventions rescue progression of radiation-induced frailty and premature ageing in mice eLife 2022-05-04 [PMID: 35507395]
Lasagni Vitar R, Triani F, Barbariga M et al. Substance P/neurokinin-1 receptor pathway blockade ameliorates limbal stem cell deficiency by modulating mTOR pathway and preventing cell senescence Stem cell reports 2022-03-10 [PMID: 35334220] (WB, Mouse)	WB	Mouse
Xu J, Gao J, Liu J et al. ZFP541 maintains the repression of pre-pachytene transcriptional programs and promotes male meiosis progression Cell reports 2022-03-22 [PMID: 35320728] (IHC-P, Mouse)	IHC-P	Mouse
Basu DS, Bhavsar R, Gulami I et al. FMRP protects the lung from xenobiotic stress by facilitating the Integrated Stress Response Journal of cell science 2022-03-23 [PMID: 35319067]
�oku J, Booth DM, Skoda J et al. Reduced ER-mitochondria connectivity promotes neuroblastoma multidrug resistance The EMBO Journal 2022-04-19 [PMID: 35211994]
Steiner S, Seleznik GM, Reding T et al. De novo expression of gastrokines in pancreatic precursor lesions impede the development of pancreatic cancer Oncogene 2022-01-26 [PMID: 35082384] (IHC-P, Human)	IHC-P	Human
Yang Y, Lu H, Chen C et al. HIF-1 Interacts with TRIM28 and DNA-PK to release paused RNA polymerase II and activate target gene transcription in response to hypoxia Nature communications 2022-01-14 [PMID: 35031618] (Chemotaxis, WB)	Chemotaxis, WB
Samivel R, Subramanian U, Ali Khan A et al. Lipopolysaccharide Enhances Genotoxicity by Activating GADD45G and NF-kappaB in Human Corneal Epithelial Cells Oxidative medicine and cellular longevity 2022-01-04 [PMID: 35028007] (WB, Human)	WB	Human
Hui Ma, Tao Li, Xuefeng Xie, Long Jiang, Jingwei Ye, Chenjia Gong, Hanwei Jiang, Suixing Fan, Huan Zhang, Baolu Shi, Beibei Zhang, Xiaohua Jiang, Yang Li, Jianteng Zhou, Jianze Xu, Xingxia Zhang, Xiaoning Hou, Hao Yin, Yuanwei Zhang, Qinghua Shi RAD51AP2 is required for efficient meiotic recombination between X and Y chromosomes Science Advances 2022-01-01 [PMID: 35020426]
Hishikawa A, Hayashi K, Kubo A et al. DNA repair factor KAT5 prevents ischemic acute kidney injury through glomerular filtration regulation iScience 2021-12-17 [PMID: 34877495]
Su C, Liao W, Ke W Et al. The Y14-P53 Regulatory Circuit in Megakaryocyte Differentiation and Thrombocytopenia iScience 2021-10-01 [PMID: 34816104] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Ikeh K, Lamkin E, Crompton A et al. REV1 Inhibition Enhances Radioresistance and Autophagy preprints 2021-09-14 [PMID: 34771454] (WB)	WB
Suwa T, Kobayashi M, Shirai Y Et al. SPINK1 as a plasma marker for tumor hypoxia and a therapeutic target for radiosensitization JCI insight 2021-11-08 [PMID: 34747365] (ICC/IF, Human)	ICC/IF	Human
Sakama S, Kurusu K, Morita M et al. An Enriched Environment Alters DNA Repair and Inflammatory Responses After Radiation Exposure Frontiers in Immunology 2021-10-22 [PMID: 34745135]
Zuo W, Chen G, Gao Z Et al. Stage-resolved Hi-C analyses reveal meiotic chromosome organizational features influencing homolog alignment Nature communications 2021-10-08 [PMID: 34625553] (ICC/IF, Mouse)	ICC/IF	Mouse
Victor J, Deutsch J, Whitaker A et al. SARS-CoV-2 triggers DNA damage response in Vero E6 cells Biochemical and Biophysical Research Communications 2021-11-01 [PMID: 34600299] (IF/IHC, Human)	IF/IHC	Human
