We ordered and received the HIF-1 alpha antibody NB100-449 and on the packing slip it says that is prepared in TBS+0.1% BSA. I will be using it for western blots. DO you think my choice of blocking buffer (milk or Blotto) could interfere with the activity of this antibody or should it be necessarily BSA based?

Choice of blocking buffer is entirely within your discretion; it will not affect the antibody binding activity. Please note, some blocking buffers may work better than others and sometimes optimization is needed.When working with hypoxia there are other important factors to consider, as HIF-alpha is very easily degraded. The lysates should be freshly prepared. Also lysate preparation should be as quick as possible to avoid any exposure to oxygen - we recommend lysing cells directly into the SDS sample loading buffer (Laemmli buffer). We also highly recommend using positive control (you can prepare them yourself or choose some of those we have for sale).I have attached some additional information that you may find quite useful. Also here are some hypoxia related FAQ addressing common concerns.

Our lab recently ordered NB100-449, HIF-1 alpha antibody. Unfortunately an inexperienced technician stored it at -20C rather than 4C for approximately 2 days. Have you done any tests to determine antibody functionality if frozen?

The recommended storage condition of HIF-1 alpha antibody NB100-449 is 4C and we highly recommended not storing the product lower than the freezing point, as it may potentially disrupt the protein folding and destroy the antigen binding site of the antibody. Since we likely have not tested a storage condition of -20C for this antibody, we cannot really say if this antibody has been impaired by the storage condition. Our recommendation would be to test the antibody in a small portion of your treated cell line and see if the antibody is still reactive to the HIF-1 alpha protein.

I detect the 60 kDa band for HIF-1 alpha antibody (NB100-449), but no band at the right molecular weight. Do you know what this non-specific band is?

Most likely the 60kDa band is a degradation product of HIF1A in your samples. Since HIF-1 alpha degrades so rapidly in the presence of oxygen, our lab often sees bands at lower molecular weights. We have also experienced some non-specific banding around that molecular weight too in some tests.

Could you tell us if these antibodies need to use cell lysate treated with special extraction for WB? Do you have the recommended preparation method of cell lysate?

Usually HIF1 alpha and HIF2 alpha are hard to detect endogenously and require some inducement. The most common is hypoxic treatment of the cells. This can be also be chemically induced by CoCl2 treatment as well. Our recommended protocols and lysate preparations can be found in our white paper here

Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?

HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.

Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.

All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).

I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg?

We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.

What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?

The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.

We got the Hif1a (NB100-105) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.

HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative.Also, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)

I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?

A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.

(1) HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2) The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3) Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4) For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs (5) Last but not the least, Novus technical support team may be contacted via email

I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!

You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.

I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?

CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?

CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.

Is cross-reactivity with HIF-2 alpha tested/predicted?

Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.

Could you clarify if Yeast is a reactive species for HIF1a product #NB100-105?

This HIF-1 alpha antibody has not been tested in yeast. The homology is not significantly homologous so we do not believe there will be cross reactivity to the yeast protein.

Can HIF-1 alpha Antibody (Dylight 488), product NB100-479G, react with goat species? Does this product have preservatives in it?

NB100-479G has not been tested in goat species. Only the listed species on the product page and datasheet will be guaranteed. Reactivity: Hu, Mu, Rt, Ca, Fi, Ha, Pm, RbThe immunogen for this antibody corresponds to amino acids 530-825 of mouse Hifa. Running a sequence alignment of this sequence with the goat sequence found on UniProt yields around 81% homology.Mouse Hif1a: https://www.uniprot.org/uniprot/Q61221#sequencesGoat Hif1a: https://www.uniprot.org/uniprot/A0A023R978#sequencesLastly, there is 0.05% Sodium Azide present in the formulation of the product. This is also listed on the product page and datasheet. Buffer: 50mM Sodium BoratePreservative: 0.05% Sodium Azide

What's the difference between NBP2-75977 vs NBP2-75978?

While the same immunogen was used to make both HIF-1 alpha antibodies, they are different clones, meaning they recognize a different epitope on the immunogen .

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HIF-1 alpha Antibody

Name: HIF-1 alpha Antibody
Brand: Novus Biologicals
SKU: NB100-449
Rating: 4.3 (29 reviews)

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Knockdown Validated: HIF-1 alpha Antibody [NB100-449] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or shHIF-2alpha. The clones were cultured under normoxic (N) or hypoxic (H, 1% O2) conditions, or treated with DMOG for 6 h. 30 ug protein of cellular protein were loaded for the detection of HIF-1alpha, and nuclear extracts (20 ug protein) were used for the detection of HIF-2alpha. Image collected and cropped by CiteAb from the following publication (//biosignaling.biomedcentral.com/articles/10.1186/1478-811X-11-80) licensed under a CC-BY license.

Genetic Strategies: Knockdown Validated: HIF-1 alpha Antibody [NB100-449] - Morphological alterations induced by DMOG are HIF-1alpha dependent. glEND.2 cells were stably transfected with shGFP, shHIF-1alpha or ...read more

Biological Strategies: Western Blot: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha induction on Caki-1 cell lysate using CoCl2. Image from verified customer review.

Simple Western: HIF-1 alpha Antibody [NB100-449] - Simple Western lane view shows a specific band for HIF-1 alpha in 0.5 mg/ml of hypoxic HeLa lysate. This experiment was performed under reducing conditions using the ...read more

Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Mouse Brain, Neurons 40X

Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Detection of human HIF1-alpha by immunohistochemistry. Sample: FFPE section of renal cell carcinoma. Antibody: Affinity purified rabbit anti-HIF1-alpha antibody ...read more

Biological Strategies: Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of Human HIF-1 alpha by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Murine primary bone marrow derived macrophages stained with HIF1-alpha antibody (red). Nuclei were counterstained with DAPI (blue). Image from ...read more

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449] - Analysis of microvascular density and HIF-1alpha activity. Low, homogenous expression of HIF-1alpha was detected in the tumors of NNK treated mice (40X, ...read more

Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - HeLa cells were treated for 15 hrs with 200 uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125 ug anti-HIF-1 alpha and ...read more

Biological Strategies: Western Blot: HIF-1 alpha Antibody [NB100-449] - Analysis of HIF-1 alpha in human myeloma cell lysate using anti-HIF-1 alpha. Cells were untreated or treated with IGF-1, IL-6 or CoCl2. ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of HIF-1 alpha in a hypoxic sample. Lane 1: CoCl2 treated Cos-7 nuclear extract (hypoxic). Lane 2: Untreated Cos-7 nuclear extract (normoxic).

Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of mouse HIF-1 alpha on hypoxia treated MEFs

Biological Strategies: Western Blot: HIF-1 alpha Antibody [NB100-449] - Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells that were treated with cobalt chloride (+; 200 uM) or mock treated (-). ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - BMDM were seeded at 0.5x10^6 overnight. Cells were treated with 10 ng/ml LPS for 24 hrs. Image from verified customer review.

Western Blot: HIF-1 alpha Antibody [NB100-449] - Analysis of total protein using anti-HIF-1 alpha, -CAIX, -ISCU antibodies and VDACs antibody (PMID: 29596470).

Western Blot: HIF-1 alpha Antibody [NB100-449] - Analysis of total protein using anti-HIF-1 alpha, -ISCU, -FXN antibodies. CoCl2 was used to treat or not HeLa cells for 2 days (PMID: 29596470).

Western Blot: HIF-1 alpha Antibody [NB100-449] - Detection of human HIF1 alpha by western blot. Samples: Whole cell lysate (15 and 50 ug) from HEK293T cells that were either treated with 200 uM cobalt chloride (+) or ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - 3T3-L1 mouse embryonic fibroblast adipose-like cell line. Antibody at 1:2000. WB image submitted by a verified customer review.

Biological Strategies: Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Formaldehyde-fixed asynchronous HeLa cells.

Immunoprecipitation: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha analysis in HEK293 cells. Image from verified customer review.

Immunoprecipitation: HIF-1 alpha Antibody [NB100-449] - Detection of human HIF1 alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells that were ...read more

beta -Glucan size affects the requirement for reactive oxygen species (ROS) in IL-1 beta induction.(B) Human mDCs from healthy donors or chronic granulomatous disease (CGD) patients were stimulated with curdlan or ...read more

Western Blot: Rabbit Polyclonal HIF-1 alpha Antibody - Analysis of HIF-1 alpha antibody on mouse liver nuclear extracts. Image from a verified customer review.