Kim HJ, Kim TJ, Kim YG et al. Antioxidant and Antiproliferative Activity of Finasteride against Glioblastoma Cells Pharmaceutics 2021-09-06 [PMID: 34575486] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Herok M, Wawrzynow B, Maluszek MJ et al. Chemotherapy of HER2- and MDM2-Enriched Breast Cancer Subtypes Induces Homologous Recombination DNA Repair and Chemoresistance Cancers (Basel) 2021-09-07 [PMID: 34572735]
Petreus T, Cadogan E, Hughes G et al. Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response Commun Biol 2021-08-24 [PMID: 34429505] (ICC/IF)	ICC/IF
Tomchaney M, Contoli M, Mayo J Et al. Paradoxical effects of cigarette smoke and COPD on SARS-CoV-2 infection and disease BMC pulmonary medicine 2021-08-23 [PMID: 34425811] (ICC/IF)	ICC/IF
Paramos-De-Carvalho D, Martins I, Cristovao Am Et Al. Targeting senescent cells improves functional recovery after spinal cord injury Cell reports 2021-07-06 [PMID: 34233184]
Allouche J, Rachmin I, Adhikari K Et Al. NNT mediates redox-dependent pigmentation via a UVB- and MITF-independent mechanism Cell 2021-06-29 [PMID: 34233163] (IHC-P)	IHC-P
FUtterer A, Talavera-GutiErrez A, Pons T et al. Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels Cell death & disease 2021-06-21 [PMID: 34155199] (ICC/IF, Mouse)	ICC/IF	Mouse
Polverino F, Wu TD, Rojas-Quintero J et al. Metformin: Experimental and Clinical Evidence for a Potential Role in Emphysema Treatment American journal of respiratory and critical care medicine 2021-05-25 [PMID: 34033525] (IF/IHC)	IF/IHC
Yousefzadeh MJ, Flores RR, Zhu Y et al. An aged immune system drives senescence and ageing of solid organs Nature 2021-05-12 [PMID: 33981041] (WB, Mouse) Details: Immunoblotting was performed using the gamma H2AX antibody in snap-frozen livers from mice. This allowed for the level of 8-oxo-guanine to be quantized in the spleen of young mice.	WB	Mouse
van Vliet T, Varela-Eirin M, Wang B et al. Physiological hypoxia restrains the senescence-associated secretory phenotype via AMPK-mediated mTOR suppression Molecular cell 2021-03-27 [PMID: 33823141]
Schrank TP, Lenze N, Landess LP et al. Genomic heterogeneity and copy number variant burden are associated with poor recurrence-free survival and 11q loss in human papillomavirus-positive squamous cell carcinoma of the oropharynx Cancer 2021-04-05 [PMID: 33819343] (IF/IHC, Human)	IF/IHC	Human
Du W, Huang H, Wang Z et al. AXL is a key factor for cell plasticity and promotes metastasis in pancreatic cancer Mol Cancer Res 2021-04-03 [PMID: 33811159]
Uruski P, Mikula-Pietrasik J, Naumowicz E et al. Patient-Specific Variables Determine the Extent of Cellular Senescence Biomarkers in Ovarian Tumors In Vivo Biomedicines 2021-03-24 [PMID: 33805246] (IHC-P, Human)	IHC-P	Human
Bui TM, Butin-Israeli V, Wiesolek HL et al. Neutrophils Alter DNA Repair Landscape to Impact Survival and Shape Distinct Therapeutic Phenotypes of Colorectal Cancer Gastroenterology 2021-03-19 [PMID: 33753103] (Mouse)		Mouse
Binz RL, Sadhukhan R, Miousse IR et al. Dietary Methionine Deficiency Enhances Genetic Instability in Murine Immune Cells International Journal of Molecular Sciences 2021-02-27 [PMID: 33673497] (ICC/IF)	ICC/IF
Chen C, Chen J, Wang Y et al. Ferroptosis drives photoreceptor degeneration in mice with defects in all-trans-retinal clearance The Journal of biological chemistry 2020-12-17 [PMID: 33334878] (WB, Mouse)	WB	Mouse
Hindle A, Koneru B, Makena MR et al. The O6-methyguanine-DNA methyltransferase inhibitor O6-benzylguanine enhanced activity of temozolomide + irinotecan against models of high-risk neuroblastoma Anti-Cancer Drugs 2021-03-01 [PMID: 33323683] (WB, B/N)	WB, B/N
Hwang JR, Kim WY, Cho YJ et al. Chloroquine reverses chemoresistance via upregulation of p21WAF1/CIP1 and autophagy inhibition in ovarian cancer Cell death & disease 2020-12-04 [PMID: 33277461] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Araldi RP, de Melo TC, Levy D et al. LIMD2 Regulates Key Steps of Metastasis Cascade in Papillary Thyroid Cancer Cells via MAPK Crosstalk Cells 2020-11-23 [PMID: 33238381] (FLOW, Human) Details: LIMD2-knockout human thyroid cancer lines underwent flow cytometry.	FLOW	Human
Ahmed AA, Neidle S A G-Quadruplex-Binding Small Molecule and the HDAC Inhibitor SAHA (Vorinostat) Act Synergistically in Gemcitabine-Sensitive and Resistant Pancreatic Cancer Cells Sci Adv 2020-11-01 [PMID: 33227941]
Pozzi E, Giorgio E, Mancini C et al In vitro dexamethasone treatment does not induce alternative ATM transcripts in cells from Ataxia-Telangiectasia patients Sci Rep 2020-11-20 [PMID: 33214630] (WB, Mouse) Details: Citation using the HRP version of this antibody.	WB	Mouse
Balukoff NC, Ho JJD, Theodoridis PR et al. A translational program that suppresses metabolism to shield the genome Autophagy 2020-11-08 [PMID: 33188200] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Pan F, Xu X, Zhang LL et al. Dietary riboflavin deficiency induces genomic instability of esophageal squamous cells that is associated with gut microbiota dysbiosis in rats Food Funct 2020-11-02 [PMID: 33135706]
Shimura T, Nakashiro C, Narao M, Ushiyama A Induction of oxidative stress biomarkers following whole-body irradiation in mice PLoS One 2020-10-01 [PMID: 33002096] (ICC/IF, Mouse)	ICC/IF	Mouse
Sadhukhan R, Leung J. W. C, et al. Fractionated radiation suppresses Kruppel-like factor 2 pathway to a greater extent than by single exposure to the same total dose. Sci Rep 2020-05-07 [PMID: 32382091] (B/N)	B/N
Zhao J, Zhang L et al. ATM is a key driver of NF-kappa B-dependent DNA-damage-induced senescence, stem cell dysfunction and aging. Aging (Albany NY) 2020-03-22 [PMID: 32201398] (WB, Mouse)	WB	Mouse
Boye P, Floch F, Serres F et al. Randomized, double-blind trial of F14512, a polyamine-vectorized anticancer drug, compared with etoposide phosphate, in dogs with naturally occurring lymphoma Oncotarget 2020-02-18 [PMID: 32133044] (FLOW, Canine)	FLOW	Canine
Hayashi K, Hishikawa A, Hashiguchi A et al. Association of glomerular DNA damage and DNA methylation with one-year eGFR decline in IgA nephropathy Sci Rep 2020-01-14 [PMID: 31937846] (IHC-P, Human)	IHC-P	Human
Quach C, Song Y, Guo H, et al. A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice Nat Commun 2019-12-12 [PMID: 31831743] (WB, Mouse)	WB	Mouse
Pan F, Chen Y, He JZ, et al. Dietary riboflavin deficiency promotes N-nitrosomethylbenzylamine-induced esophageal tumorigenesis in rats by inducing chronic inflammation Am J Cancer Res 2019-11-01 [PMID: 31815047] (IF/IHC, Rat)	IF/IHC	Rat
Krieger KL, Hu WF, Ripperger T, Woods NT Functional Impacts of the BRCA1-mTORC2 Interaction in Breast Cancer Int J Mol Sci 2019-11-23 [PMID: 31771139] (WB, Human)	WB	Human