HIF-1 alpha /2 alpha expression in myeloid-specific KO mice targeting the HIF pathway. (A) Images of the colon of wild-type (WT), myeloid-specific Hif-1a KO (hMRP8 Hif-1a KO) or von Hippel Lindau (Vhl) KO (hMRP8 Vhl ...read more

Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Analysis of microvascular density & HIF-1 alpha activity. Microvascular density of pulmonary tumors was significantly higher in hyperplastic (B) & tumoral ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Chronic ethanol feeding enhances tumorigenesis markers during AOM/DSS-induced tumorigenesis. AOM/DSS-induced colonic tumorigenesis was induced ...read more

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449] - Treatment with HBOT or PFD decreased HIF-1 alpha expression in the AD lesions.IHC for HIF-1 alpha (A). Scale bar is 100 µm. HIF-1 alpha + area was ...read more

Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [NB100-449] - Elevated PIK3/AKT/HIF-1 alpha pathways in KL SqCC tumours.(a,b) Analysis of TCGA gene expression (normalized TPM) & PIK3CA (a) & PTEN (b) genomic copy ...read more

Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - Immunolocalization of HIF1A in limbs.Immunoreactivity was detected in control limbs in the apical ectodermal ridge & interdigital regions (I.R.). The mesenchymal ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Effects of cell stress on VEGF-A & HIF1 alpha expression in Müller cells & Y79 photoreceptors. (A) VEGF-A was measured by ELISA using conditioned media collected from ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Phenylhydrazine administration results in hypoxia in retinal vessels & in HIF-1 alpha & HIF-2 alpha stabilization in peripheral blood leukocytes.(A) 3-dimensional ...read more

Immunohistochemistry-Frozen: HIF-1 alpha Antibody [NB100-449] - Ethanol elevates tumorigenic markers & nuclear localization of HIF1 alpha in colonic crypts during AOM/DSS-induced tumorigenesis: AOM/DSS-induced colonic ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Dectin-1-induced NADPH oxidase-derived ROS is required for augmented IL-1 beta during TSLPR inhibition. (A) Human mDC derived from healthy donors (HD) or CGD patients ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Reactive oxygen species production & expression of stabilized HIF1 alpha & AMPK subunit alpha 2 & in H1299 & H1299r cells(A) H1299, H1299r, P31 & P31r cell were seeded ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Pyruvate kinase isoform M2 (PKM2) & hypoxia-inducible factor 1 alpha (Hif-1 alpha ) bind directly to the promoter region of PDL1 in primary murine BMDM cells. BMDM cells ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Changes in Hepatic Oxygen Saturation & Hypoxia-related Protein Expression. (A) In the representative case, the hepatic oxygen saturation in the experimental group was ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - BCAT1 expression & inhibition in human macrophages.(a) RNA-sequencing in human monocyte-derived macrophages (hMDMs) shows mRNA copies of BCAT enzymes in normalized ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - HIF-1 alpha /2 alpha expression in myeloid-specific KO mice targeting the HIF pathway. (A) Images of the colon of wild-type (WT), ...read more

Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody [NB100-449] - Increased infiltration of myeloid cells expressing HIF-1 alpha in DSS-induced colitis. (A) Western blot analysis of MRP8, F4/80 or actin using ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Increased HIF-1 alpha expression is regulated by TSLPR inhibition & not IL-1 beta secretion (A) Human mDC were stimulated with SC glucan with either anti-TSLPR or IgG ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - miR-18b (miR-18b-5p) regulates Hif1 alpha & reduces apoptosis in mtNSC-34 cells. a Overexpressed miR-18b (miR-18b-5p) decreased Hif1 alpha & Mef2c proteins. Both Mctp1 ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Hypoxic conditions upregulate CNN3 in BeWo cells.BeWo cells were cultured either under normoxic or under hypoxic conditions for 24(A) Total protein lysates were examined ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Dynamics of the hypoxia-triggered decrease in MET phosphorylation & its reversal upon reoxygenation(A) MCF10A cells were incubated under normoxia or hypoxia for 1 h. They ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - The role of HIF-1 in purinosome formation.a, quantifying the number of purinosome-containing cells in normoxia or hypoxia (24 h) in purine-rich medium & normoxia in ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - C/EBPδ promotes HIF-1 alpha expression in macrophages through inhibition of FBXW7 alpha a) RT-PCR analysis of FBXW7 isoform expression from different sources as ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Hypoxia downregulates CFTR expression. (a) Phase-contrast photographs of MDCK cells treated with normoxia & hypoxia conditions; (b) Western blot showing downregulation of ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - NRP1 & MET on HT1080 cells are accessible for blood-borne rhodocetin-alpha beta (A) expression of NRP1 & MET by HT1080 cells was proven by flow cytometry. Gray, ...read more

Immunohistochemistry: HIF-1 alpha Antibody [NB100-449] - NRP1 & MET on HT1080 cells are accessible for blood-borne rhodocetin-alpha beta (A) expression of NRP1 & MET by HT1080 cells was proven by flow cytometry. Gray, ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Dectin-1-induced TSLP negatively regulates pro-IL-1 beta & HIF-1 alpha . (A) Human mDC were stimulated with SC glucan, CA glucan or heat killed C. albicans hyphae with ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - Involvement of MET in Akt & Erk pathway activation under hypoxiaMCF10A cells were transfected with a pool of three MET-targeting siRNAs (20 nM) or a control siRNA ...read more

Western Blot: HIF-1 alpha Antibody [NB100-449] - beta -Glucan size affects the requirement for reactive oxygen species (ROS) in IL-1 beta induction. (A) Human monocyte-derived dendritic cell (mDC) stimulated with ...read more

Product Details

Summary

Reactivity	Hu, Mu, Rt, Ca, Ch, Gt, Pm, Pm, BvSpecies Glossary
Applications	WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, ChIP, KD, KO
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	0.2 mg/ml

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HIF-1 alpha Antibody Summary

Immunogen	The immunogen recognized by this HIF-1 alpha Antibody maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1 [Uniprot# Q16665].
Predicted Species	Bovine (100%). Backed by our 100% Guarantee.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	HIF1A
Purity	Immunogen affinity purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions

Chromatin Immunoprecipitation (ChIP) 1:10-1:500
ELISA 1:100-1:2000
Flow (Intracellular)
Flow Cytometry 0.125 ug per 1 million cells in a 150 mcl reaction
Immunocytochemistry/ Immunofluorescence 1:10-1:500
Immunohistochemistry 1:100 - 1:500
Immunohistochemistry-Frozen 1:50-1:200
Immunohistochemistry-Paraffin 1:50-1:200
Immunoprecipitation 2-5 ug/mg lysate
Knockdown Validated
Knockout Validated
Simple Western 1:200
Western Blot 1:2000 - 1:10000

Application Notes

ChIP usage was reported in scientific literature (PMID: 25557133). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. For IHC-P, Tris-EDTA pH 9.0 buffer is recommended for the heat induced epitope retrieval. ELISA (PMID: 17556599 and 16966370). Knockout data (PMID: 31793879). Use in Flow-intracellular reported in scientific literature (PMID:31722203).

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Control

Human Brain Cerebral Cortex Whole Tissue Lysate (Adult Whole Normal)
Human Pancreas Whole Tissue Lysate (Adult Whole Tumor)
COS-7 Nuclear Hypoxic Induced Cell Lysate
HeLa CoCl2 treated/Untreated Cell Lysate
HepG2 CoCl2 treated/Untreated Cell Lysate
HeLa Hypoxic / Normoxic Cell Lysate
HepG2 Hypoxic / Normoxic Cell Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate

Reviewed Applications

Read 29 Reviews rated 4.3

using
NB100-449 in the following applications:

Western Blot
Immunohistochemistry-Paraffin
Immunoprecipitation
Immunofluorescence
Simple Western

Publications

Read Publications using
NB100-449 in the following applications:

Chemotaxis
4 publications
ELISA
2 publications
Flow Cytometry
1 publication
Flow Cytometry Control
1 publication
Functional Assay
1 publication
ICC/IF
10 publications
IF/IHC
21 publications
IHC
1 publication
IHC-Fr
8 publications
IHC-P
16 publications
IP
3 publications
Immunocytochemistry/ Immunofluorescence
1 publication
KD
1 publication
KO
1 publication
PLA
1 publication
WB
139 publications
Western Blot
3 publications

Reactivity Notes

Monkey (COS-7) and Rat reactivities were reported in customer review feedback. Detection of HIF1 alpha in both Mouse and Human tissue by IHC. Chicken reactivity was reported in scientific literature (PMID: 25632022). Canine reactivity reported in scientific literature (PMID: 28701694). Reactivity with Goat is reported in PMID: 21599540.