Kim JJ, Lee SY, Gong F et al. Systematic bromodomain protein screens identify homologous recombination and R-loop suppression pathways involved in genome integrity Genes Dev. 2019-12-01 [PMID: 31753913] (Human)		Human
Lee YY, Cho YJ, Shin SW et al. Anti-Tumor Effects of Wee1 Kinase Inhibitor with Radiotherapy in Human Cervical Cancer Sci Rep 2019-10-28 [PMID: 31659268] (IHC-P, Human)	IHC-P	Human
Haeger A, Alexander S, Vullings M et al. Collective cancer invasion forms an integrin-dependent radioresistant niche J. Exp. Med. 2020-01-06 [PMID: 31658985] (WB)	WB
Dombrowsky, A C, Burger, K Et al. A proof of principle experiment for microbeam radiation therapy at the Munich compact light source. Radiat Environ Biophys 2020-03-01 [PMID: 31655869] (ICC/IF, Drosophila melanogaster)	ICC/IF	Drosophila melanogaster
Ye S, Ding YF, Jia WH et al. SET domain-containing protein 4 epigenetically controls breast cancer stem cell quiescence Cancer Res. 2019-07-15 [PMID: 31308046] (ICC/IF, Human)	ICC/IF	Human
Hu WF, Krieger KL, Lagundzin D et al. CTDP1 regulates breast cancer survival and DNA repair through BRCT-specific interactions with FANCI Cell Death Discov 2019-06-19 [PMID: 31240132] (WB, Human)	WB	Human
Li, X;Wu, L;Zopp, M;Kopelov, S;Du, W; p53-TIGAR axis-mediated glycolytic suppression attenuates DNA damage and genomic instability in Fanconi anemia hematopoietic stem cells Stem Cells 2019-04-12 [PMID: 30977208] (WB, Mouse)	WB	Mouse
Pillay N, Tighe A, Nelson L et al. DNA Replication Vulnerabilities Render Ovarian Cancer Cells Sensitive to Poly(ADP-Ribose) Glycohydrolase Inhibitors Cancer Cell 2019-03-18 [PMID: 30889383] (ICC/IF, Human)	ICC/IF	Human
Wang Y, Wang H, Zhang Y et al. Reprogramming of Meiotic Chromatin Architecture during Spermatogenesis Mol. Cell 2019-02-07 [PMID: 30735655] (ICC/IF, Mouse)	ICC/IF	Mouse
Zong D, Adam S, Wang Y et al. BRCA1 Haploinsufficiency Is Masked by RNF168-Mediated Chromatin Ubiquitylation. Mol. Cell 2019-01-10 [PMID: 30704900] (ICC/IF, Human)	ICC/IF	Human
Zhang L, Zhao J, Gurkar A et al. Methods to Quantify the NF-kb Pathway During Senescence. Methods in Molecular Biology. 2018-11-25 [PMID: 30474851] (IF/IHC)	IF/IHC
Yousefzadeh MJ, Melos KI, Angelini L et al. Mouse Models of Accelerated Cellular Senescence. Methods Mol. Biol. 1905-07-11 [PMID: 30474850] (Mouse)		Mouse
Wu J, Xiao S, Yuan M et al. PARP inhibitor re-sensitizes Adriamycin resistant leukemia cells through DNA damage and apoptosis. Mol. Carcinog. 2018-11-20 [PMID: 30431088] (WB, Human)	WB	Human
Simoes-Sousa S, Littler S, Thompson SL et al. The p38a Stress kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1a. Cell Rep. 2018-10-16 [PMID: 30332653] (WB, Human)	WB	Human
Mata-Garrido J, Tapia O, Casafont I et al. Persistent accumulation of unrepaired DNA damage in rat cortical neurons: nuclear organization and ChIP-seq analysis of damaged DNA. Acta Neuropathol Commun 2018-07-26 [PMID: 30049290] (Chemotaxis, Rat)	Chemotaxis	Rat
Philion C, Ma D, Ruvinov I et al. Cymbopogon citratus and Camellia sinensis extracts selectively induce apoptosis in cancer cells and reduce growth of lymphoma xenografts in vivo. Oncotarget 2017-11-18 [PMID: 29340014] (IHC-Fr, Human)	IHC-Fr	Human
Affronti HC, Long MD, Rosario SR et al. Dietary folate levels alter the kinetics and molecular mechanism of prostate cancer recurrence in the CWR22 model. Oncotarget 2017-11-28 [PMID: 29262598] (WB, Mouse)	WB	Mouse
Long J, Huang C, Chen Y et al. Telomeric TERB1-TRF1 interaction is crucial for male meiosis Nat. Struct. Mol. Biol. 2017-10-30 [PMID: 29083416] (Mouse)		Mouse
Mateos-Gomez PA, kent T, Deng Sk et al. The helicase domain of Poltheta counteracts RPA to promote alt-NHEJ. Nat. Struct. Mol. Biol. 2017-12-01 [PMID: 29058711] (ICC/IF, Mouse)	ICC/IF	Mouse
Boege Yannick, Malehmir Mohsen, Healy Marc E et al. A Dual Role of Caspase-8 in Triggering and Sensing ProlifeRation-Associated DNA Damage, a Key Determinant of Liver Cancer Development. Cancer Cell 2017-01-01 [PMID: 28898696] (ICC/IF, Mouse)	ICC/IF	Mouse
Aguilera KY, Huang H, Du W et al. Inhibition of discoidin domain receptor 1 reduces collagen-mediated tumorigenicity in pancreatic ductal adenocarcinoma Mol. Cancer Ther. 2017-09-01 [PMID: 28864681] (Human)		Human
Yao C, Carraro G, konda B et al. Sin3a regulates epithelial progenitor cell fate during lung development. Development. 2017-07-15 [PMID: 28619823] (ICC/IF, Mouse)	ICC/IF	Mouse
Jiang L, Li T, Zhang X et al. RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. Curr. Biol. 2017-05-22 [PMID: 28502657]
Valtcheva N, Lang FM, Noske A et al. Tracking the origin of simultaneous endometrial and ovarian cancer by next-generation sequencing - a case report. BMC Cancer 2017-01-19 [PMID: 28103826] (IF/IHC, Human)	IF/IHC	Human
Piwko W, Mlejnkova LJ, Mutreja K et al. The MMS22L-TONSL heterodimer directly promotes RAD51-dependent recombination upon replication stress. EMBO J. 2016-10-26 [PMID: 27797818]
Oe S, Miyagawa K, Honma Y, Harada M. Copper induces hepatocyte injury due to the endoplasmic reticulum stress in cultured cells and patients with Wilson disease. Exp. Cell Res. 2016-08-05 [PMID: 27502587]
Mata-Garrido J, Casafont I, Tapia O et al. Neuronal accumulation of unrepaired DNA in a novel specific chromatin domain: structural, molecular and transcriptional characterization. Acta Neuropathol Commun 2016-04-22 [PMID: 27102221] (ICC/IF, Rat)	ICC/IF	Rat
Dahlhoff M, Camera E, Schafer M et al. Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis, and ocular integrity in mice. Development. 2016-03-17 [PMID: 26989175] (IHC-P, Mouse)	IHC-P	Mouse
Chen L, Peng Z, Meng Q et al. Loss of IkB kinase beta promotes myofibroblast transformation and senescence through activation of the ROS-TGFbeta autocrine loop Protein Cell 2016-03-05 [PMID: 26946493] (WB, Mouse)	WB	Mouse
Francia S, Cabrini M, Matti V et al. DICER, DROSHA and DNA damage-response RNAs are necessary for the secondary recruitment of DNA damage response factors J. Cell. Sci. 2016-02-16 [PMID: 26906421] (ICC/IF, Human)	ICC/IF	Human
Kim Jeongkyu, Sturgill David, Tran Andy D et al. Controlled DNA double-strand break induction in mice reveals post-damage transcriptome stability. Nucleic Acids Res 2016-01-01 [PMID: 26687720] (FLOW, Mouse)	FLOW	Mouse
Phaeton R, Jiang Z, Revskaya E et al. Beta emitters rhenium-188 and lutetium-177 are equally effective in radioimmunotherapy of HPV-positive experimental cervical cancer. Cancer Med. 2015-12-02 [PMID: 26625938] (IF/IHC, Mouse)	IF/IHC	Mouse
Cristini A, Park JH, Capranico G et al. DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions. Nucleic Acids Res. 2015-11-17 [PMID: 26578593] (ICC/IF, WB, Human)	ICC/IF, WB	Human