Packaging, Storage & Formulations

Storage	Store at 4C. Do not freeze.
Buffer	TBS, 0.1% BSA
Preservative	0.09% Sodium Azide
Concentration	0.2 mg/ml
Purity	Immunogen affinity purified

Alternate Names for HIF-1 alpha Antibody

AINT
anti-HIF-1 alpha
anti-HIF1A
ARNT interacting protein
ARNT-interacting protein
Basic-helix-loop-helix-PAS protein MOP1
BHLHE78
Class E basic helix-loop-helix protein 78
HIF 1A
HIF1 alpha
HIF-1 alpha
HIF1
HIF1A
HIF-1a
HIF-1alpha
HIF-1-alpha
HIF1-alpha
hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha
hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
hypoxia-inducible factor 1-alpha
Member of PAS protein 1
member of PAS superfamily 1
MOP1
PAS domain-containing protein 8
PASD8

Background

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).

Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of FoxP3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205

6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016

7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G., ... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIF-1 alpha Antibody (NB100-449)(300)

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Showing Publications 1 - 10 of 300. Show All 300 Publications.

Publications using NB100-449	Applications	Species
Scout Bowman-Gibson, Chandni Chandiramani, Madison L Stone, Christopher A Waker, Traci M Rackett, Rose A Maxwell, David N Dhanraj, Thomas L Brown Streamlined Analysis of Maternal Plasma Indicates Small Extracellular Vesicles are Significantly Elevated in Early-Onset Preeclampsia. Reproductive sciences (Thousand Oaks, Calif.) 2024-05-22 [PMID: 38777947]
Mojca Pavlin, Nives Škorja Milić, Maša Kandušer, Sergej Pirkmajer Importance of the electrophoresis and pulse energy for siRNA-mediated gene silencing by electroporation in differentiated primary human myotubes BioMedical Engineering OnLine 2024-05-16 [PMID: 38750477]
Milankumar Prajapati, Jared Z. Zhang, Lauren Chiu, Grace S. Chong, Courtney J. Mercadante, Heather L. Kowalski, Bradley Delaney, Jessica A. Anderson, Shuling Guo, Mariam Aghajan, Thomas B. Bartnikas Hepatic HIF2 is a key determinant of manganese excess and polycythemia in SLC30A10 deficiency JCI Insight 2024-04-23 [PMID: 38652538]
Divya T. Chemparathy, Sudipta Ray, Chase Ochs, Natasha Ferguson, Dinesh Y. Gawande, Shashank M. Dravid, Shannon Callen, Susmita Sil, Shilpa Buch Neuropathogenic role of astrocyte‐derived extracellular vesicles in HIV‐associated neurocognitive disorders Journal of Extracellular Vesicles 2024-04-22 [PMID: 38647111]
Ahmad Reza Panahi Meymandi, Behnia Akbari, Tahereh Soltantoyeh, Zahra Shahosseini, Mina Hosseini, Jamshid Hadjati, Hamid Reza Mirzaei PX-478, an HIF-1 alpha inhibitor, impairs mesoCAR T cell antitumor function in cervical cancer Frontiers in Oncology 2024-02-15 [PMID: 38425341]
Uttkarsh Ayyangar, Aneesh Karkhanis, Heather Tay, Aliya Farissa Binte Afandi, Oindrila Bhattacharjee, Lalitha KS, Sze Han Lee, James Chan, Srikala Raghavan Metabolic rewiring of macrophages by epidermal-derived lactate promotes sterile inflammation in the murine skin The EMBO Journal 2024-02-28 [PMID: 38418556]
Anusha Acharya, Fenghua Bian, Jose Gomez-Arroyo, Kimberly A. Wagner, Vladimir V. Kalinichenko, Tanya V. Kalin Hypoxia represses FOXF1 in lung endothelial cells through HIF-1 alpha Frontiers in Physiology 2024-01-11 [PMID: 38274049]
Gobelli D, Serrano-Lorenzo P, Esteban-Amo MJ et al. The mitochondrial succinate dehydrogenase complex controls the STAT3-IL-10 pathway in inflammatory macrophages iScience 2023-08-18 [PMID: 37575201]
Tsai HC, Tong ZJ, Hwang TL et al. Acrolein produced by glioma cells under hypoxia inhibits neutrophil AKT activity and suppresses anti-tumoral activities Free radical biology & medicine 2023-07-04 [PMID: 37414347] (IHC, Mouse)	IHC	Mouse
Romero-Lopez M, Oria M, Ferrer-Marquez F et al. Fetal lung hypoxia and energetic cell failure in the nitrofen-induced congenital diaphragmatic hernia rat model Pediatric surgery international 2023-04-13 [PMID: 37055635]
Saigusa H, Mimura I, Kurata Y et al. Hypoxia-inducible lncRNA MIR210HG promotes HIF1? expression by inhibiting miR-93-5p in renal tubular cells The FEBS journal 2023-04-08 [PMID: 37029581] (WB, Human) Details: 1:1000 dilution	WB	Human
Deval G, Nedder M, Degrelle S et al. Benzo(a)pyrene and Cerium Dioxide Nanoparticles in Co-Exposure Impair Human Trophoblast Cell Stress Signaling International journal of molecular sciences 2023-03-12 [PMID: 36982514] (IHC-P, Human) Details: Dilution: 1:500	IHC-P	Human
van Dam TP DNA polymerase beta prevents AID-instigated mutagenic non-canonical mismatch DNA repair bioRxiv 2020-01-01 (IP, Mouse)	IP	Mouse
Marrocco A Alterations of CCSP expression and macrophages metabolism in the development of silica-induced pulmonary inflammation and fibrosis Thesis 2020-01-01 (ICC/IF, Mouse)	ICC/IF	Mouse
Rashed FB, Kate WD, Fanta M et al. Influence of 2-Nitroimidazoles in the Response of FaDu Cells to Ionizing Radiation and Hypoxia/Reoxygenation Stress Antioxidants (Basel, Switzerland) 2023-02-06 [PMID: 36829948] (WB, Human)	WB	Human
Moreno-Loshuertos R, Movilla N, Marco-Brualla J et al. A Mutation in Mouse MT-ATP6 Gene Induces Respiration Defects and Opposed Effects on the Cell Tumorigenic Phenotype International Journal of Molecular Sciences 2023-01-09 [PMID: 36674816] (Flow Cytometry)	Flow Cytometry
Noonan ML, Ni P, Solis E et al. Osteocyte Egln1/Phd2 links oxygen sensing and biomineralization via FGF23 Bone Research 2023-01-18 [PMID: 36650133] (Western Blot)	Western Blot
Mehrabani M, Jafarinejad-Farsangi S, Raeiszadeh M et al. Effects of the Ethanol and Ethyl Acetate Extracts of Terminalia chebula Retz. on Proliferation, Migration, and HIF-1 alpha and CXCR-4 Expression in MCF-7 Cells: an In Vitro Study Applied biochemistry and biotechnology 2022-12-31 [PMID: 36585552]
Sun L, Kim S, Mori R et al. Astaxanthin Exerts Immunomodulatory Effect by Regulating SDH-HIF-1a Axis and Reprogramming Mitochondrial Metabolism in LPS-Stimulated RAW264.7 Cells Marine Drugs 2022-10-25 [PMID: 36354983] (WB)	WB
Ben-Cheikh A, Bali A, Guerfali FZ et al. Hypoxia-Inducible Factor-1 Alpha Stabilization in Human Macrophages during Leishmania major Infection Is Impaired by Parasite Virulence The Korean journal of parasitology 2022-10-01 [PMID: 36320108] (WB, Human) Details: Dilution used in WB 1:2000	WB	Human
Tiwari R, Bommi PV, Gao P et al. Chemical inhibition of oxygen-sensing prolyl hydroxylases impairs angiogenic competence of human vascular endothelium through metabolic reprogramming iScience 2022-10-21 [PMID: 36157579] (Functional Assay)	Functional Assay
Ye C, Li H, Li Y et al. Hypoxia-induced HMGB1 promotes glioma stem cells self-renewal and tumorigenicity via RAGE iScience 2022-09-16 [PMID: 36034219] (KD, IHC-Fr, WB, IHC-P, Human)	KD, IHC-Fr, WB, IHC-P	Human
Tournaire G, Loopmans S, Stegen S et al. Skeletal progenitors preserve proliferation and self-renewal upon inhibition of mitochondrial respiration by rerouting the TCA cycle Cell reports 2022-07-26 [PMID: 35905715] (WB, Mouse)	WB	Mouse
Vo VTA, Kim S, Hua TNM et al. Iron commensalism of mesenchymal glioblastoma promotes ferroptosis susceptibility upon dopamine treatment Communications biology 2022-06-16 [PMID: 35710828] (WB, Mouse)	WB	Mouse
Han JS, Jeon YG, Oh M et al. Adipocyte HIF2 alpha functions as a thermostat via PKA C alpha regulation in beige adipocytes Nature communications 2022-06-07 [PMID: 35672324] (IF/IHC, Mouse)	IF/IHC	Mouse
Mennerich D, Kubaichuk K, Raza GS et al. ER-stress promotes VHL-independent degradation of hypoxia-inducible factors via FBXW1A/ beta TrCP Redox biology 2022-01-18 [PMID: 35074541] (WB, Human, Mouse)	WB	Human, Mouse
Craps J, Joris V, Baldeschi L et al. miR-199a Downregulation as a Driver of the NOX4/HIF-1 alpha/VEGF-A Pathway in Thyroid and Orbital Adipose Tissues from Graves Patients International Journal of Molecular Sciences 2021-12-23 [PMID: 35008579] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Leinhos L Myofibroblast differentiation in hypoxia: a novel role for ArhGAP29 Thesis (WB)	WB
Rashed F, Diaz-Dussan D, Mashayekhi F et al. Cellular mechanism of action of 2-nitroimidzoles as hypoxia-selective therapeutic agents Redox Biology 2022-03-01 [PMID: 35430547] (WB, Human)	WB	Human
Mulangala J, Akers EJ, Solly EL et al. Pro-Calcific Environment Impairs Ischaemia-Driven Angiogenesis International journal of molecular sciences 2022-03-20 [PMID: 35328786] (WB, Human)	WB	Human
Andreas M. Bapst, Thomas Knöpfel, Karen A. Nolan, Faik Imeri, Claus D. Schuh, Andrew M. Hall, Jia Guo, Dörthe M. Katschinski, Roland H. Wenger Neurogenic and pericytic plasticity of conditionally immortalized cells derived from renal erythropoietin‐producing cells Journal of Cellular Physiology 2022-01-10 [PMID: 35014036]
AkusjArvi SS, Ambikan AT, Krishnan S Et al. Integrative proteo-transcriptomic and immunophenotyping signatures of HIV-1 elite control phenotype: A cross-talk between glycolysis and HIF signaling iScience 2022-01-10 [PMID: 35005552] (ICC/IF, Human) Details: Citation using the Alexa Fluor 594 version of this antibody.	ICC/IF	Human
Mallet C, Cochard J, Leclercq S et al. Hypoxia and HIF-1 Trigger Marek's Disease Virus Reactivation in Lymphoma-Derived Latently Infected T Lymphocytes Journal of virology 2021-12-22 [PMID: 34936483]
Williams NC, Ryan DG, Costa ASH et al. Signalling metabolite L-2-hydroxyglutarate activates the transcription factor HIF-1alpha in lipopolysaccharide-activated macrophages The Journal of biological chemistry 2021-12-17 [PMID: 34929172] (WB, Human)	WB	Human
Janbandhu V, Tallapragada V, Patrick R Et al. Hif-1a suppresses ROS-induced proliferation of cardiac fibroblasts following myocardial infarction Cell stem cell 2021-11-05 [PMID: 34762860]
Sheshadri N, Poria DK, Sharan S Et al. PERK signaling through C/EBP delta contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells Cell death & disease 2021-11-01 [PMID: 34725321] (WB, Human)	WB	Human
Shih HM, Pan SY, Wu CJ Et al. Transforming growth factor- beta 1 decreases erythropoietin production through repressing hypoxia-inducible factor 2 alpha in erythropoietin-producing cells Journal of biomedical science 2021-11-02 [PMID: 34724959] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Willenborg S, Sanin DE, Jais A Et al. Mitochondrial metabolism coordinates stage-specific repair processes in macrophages during wound healing Cell metabolism 2021-10-25 [PMID: 34715039] (WB, Mouse)	WB	Mouse
Nortunen M, Parkkila S, Saarnio J Et al. Carbonic Anhydrases II and IX in Non-ampullary Duodenal Adenomas and Adenocarcinoma The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2021-11-01 [PMID: 34636283] (IF/IHC)	IF/IHC
Sil S, Singh S, Chemparathy DT et al. Astrocytes & Astrocyte derived Extracellular Vesicles in Morphine Induced Amyloidopathy: Implications for Cognitive Deficits in Opiate Abusers Aging and disease 2021-09-01 [PMID: 34527417]
Wang K, Reichermeier KM, Liu X Quantitative analyses for effects of neddylation on CRL2VHL substrate ubiquitination and degradation Protein science : a publication of the Protein Society 2021-08-30 [PMID: 34459035]
�korja Mili? N, Dolinar K, Mi� K et al. Suppression of Pyruvate Dehydrogenase Kinase by Dichloroacetate in Cancer and Skeletal Muscle Cells Is Isoform Specific and Partially Independent of HIF-1? International Journal of Molecular Sciences 2021-08-10 [PMID: 34445316] (Western Blot)	Western Blot
Marrocco A, Frawley K, Pearce LL et al. Metabolic Adaptation of Macrophages as Mechanism of Defense against Crystalline Silica The Journal of Immunology 2021-09-15 [PMID: 34433619] (Immunocytochemistry/ Immunofluorescence)	Immunocytochemistry/ Immunofluorescence
Imbroisi Filho R, Ochioni AC, Esteves AM et al. Western diet leads to aging-related tumorigenesis via activation of the inflammatory, UPR, and EMT pathways Cell death & disease 2021-06-23 [PMID: 34162829] (WB, Mouse)	WB	Mouse
Thirunavukkarasu M, Tipu Rishi M, Pradeep SR et al. Heat shock protein A12B gene therapy improves perfusion, promotes neovascularization, and decreases fibrosis in a murine model of hind limb ischemia Surgery 2021-06-03 [PMID: 34092373]
Prieto-Fernandez E, Egia-Mendikute L, Vila-Vecilla L, et al. Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2 and heparan sulfate Emerg Microbes Infect 2021-05-20 [PMID: 34013835]
Laquatra C, Sanchez-Martin C, Dinarello A et al. HIF1?-dependent induction of the mitochondrial chaperone TRAP1 regulates bioenergetic adaptations to hypoxia Cell Death & Disease 2021-05-01 [PMID: 33934112]
van Kuijk K, Demandt JAF, Perales-PatOn J et al. DEFICIENCY OF MYELOID PHD PROTEINS AGGRAVATES ATHEROGENESIS VIA MACROPHAGE APOPTOSIS AND PARACRINE FIBROTIC SIGNALING: Atherogenic effects of myeloid PHD knockdown Cardiovascular research 2021-04-26 [PMID: 33913468]
Rashed FB, Stoica AC, MacDonald D et al. Identification of proteins and cellular pathways targeted by 2-nitroimidazole hypoxic cytotoxins Redox biology 2021-02-21 [PMID: 33640700] (WB, Human)	WB	Human