Markova E, Somsedikova A, Vasilyev S et al. DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy. Int. J. Radiat. Biol. 2015-11-04 [PMID: 26537244] (ICC/IF, Human)	ICC/IF	Human
Campbell A, Krupp B, Bushman J et al. A novel mouse model for ataxia-telangiectasia with a N-terminal mutation displays a behavioral defect and a low incidence of lymphoma but no increased oxidative burden. Hum. Mol. Genet. 2015-08-26 [PMID: 26310626] (ICC/IF, Mouse)	ICC/IF	Mouse
Durdik M, Kosik P, Gursky J et al. Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and gammaH2AX foci in human lymphocytes. Cytometry A. 2015-08-04 [PMID: 26243567] (ICC/IF, FLOW, Human)	ICC/IF, FLOW	Human
Tierny D, Serres F, Segaoula Z et al. Phase 1 clinical pharmacology study of F14512, a new polyamine-vectorized anti-cancer drug, in naturally occurring canine lymphoma Clin. Cancer Res. 2015-07-13 [PMID: 26169968] (FLOW, Human)	FLOW	Human
Chen WT, Ebelt ND, Stracker TH et al. ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion. Elife 2015-01-01 [PMID: 26030852] (WB)	WB
Reid DA, Keegan S, Leo-Macias A et al. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proc Natl Acad Sci U S A 2015-05-19 [PMID: 25941401] (ICC/IF, Human)	ICC/IF	Human
Mateos-Gomez PA, Gong F, Nair N et al. Mammalian polymerase Theta promotes alternative NHEJ and suppresses recombination Nature. 2015-02-12 [PMID: 25642960] (ICC/IF, Human, Mouse)	ICC/IF	Human, Mouse
So EY, Ouchi T. Translational initiation regulated by ATM in dendritic cells development. Cell Death Dis. 2014-09-12 [PMID: 25210801]
Bakhoum SF, Kabeche L, Murnane JP et al. DNA damage response during mitosis induces whole chromosome mis-segregation. Cancer Discov. 2014-08-08 [PMID: 25107667]
Wang X, Liu S, Zhao Q et al. Three-dimensional hydrogel scaffolds facilitate in-vitro self-renewal of human skin-derived precursors. Acta Biomater 2014-03-27 [PMID: 24681373] (ICC/IF, Human)	ICC/IF	Human
Errington TM, Macara IG. Depletion of the Adaptor Protein NCK Increases UV-Induced p53 Phosphorylation and Promotes Apoptosis. PLoS One. 2013-09-23 [PMID: 24086708] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Kabeche L, Compton DA. Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation. Nature. 2013-10-03 [PMID: 24013174] (ICC/IF, Human)	ICC/IF	Human
Gascoigne KE, Cheeseman IM. Induced dicentric chromosome formation promotes genomic rearrangements and tumorigenesis. Chromosome Res 2013-07-01 [PMID: 23793898] (ICC/IF, Mouse)	ICC/IF	Mouse
Sorokina S, Markova E, Gursky J et al. Relative biological efficiency of protons at low and therapeutic doses in induction of 53BP1/gammaH2AX foci in lymphocytes from umbilical cord blood. Int J Radiat Biol 2013-05-22 [PMID: 23607485] (ICC/IF, Human)	ICC/IF	Human
Luo Y, Deng X, Cheng F, Li Y, Qiu J. SMC1-mediated intra-S phase arrest facilitates Bocavirus DNA replication. J Virol 2013-01-30 [PMID: 23365434] (FLOW, Canine)	FLOW	Canine
Fung C, Pupo GM, Scolyer RA et al. p16(INK) (4a) deficiency promotes DNA hyper-replication and genetic instability in melanocytes. Pigment Cell Melanoma Res. 2013-03-01 [PMID: 23279822] (ICC/IF, Human)	ICC/IF	Human
Vasilyev SA, Kubes M, Markova E, Belyaev I. DNA damage response in CD133 + stem/progenitor cells from umbilical cord blood: Low level of endogenous foci and high recruitment of 53BP1 Int J Radiat Biol 2013-01-08 [PMID: 23206244] (ICC/IF, Human)	ICC/IF	Human