Noonan ML, Ni P, Agoro R et al. The HIF-PHI BAY 85-3934 (Molidustat) improves anemia and is associated with reduced levels of circulating FGF23 in a CKD mouse model Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2021-02-16 [PMID: 33592127]
Ito E, Sato Y, Kobayashi T, et al. Treatment with an active vitamin D analogue blocks hypothalamic dysfunction-induced bone loss in mice Biochemical and biophysical research communications 2021-01-21 [PMID: 33486191] (IF/IHC, Mouse)	IF/IHC	Mouse
Torretta S, Scagliola A, Ricci L et al. D-mannose suppresses macrophage IL-1 beta production Nature communications 2020-12-11 [PMID: 33311467] (WB, Mouse)	WB	Mouse
Dadgar S, Troncoso JR, Siegel ER et al. Spectroscopic investigation of radiation-induced reoxygenation in radiation-resistant tumors Neoplasia 2020-11-18 [PMID: 33220616] (IHC-Fr, Mouse) Details: Frozen immunohistochemical analysis performed on mice tumors treated with radiation	IHC-Fr	Mouse
Zandona, A, Lihtar, G Et al. Vitamin B3-Based Biologically Active Compounds as Inhibitors of Human Cholinesterases. Int J Mol Sci 2020-10-29 [PMID: 33138280] (IHC-P, Mouse)	IHC-P	Mouse
Gauthier LR, Saati M, Bensalah-Pigeon H et al. The HIF1 alpha/JMY pathway promotes glioblastoma stem-like cell invasiveness after irradiation Sci Rep 2020-10-30 [PMID: 33128011] (IF/IHC, Mouse)	IF/IHC	Mouse
Prieto-Fern�ndez E, Egia-Mendikute L, Bosch A et al. Hypoxia Promotes Syndecan-3 Expression in the Tumor Microenvironment Frontiers in Immunology 2020-09-30 [PMID: 33117401] (Western Blot)	Western Blot
Pirkmajer S, Bezjak K, Matkovi? U et al. Ouabain Suppresses IL-6/STAT3 Signaling and Promotes Cytokine Secretion in Cultured Skeletal Muscle Cells Frontiers in Physiology 2020-09-25 [PMID: 33101052]
Riou R, Ladli M, Gerbal-Chaloin S et al. ARID1A loss in adult hepatocytes activates beta-catenin-mediated erythropoietin transcription Elife 2020-10-21 [PMID: 33084574] (Mouse)		Mouse
Dyah Ari Nugrahaningrum, Olivia Marcelina, Caiping Liu, Shourong Wu, Vivi Kasim Dapagliflozin Promotes Neovascularization by Improving Paracrine Function of Skeletal Muscle Cells in Diabetic Hindlimb Ischemia Mice Through PHD2/HIF-1 alpha Axis Frontiers in Pharmacology 2020-08-10 [PMID: 32848736]
Megan E. Vaughan, Martina Wallace, Michal K. Handzlik, Alanna B. Chan, Christian M. Metallo, Katja A. Lamia Cryptochromes Suppress HIF1 alpha in Muscles iScience 2020-07-03 [PMID: 32683313]
Kim KY, Kim YR, Choi KW et al. Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients Transl Neurodegener 2020-07-01 [PMID: 32605607]
Ghosh P, Guo Y, Ashrafi A et al. Oxygen-enhanced optoacoustic tomography reveals the effectiveness of targeting heme and oxidative phosphorylation at normalizing tumor vascular oxygenation Cancer Res. 2020-06-16 [PMID: 32546631] (IF/IHC, Human)	IF/IHC	Human
Hu L, Chen X, Qiu S et al. Intra-Pancreatic Insulin Nourishes Cancer Cells: Do Insulin-Receptor Antagonists such as PGG and EGCG Play a Role? Am. J. Chin. Med. 2020-05-28 [PMID: 32468825]
Sil S, Hu G, Liao K et al. HIV-1 Tat-mediated astrocytic amyloidosis involves the HIF-1 alpha/lncRNA BACE1-AS axis PLoS Biol. 2020-05-26 [PMID: 32453744] (ICC/IF, Human)	ICC/IF	Human
Doigneaux C, Pedley AM, Mistry IN et al. Hypoxia Drives the Assembly of the Multi-Enzyme Purinosome Complex J. Biol. Chem. 2020-05-21 [PMID: 32439803] (WB, Human)	WB	Human
Alexander R K, Liou Y H et al. Bmal1 integrates mitochondrial metabolism and macrophage activation. Elife 2020-12-05 [PMID: 32396064] (WB, Mouse)	WB	Mouse
Yamazaki H, Tanaka T, Mie K et al. Assessment of postoperative adjuvant treatment using toceranib phosphate against adenocarcinoma in dogs J. Vet. Intern. Med. 2020-04-08 [PMID: 32267594] (IHC-P, Canine)	IHC-P	Canine
Das Gupta K, Shakespear MR, Curson JEB et al. Class IIa Histone Deacetylases Drive Toll-like Receptor-Inducible Glycolysis and Macrophage Inflammatory Responses via Pyruvate Kinase M2 Cell Rep 2020-02-25 [PMID: 32101747] (WB, Human)	WB	Human
Sugahara M, Tanaka S, Tanaka T et al. Prolyl Hydroxylase Domain Inhibitor Protects against Metabolic Disorders and Associated Kidney Disease in Obese Type 2 Diabetic Mice J. Am. Soc. Nephrol. 2020-01-29 [PMID: 31996409]
Sola-Penna M, PaixAo LP, Branco JR, et al. Serotonin activates glycolysis and mitochondria biogenesis in human breast cancer cells through activation of the Jak1/STAT3/ERK1/2 and adenylate cyclase/PKA, respectively Br. J. Cancer 2019-12-10 [PMID: 31819176] (WB, Human)	WB	Human
Yambire KF, Rostosky C, Watanabe T Impaired lysosomal acidification triggers iron deficiency and inflammation in vivo Elife 2019-12-03 [PMID: 31793879] (WB, KO, Mouse)	WB, KO	Mouse
Di Gioia M, Spreafico R, Springstead JR et al. Endogenous oxidized phospholipids reprogram cellular metabolism and boost hyperinflammation Nat. Immunol. 2019-11-25 [PMID: 31768073] (WB, Mouse)	WB	Mouse
Baumeister J, Chatain N, Hubrich A et al. Hypoxia-inducible factor 1 (HIF-1) is a new therapeutic target in JAK2V617F-positive myeloproliferative neoplasms Leukemia 2020-04-01 [PMID: 31728053]
Gupta SS, Sharp R, Hofferek C et al. NIX-Mediated Mitophagy Promotes Effector Memory Formation in Antigen-Specific CD8+ T Cells Cell Rep 2019-11-12 [PMID: 31722203] (Flow Cytometry Control, Mouse)	Flow Cytometry Control	Mouse
Morikawa M, Mitani Y, Holmborn K et al. The ALK-1/SMAD/ATOH8 axis attenuates hypoxic responses and protects against the development of pulmonary arterial hypertension Sci Signal 2019-11-12 [PMID: 31719172]
Lin HY, Chou PC, Chen YH et al. Dietary Supplementation of 25-Hydroxycholecalciferol Improves Livability in Broiler Breeder Hens-Amelioration of Cardiac Pathogenesis and Hepatopathology Animals (Basel) 2019-10-08 [PMID: 31597394] (WB, Chicken)	WB	Chicken
Nasyrov E, Nolan KA, Wenger RH et al. The neuronal oxygen-sensing pathway controls postnatal vascularization of the murine brain FASEB J. 2019-08-30 [PMID: 31469589]
Bailey JD, Diotallevi M, Nicol T, et al. Nitric Oxide Modulates Metabolic Remodeling in Inflammatory Macrophages through TCA Cycle Regulation and Itaconate Accumulation Cell Rep 2019-07-02 [PMID: 31269442] (WB)	WB
Bahrami B, Shen W, Zhu L et al. Effects of VEGF inhibitors on human retinal pigment epithelium under high glucose and hypoxia Clin. Experiment. Ophthalmol. 2019-07-02 [PMID: 31265210]
Baik SH, Kang S, Lee W et al. A Breakdown in Metabolic Reprogramming Causes Microglia Dysfunction in Alzheimer's Disease Cell Metab. 2019-06-24 [PMID: 31257151]
Chowdhury D, Alrefai H, Landero Figueroa JA et al. Metallothionein 3 Controls the Phenotype and Metabolic Programming of Alternatively Activated Macrophages Cell Rep 2019-06-25 [PMID: 31242420] (ICC/IF, Mouse)	ICC/IF	Mouse
Chen Y, Zhao B, Zhu Y et al. HIF-1-VEGF-Notch mediates angiogenesis in temporomandibular joint osteoarthritis Am J Transl Res. 2019-05-15 [PMID: 31217867] (WB, IF/IHC, Rat)	WB, IF/IHC	Rat
Elder MJ, Webster SJ, Fitzmaurice TJ et al. Dendritic Cell-Derived TSLP Negatively Regulates HIF-1 alpha and IL-1 beta During Dectin-1 Signaling Front Immunol 2020-05-08 [PMID: 31139177] (WB, Human)	WB	Human
Igari K, Kelly MJ, Yamanouchi D Digoxin Attenuates Receptor Activation of NF-kappa B Ligand-Induced Osteoclastogenesis in Macrophages J. Vasc. Res. 2019-05-14 [PMID: 31085912] (WB, Mouse)	WB	Mouse
Duan LJ, Fong GH. Developmental vascular pruning in neonatal mouse retinas is programmed in the astrocytic oxygen sensing mechanism Development 2019-03-25 [PMID: 30910827] (WB, Mouse)	WB	Mouse
Grigelioniene G, Suzuki HI, Taylan F et al. Gain-of-function mutation of microRNA-140 in human skeletal dysplasia Nat. Med. 2019-02-25 [PMID: 30804514] (WB, Mouse)	WB	Mouse
Li HS, Zhou YN, Li L et al. HIF-1a protects against oxidative stress by directly targeting mitochondria. Redox Biol 2019-01-14 [PMID: 30686776] (IHC-P, IP, Human)	IHC-P, IP	Human
Krueger K, Catanese L, Sciesielski LK et al. Deletion of an intronic HIF-2a binding site suppresses hypoxia-induced WT1 expression. Biochim Biophys Acta Gene Regul Mech. 2018-11-20 [PMID: 30468780] (Chemotaxis, Human)	Chemotaxis	Human
Bae YH, Joo H, Bae J et al. Brain injury induces HIF-1a-dependent transcriptional activation of LRRK2 that exacerbates brain damage. Cell Death Dis. 2018-11-12 [PMID: 30420654] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Valencia-Cervantes J, Huerta-Yepez S, Aquino-Jarquin G et al. Hypoxia increases chemoresistance in human medulloblastoma DAOY cells via hypoxia‑inducible factor 1a‑mediated downregulation of the CYP2B6, CYP3A4 and CYP3A5 enzymes and inhibition of cell proliferation. Oncol. Rep. 2018-10-12 [PMID: 30320358] (Human)		Human
Fauzia E, Barbhuyan Tk, Shrivastava Ak et al. Chick Embryo: A Preclinical Model for Understanding Ischemia-Reperfusion Mechanism. Front Pharmacol. 2018-09-21 [PMID: 30298003] (WB, Chicken)	WB	Chicken
Mouly L, Mamouni K, Gence R et al. PARP-1-dependent RND1 transcription induced by topoisomerase I cleavage complexes confers cellular resistance to camptothecin. Cell Death Dis 2018-09-12 [PMID: 30209297] (WB, Human)	WB	Human
Koenis DS, Medzikovic L, van Loenen PB et al. Nuclear Receptor Nur77 Limits the Macrophage Inflammatory Response through Transcriptional Reprogramming of Mitochondrial Metabolism Cell Rep 2018-08-21 [PMID: 30134173] (WB, Mouse)	WB	Mouse
Koenis D, Medzikovic L, Vos M et al. Nur77 variants solely comprising the amino-terminal domain activate hypoxia-inducible factor-1a and affect bone marrow homeostasis in mouse and man J. Biol. Chem. 2018-08-15 [PMID: 30111591] (WB, Mouse)	WB	Mouse
kim YE, Lee M, Gu H et al. HIF-1a activation in myeloid cells accelerates dextran sodium sulfate-induced colitis progression in mice. Dis Model Mech. 2018-07-30 [PMID: 29967068] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Li B, Zhu Y, Sun Q et al. Reversal of the Warburg effect with DCA in PDGF-treated human PASMC is potentiated by pyruvate dehydrogenase kinase-1 inhibition mediated through blocking Akt/GSK-3 beta signalling Int J Mol Med. 2018-06-26 [PMID: 29956736] (WB, Human)	WB	Human
Mekki MS, Mougel A, Vinchent A et al. Hypoxia leads to decreased autophosphorylation of the MET receptor but promotes its resistance to tyrosine kinase inhibitors Oncotarget 2018-06-05 [PMID: 29930749] (WB, Human)	WB	Human
Hamann I, Krys D, Glubrecht D et al. Expression and function of hexose transporters GLUT1, GLUT2, and GLUT5 in breast cancer-effects of hypoxia The FASEB Journal 2018-04-13 [PMID: 29913554] (IF/IHC, Human)	IF/IHC	Human
Niland S, Komljenovic D, Macas J et al. Rhodocetin-aB selectively breaks the endothelial barrier of the tumor vasculature in HT1080 fibrosarcoma and A431 epidermoid carcinoma tumor models Oncotarget 2018-04-27 [PMID: 29854288] (WB)	WB
Fukushima A, Zhang L, Huqi A et al. Acetylation contributes to hypertrophy-caused maturational delay of cardiac energy metabolism JCI Insight 2018-05-17 [PMID: 29769443] (WB, Human)	WB	Human
Li HS, Zhou YN, Li L et al. WITHDRAWN:Mitochondrial targeting of HIF-1alpha inhibits hypoxia-induced apoptosis independently of its transcriptional activity. Free Radic Biol Med 2018 Apr 26 [PMID: 29704620]
Ferecatu I, Canal F, Fabbri L et al. Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1a activation. PLoS ONE 2018-03-29 [PMID: 29596470] (WB, Human)	WB	Human
Cho JH, Kim GY, Mansfield BC, Chou JY. Hepatic glucose-6-phosphatase-A deficiency leads to metabolic reprogramming in glycogen storage disease type Ia. Biochem. Biophys. Res. Commun. 2018-04-15 [PMID: 29545180] (Mouse)		Mouse
Bok S, Kim YE, Woo Y et al. Hypoxia-inducible factor-1a regulates microglial functions affecting neuronal survival in the acute phase of ischemic stroke in mice Oncotarget 2017-12-19 [PMID: 29340071] (IHC-Fr, WB, Mouse)	IHC-Fr, WB	Mouse
Geoghegan F, Buckland RJ, Rogers E et al. Bioenergetics of acquired cisplatin resistant H1299 non-small cell lung cancer and P31 mesothelioma cells Oncotarget. 2017-10-16 [PMID: 29212260] (Human)		Human
Almiron Bonnin DA, Havrda MC, Lee MC et al. Secretion-mediated STAT3 activation promotes self-renewal of glioma stem-like cells during hypoxia Oncogene. 2017-11-20 [PMID: 29155422] (WB, Mouse)	WB	Mouse
Palazon A, Tyrakis PA, Macias D et al. An HIF-1 alpha/VEGF-A Axis in Cytotoxic T Cells Regulates Tumor Progression Cancer Cell 2017-11-13 [PMID: 29136509] (WB, Mouse)	WB	Mouse
Palsson-McDermott EM, Dyck L, Zastona Z et al. Pyruvate Kinase M2 Is Required for the Expression of the Immune Checkpoint PD-L1 in Immune Cells and Tumors. Front Immunol. 2017-10-30 [PMID: 29081778] (WB, Mouse)	WB	Mouse
Lee S, Kim JH, Lee JH et al. Imaging Monitoring of Kupffer Cell Function and Hepatic Oxygen Saturation in Preneoplastic Changes During Cholangiocarcinogenesis Sci Rep. 2017-10-27 [PMID: 29079853] (WB, Mouse)	WB	Mouse
Domingo-Gonzalez R, Das S, Griffiths KL et al. Interleukin-17 limits hypoxia-inducible factor 1a and development of hypoxic granulomas during tuberculosis. JCI Insight. 2017-10-05 [PMID: 28978810] (WB, Mouse) Details: HIF1 alpha antibody used to evaluate the effect of IL-17 on the shift to glycolysis and the development of hypoxic tuberculosis granulomas	WB	Mouse