Bandyopadhyay K, Li P, Gjerset RA. CK2-Mediated Hyperphosphorylation of Topoisomerase I Targets Serine 506, Enhances Topoisomerase I-DNA Binding, and Increases Cellular Camptothecin Sensitivity PLoS One 2012-01-01 [PMID: 23185622] (WB, Human)	WB	Human
Douarre C, Sourbier C, Dalla Rosa I et al. Mitochondrial Topoisomerase I is Critical for Mitochondrial Integrity and Cellular Energy Metabolism plos ONE 2012-01-01 [PMID: 22911747] (IF/IHC, WB, ICC/IF, Mouse)	IF/IHC, WB, ICC/IF	Mouse
Mu X, Wen J, Guo M et al. Retinoic acid derived from the fetal ovary initiates meiosis in mouse germ cells J Cell Physiol 2013-03-01 [PMID: 22886539] (IF/IHC, Mouse)	IF/IHC	Mouse
Mok MT, Henderson BR. Three-dimensional imaging reveals the spatial separation of ?H2AX-MDC1-53BP1 and RNF8-RNF168-BRCA1-A complexes at ionizing radiation-induced foci. Radiother Oncol. 2012-05-23 [PMID: 22633816] (ICC/IF, Human)	ICC/IF	Human
Kelsall IR, Langenick J, MacKay C et al. The Fanconi anaemia components UBE2T and FANCM are functionally linked to nucleotide excision repair. PLoS One 2012-01-01 [PMID: 22615860]
Jiang G, Zou Y, Wu X et al. Replication-mediated disassociation of replication protein A-XPA complex upon DNA damage: implications for RPA handing off. Cell Biol Int 2012-08-01 [PMID: 22578086]
Westendorp B, Mokry M, Groot Koerkamp MJA et al. E2F7 represses a network of oscillating cell cycle genes to control S-phase progression. Nucleic Acids Research. 2011-12-17 [PMID: 22180533]
Wei PC, Lo WT, Su MI et al. Non-targeting siRNA induces NPGPx expression to cooperate with exoribonuclease XRN2 for releasing the stress. Nucleic Acids Res 2012-01-01 [PMID: 21908404]
Mohapatra S, Kawahara M, Khan IS et al. Restoration of G1 chemo/radioresistance and double-strand-break repair proficiency by wild-type but not endonuclease-deficient Artemis Nucleic Acids Res 2011-08-01 [PMID: 21531702]
Redon CE, Nakamura AJ, Sordet O et al. ?-H2AX Detection in Peripheral Blood Lymphocytes, Splenocytes, Bone Marrow, Xenografts, and Skin. Methods Mol Biol;682:249-70. 2011-01-01 [PMID: 21057933]
Suzuki K, Yamauchi M, Oka Y et al. A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. Nucleic Acids Res 2010-07-01 [PMID: 20385602]
Thompson SL, Compton DA. Proliferation of aneuploid human cells is limited by a p53-dependent mechanism. J Cell Biol;188(3):369-81. 2010-02-08 [PMID: 20123995] (ICC/IF, Human)	ICC/IF	Human
Buonomo et al. J.Cell Biol. 187 (3):385-398. 2009-01-01 [PMID: 19948482]
Brouwer Anneke K, Schimmel Joost, Wiegant Joop C A G et al. Telomeric DNA mediates de novo PML body formation. Mol Biol Cell. 2009-11-01 [PMID: 19793919]
Mok MT, Henderson BR. A comparison of BRCA1 nuclear localization with 14 DNA damage response proteins and domains: identification of specific differences between BRCA1 and 53BP1 at DNA damage-induced foci. Cell Signal 2010-01-01 [PMID: 19766185] (Human)		Human
Ordinario et al. J.Immunol. 183 (7):4545-4553. 2009-01-01 [PMID: 19748985]
De Nicolo A, Parisini E, Zhong Q et al. Multimodal assessment of protein functional deficiency supports pathogenicity of BRCA1 p.V1688del. Cancer Res 2009-09-01 [PMID: 19706752]
Sankar et al. J.Biol.Chem 284 (22):15193-15205. 2009-01-01 [PMID: 19332536]