Okadome J, Matsumoto T, Yoshiya K et al. BubR1 insufficiency impairs angiogenesis in aging and in experimental critical limb ischemic mice. J. Vasc. Surg. 2017-09-30 [PMID: 28974341]
Goodwin J, Choi H, Hsieh MH et al. Targeting HIF-1a/PDK1 Axis by Dichloroacetate (DCA) Suppresses Bleomycin-induced Pulmonary Fibrosis Am. J. Respir. Cell Mol. Biol. 2017-09-15 [PMID: 28915065] (Mouse)		Mouse
Huang R, Hayashi Y, Yan X et al HIF1A is a critical downstream mediator for hemophagocytic lymphohistiocytosis. Haematologica. 2017-11-01 [PMID: 28860338] (WB, Mouse) Details: Citation using the DyLight 405 version of this antibody.	WB	Mouse
MacLauchlan SC, Calabro NE, Huang Y et al. HIF-1alpha represses the expression of the angiogenesis inhibitor thrombospondin-2 Matrix Biol. 2017-08-05 [PMID: 28789925] (WB, Mouse)	WB	Mouse
Lee S, Kim JH, Lee JH et al. Non-invasive monitoring of the therapeutic response in sorafenib-treated hepatocellular carcinoma based on photoacoustic imaging. Eur Radiol 2017-07-27 [PMID: 28752217] (ICC/IF, Mouse)	ICC/IF	Mouse
Elder MJ, Webster SJ, Chee R et al. B-Glucan Size Controls Dectin-1-Mediated Immune Responses in Human Dendritic Cells by Regulating IL-1B Production Front Immunol 2017-07-24 [PMID: 28736555] (WB, Human)	WB	Human
Tavernier SJ, Vanlangenakker N, Vetters J et al. Opposing regulation and roles for PHD3 in lung dendritic cells and alveolar macrophages J. Leukoc. Biol. 2017-07-17 [PMID: 28716863] (WB)	WB
Zhang JT, Wang Y, Chen JJ et al. Defective CFTR leads to aberrant B-catenin activation and kidney fibrosis Sci Rep 2017-07-12 [PMID: 28701694] (WB, Canine)	WB	Canine
Papathanassiu AE, Ko JH, Imprialou M et al. BCAT1 controls metabolic reprogramming in activated human macrophages and is associated with inflammatory diseases Nat Commun. 2017-07-11 [PMID: 28699638] (WB, Mouse)	WB	Mouse
Carlessi R, Chen Y, Rowlands J et al. GLP-1 receptor signalling promotes B-cell glucose metabolism via mTOR-dependent HIF-1alpha activation. Sci Rep. 2017-06-01 [PMID: 28572610] (WB, Mouse)	WB	Mouse
Goodwin J, Neugent ML, Lee SY et al. The distinct metabolic phenotype of lung squamous cell carcinoma defines selective vulnerability to glycolytic inhibition. Nat Commun. 2017-05-26 [PMID: 28548087] (IHC-P, Human)	IHC-P	Human
Feigenson M, Eliseev RA, Jonason JH et al. PGE2 Receptor Subtype 1 (EP1) Regulates Mesenchymal Stromal Cell Osteogenic Differentiation by Modulating Cellular Energy Metabolism. J. Cell. Biochem. 2017-04-26 [PMID: 28444901] (WB, Mouse)	WB	Mouse
Kowalski GM, Kraakman MJ, Mason SA et al. Resolution of glucose intolerance in long-term high-fat, high-sucrose-fed mice. J. Endocrinol. 2017-06-01 [PMID: 28360081] (WB, Mouse)	WB	Mouse
Evans Colin E, Palazon Asis, Sim Jingwei et al. Modelling pulmonary microthrombosis coupled to metastasis: distinct effects of thrombogenesis on tumorigenesis. Biology Open 2017-01-01 [PMID: 28302670] (IF/IHC, Mouse)	IF/IHC	Mouse
Shen W, Yau B, Lee SR et al. Effects of Ranibizumab and Aflibercept on Human Muller Cells and Photoreceptors under Stress Conditions. Int J Mol Sci. 2017-03-01 [PMID: 28257068] (WB, Human)	WB	Human
Gardner PJ, Liyanage SE, Cristante E et al. Hypoxia inducible factors are dispensable for myeloid cell migration into the inflamed mouse eye Sci Rep 2017-01-23 [PMID: 28112274] (WB, Mouse)	WB	Mouse
Mistry I, Tavassoli A Reprogramming the Transcriptional Response to Hypoxia with a Chromosomally Encoded Cyclic Peptide HIF-1 Inhibitor. ACS Synth Biol 2017-03-17 [PMID: 27978620] (PLA, Human)	PLA	Human
McGettrick AF, Corcoran SE, Barry PJ et al. Trypanosoma brucei metabolite indolepyruvate decreases HIF-1alpha and glycolysis in macrophages as a mechanism of innate immune evasion. Proc. Natl. Acad. Sci. U.S.A. 2016-11-29 [PMID: 27856732]
Wang Y, Wang H, Li J et al. Direct visualization of the phenotype of hypoxic tumor cells at single cell resolution in vivo using a new hypoxia probe. Intravital. 2016-10-28 [PMID: 27790387] (Human, Mouse)		Human, Mouse
Zhang H Regulation of Cartilage Tumors by Mutations in Isocitrate Dehydrogenases Thesis 1905-07-13
Mills El, Kelly B, Logan A et al. Succinate Dehydrogenase Supports Metabolic Repurposing of Mitochondria to Drive Inflammatory Macrophages. Cell 2016-09-21 [PMID: 27667687]
Alonso J, Galan M, Marti-Pamies I et al. NOR-1/NR4A3 regulates the cellular inhibitor of apoptosis 2 (cIAP2) in vascular cells: role in the survival response to hypoxic stress. Sci Rep 2016-09-22 [PMID: 27654514] (WB)	WB
Zhang H Regulation of Cartilage Tumors by Mutations in Isocitrate Dehydrogenases Thesis Jul 13 1905
Turlejski T, Humoud I, Desai R et al. Immunohistochemical evidence of tissue hypoxia and astrogliosis in the rostral ventrolateral medulla of spontaneously hypertensive rats. Brain Res. 2016-09-08 [PMID: 27616338] (IF/IHC)	IF/IHC
Prakash H, Nadella V, Singh S, Schmitz-Winnenthal H. CD14/TLR4 priming potentially recalibrates and exerts anti-tumor efficacy in tumor associated macrophages in a mouse model of pancreatic carcinoma. Sci Rep. 2016-08-11 [PMID: 27511884]
Lampropoulou V, Sergushichev A, Bambouskova M et al. Itaconate Links Inhibition of Succinate Dehydrogenase with Macrophage Metabolic Remodeling and Regulation of Inflammation Cell Metab. 2016-07-12 [PMID: 27374498] (WB, Mouse)	WB	Mouse
Mirzamohammadi F, Papaioannou G, Inloes JB et al. Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-b signalling. Nat Commun 2016-06-22 [PMID: 27329220] (WB, Mouse)	WB	Mouse
Pei L, Solis G, Nguyen MT et al. Paracellular epithelial sodium transport maximizes energy efficiency in the kidney J. Clin. Invest 2016-07-01 [PMID: 27214555] (WB, Mouse)	WB	Mouse
Semba H, Takeda N, Isagawa T et al. HIF-1alpha-PDk1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity. Nat Commun. 2016-05-18 [PMID: 27189088] (WB, Mouse)	WB	Mouse
Tu DG, Chang WW, Lin ST et al. Salmonella inhibits tumor angiogenesis by downregulation of vascular endothelial growth factor. Oncotarget. 2016-06-21 [PMID: 27175584] (WB, Mouse)	WB	Mouse
Kobayashi H, Liu Q, Binns TC et al. Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin. J. Clin. Invest. 2016-05-02 [PMID: 27088801] (WB, Mouse)	WB	Mouse
Nusken E, Wohlfarth M, Lippach G et al. Reduced Perinatal Leptin Availability May Contribute to Adverse Metabolic Programming in a Rat Model of Uteroplacental Insufficiency. Endocrinology. 2016-05-01 [PMID: 27007072] Details: This citation used the HRP version of this antibody.
Kurihara Toshihide, Westenskow Peter D, Gantner Marin L et al. Hypoxia-induced metabolic stress in retinal pigment epithelial cells is sufficient to induce photoreceptor degeneRation. ELife 2016-01-01 [PMID: 26978795] (IF/IHC, Mouse)	IF/IHC	Mouse