Nichols GJ, Schaack J, Ornelles DA. Widespread phosphorylation of histone H2AX by species C adenovirus infection requires viral DNA replication. J Virol 2009-06-01 [PMID: 19321613]
Rai et al. PNAS 106 (1):169-174. 2009-01-01 [PMID: 19118192]
Rieske P, Augelli BJ, Stawski R et al. A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells. Exp Cell Res 2009-02-01 [PMID: 19061885] (Human)		Human
Wang X, Kam Z, Carlton PM et al. Rapid telomere motions in live human cells analyzed by highly time-resolved microscopy. Epigenetics Chromatin 2008-01-01 [PMID: 19014413] (Human)		Human
Eliezer et al. J.Biol.Chem 284 (1):426-435. 2009-01-01 [PMID: 18986980]
Ewald et al. Cancer Res 68 (19):7947-7955. 2008-01-01 [PMID: 18829552]
Rainey et al. Cancer Res 68 (18):7466-7474. 2008-01-01 [PMID: 18794134]
Greubel C, Hable V, Drexler GA et al. Quantitative analysis of DNA-damage response factors after sequential ion microirradiation Radiat Environ Biophys. 2008-11-01 [PMID: 18648840]
Greubel C, Hable V, Drexler GA et al. Competition effect in DNA damage response. Radiat Environ Biophys 2008-11-01 [PMID: 18648839] (ICC/IF, Human)	ICC/IF	Human
Chan et al. J.Biol.Chem 283 (23):15716-15723. 2008-01-01 [PMID: 18400748]
Matsuoka S, Ballif BA, Smogorzewska A et al. ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 2007-05-25 [PMID: 17525332]
Geng, L, Cuneo, K C, Fu, A, Tu, T, Atadja, P W, Hallahan, D E. Histone Deacetylase (HDAC) Inhibitor LBH589 Increases Duration of {gamma}-H2AX Foci Confines HDAC4 to the Cytoplasm in Irradiated Non-Small Cell Lung Cancer, . 66, 11298-11304. 2006-01-01 [PMID: 17145876]
Liu Y, Rusinol A, Sinensky M et al. DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A. J Cell Sci 2006-11-01 [PMID: 17062639]
Bentle MS, Reinicke KE, Bey EA et al. Calcium-dependent modulation of poly(ADP-ribose) polymerase-1 alters cellular metabolism and DNA repair. J Biol Chem 2006-11-01 [PMID: 16920718]
Hau PM, Siu WY, Wong N et al. Polyploidization increases the sensitivity to DNA-damaging agents in mammalian cells. FEBS Lett 2006-08-01 [PMID: 16887121]
Kozlov SV, Graham ME, Peng C et al. Involvement of novel autophosphorylation sites in ATM activation. EMBO J 2006-08-09 [PMID: 16858402] (WB, Human)	WB	Human
Marti, T M et al. H2AX phosphorylation within the G1 phase after UV irradiation depends on nucleotide excision repair not DNA double-str breaks. PNAS 103: 9891-9896. 2006-01-01 [PMID: 16788066]
Ho CC, Siu WY, Lau A et al. Stalled replication induces p53 accumulation through distinct mechanisms from DNA damage checkpoint pathways. Cancer Res 2006-02-01 [PMID: 16489026]
Hovest, MG et al. Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase persistent DNA damage foci at telomerase. Mol Biol Cell 17: 1758-1767. 2006-01-01 [PMID: 16436511]
Igoucheva O, Alexeev V, Yoon K et al. Differential cellular responses to exogenous DNA in mammalian cells and its effect on oligonucleotide-directed gene modification. Gene Ther 2006-02-01 [PMID: 16177821]
Show All 161 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Sergio Barrios	IHC	Mouse	10/05/2023	View Summary Application Immunohistochemistry Sample Tested Cancer Cells Species Mouse Lot A10
	5 reviewed by: Verified Customer	IF	Human	10/04/2013	View Summary Application Immunofluorescence Species Human

Array NB100-384

• Histone H2AX Antibodies
• Histone H2AX Lysate
• Histone H2AX Proteins
• Histone H2AX ELISA