Shukla PK, Chaudhry KK, Mir H et al. Chronic ethanol feeding promotes azoxymethane and dextran sulfate sodium-induced colonic tumorigenesis potentially by enhancing mucosal inflammation BMC Cancer 2016-03-08 [PMID: 26951793] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Farsijani NM, Liu Q, Kobayashi H et al. Renal epithelium regulates erythropoiesis via HIF-dependent suppression of erythropoietin. J Clin Invest 2016-04-01 [PMID: 26927670]
Chapple SJ, Keeley TP, Mastronicola D et al. Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels. Free Radic. Biol. Med. 2015-12-15 [PMID: 26698668] (WB, Human)	WB	Human
Shum LC, White NS, Mills BN et al. Energy Metabolism in Mesenchymal Stem Cells During Osteogenic Differentiation. Stem Cells Dev 2016-01-15 [PMID: 26487485]
Kuei-Yang Hsiao, Meng-Hsing Wu, Ning Chang, Shang-Hsun Yang, Chun-Wei Wu, H Sunny Sun, Shaw-Jenq Tsai Coordination of AUF1 and miR-148a destabilizes DNA methyltransferase 1 mRNA under hypoxia in endometriosis. Molecular human reproduction 2016-09-20 [PMID: 26433194]
Konzack A, Jakupovic M, Kubaichuk K et al. Mitochondrial Dysfunction Due to Lack of Manganese Superoxide Dismutase Promotes Hepatocarcinogenesis. Antioxid Redox Signal 2015-11-10 [PMID: 26422659] (IF/IHC)	IF/IHC
Lojk J, Mis K, Pirkmajer S, Pavlin M. siRNA delivery into cultured primary human myoblasts - optimization of electroporation parameters and theoretical analysis. Bioelectromagnetics 2015-09-21 [PMID: 26388450] (WB, Human)	WB	Human
Niebler S, Angele P, Kujat R, Bosserhoff AK. Hypoxia-Inducible Factor 1 Is an Inductor of Transcription Factor Activating Protein 2 Epsilon Expression during Chondrogenic Differentiation. Biomed Res Int. 2015-08-14 [PMID: 26273614] (WB, Mouse) Details: HIF-1 alpha antibody was used for WB on lysates of murine multipotent mesenchymal stem cells (Figure 4a) and SW1353 cells treated or not with siRNA against HIF1 alpha (Figure 4c).	WB	Mouse
Miao H, Chen L, Hao L et al. Stearic acid induces proinflammatory cytokine production partly through activation of lactate-HIF1alpha pathway in chondrocytes. Sci Rep. 2015-08-14 [PMID: 26271607] (WB, Mouse)	WB	Mouse
Le Moan N, Baeten KM, Rafalski VA et al. Hypoxia Inducible Factor-1 in Astrocytes and/or Myeloid Cells Is Not Required for the Development of Autoimmune Demyelinating Disease eNeuro 2015-03-01 [PMID: 26213713] (IF/IHC)	IF/IHC
Garhyan J, Bhuyan S, Pulu I et al. Preclinical and Clinical Evidence of Mycobacterium tuberculosis Persistence in the Hypoxic Niche of Bone Marrow Mesenchymal Stem Cells after Therapy. Am J Pathol 2015-07-01 [PMID: 26066709]
Fan D, Coughlin LA, Neubauer MM et al. Activation of HIF-1alpha and LL-37 by commensal bacteria inhibits Candida albicans colonization. Nat Med 2015-07-01 [PMID: 26053625]
Kambayashi S, Igase M, Kobayashi K et al. Hypoxia inducible factor 1alpha expression and effects of its inhibitors in canine lymphoma. J Vet Med Sci 2015-11-01 [PMID: 26050843] (WB)	WB
Qiao S, Dennis M, Song X et al. A REDD1/TXNIP pro-oxidant complex regulates ATG4B activity to control stress-induced autophagy and sustain exercise capacity Nat Commun 2015-04-28 [PMID: 25916556] (WB, Human)	WB	Human
Hribar K C, Finlay D, Ma X. et al. et al. Nonlinear 3D projection printing of concave hydrogel microstructures for long-term multicellular spheroid and embryoid body culture. Lab on a Chip 2015-01-01 [PMID: 25900329] (IF/IHC, Human)	IF/IHC	Human
Forsberg EA, Botusan I, Wang J et al. Carnosine decreases IGFBP1 production in db/db mice through suppression of HIF-1 J. Endocrinol. 2015-04-13 [PMID: 25869614] (WB, Mouse)	WB	Mouse
Wu S, Zhang J, Huang C et al. Prolyl hydroxylase domain-2 silencing induced by hydrodynamic limb vein injection enhances vascular regeneration in critical limb ischemia mice through activation of multiple genes. Curr Gene Ther 2015-01-01 [PMID: 25832622] (Mouse)		Mouse
Henique C, Mansouri A, Vavrova E et al. Increasing mitochondrial muscle fatty acid oxidation induces skeletal muscle remodeling toward an oxidative phenotype FASEB J. 2015-02-23 [PMID: 25713059] (WB, Mouse)	WB	Mouse
Matak P, Heinis M, Mathieu JR et al. Myeloid HIF-1 Is Protective in Helicobacter pylori-Mediated Gastritis J. Immunol. 2015-02-20 [PMID: 25710915] (IHC-P, Human)	IHC-P	Human
Teranishi Y, Matsubara T, Krausz KW et al. Involvement of hepatic stellate cell cytoglobin in acute hepatocyte damage through the regulation of CYP2E1-mediated xenobiotic metabolism Lab. Invest. 2015-02-16 [PMID: 25686096] (WB, Mouse)	WB	Mouse
Nusken E, Herrmann Y, Wohlfarth M et al. Strong hypoxia reduces leptin synthesis in purified primary human trophoblasts Placenta. 2015-02-03 [PMID: 25684657] (WB, Human)	WB	Human
Robin E, Marcillac F, Raddatz E. A hypoxic episode during cardiogenesis down-regulates the adenosinergic system and alters the myocardial anoxic tolerance. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2015-01-28 [PMID: 25632022] (WB, Chicken)	WB	Chicken
Chang E, Paterno J, Duscher D et al. Exercise induces stromal cell-derived factor-1alpha-mediated release of endothelial progenitor cells with increased vasculogenic function Plast. Reconstr. Surg. 2015-02-01 [PMID: 25626819] (WB, Human)	WB	Human
Palsson-McDermott EM, Curtis AM, Goel G et al. Pyruvate Kinase M2 Regulates Hif-1alpha Activity and IL-1beta Induction and Is a Critical Determinant of the Warburg Effect in LPS-Activated Macrophages Cell Metab. 2015-01-06 [PMID: 25565206] (WB, Mouse) Details: PKM1 antibody used for WB on lysates of LPS-stimulated bone marrow derived macrophages/BMDMs which were isolated from C57BL/6 mice (Figure 1D). WB also performed on BMDMs from mice carrying a PKM2fl/fl allele and relative PKM2+/+ controls were treated or not with 600 nM Tamoxifen for 72 hours, followed by LPS for 24 hours (Figure 5C).	WB	Mouse
Tsuboi I, Yamashita T, Nagano M et al. Impaired expression of HIF -2alpha induces compensatory expression of HIF-1alpha for the recovery from anemia J. Cell. Physiol. 2015-01-03 [PMID: 25557133] (Chemotaxis, WB, Mouse)	Chemotaxis, WB	Mouse
Mai J, Wang F, Qiu Q et al. Tachycardia pacing induces myocardial neovascularization and mobilizes circulating endothelial progenitor cells partly via SDF-1 pathway in canines. Heart Vessels 2016-02-01 [PMID: 25491934]
Kong Ly, Wei J, Haider As et al. Therapeutic targets in subependymoma. J. neuroimmunol. 2014-10-31 [PMID: 25465288] (WB, Human)	WB	Human
Appel S, Turnwald Em, Ankerne J et al. Hypoxia-Mediated Soluble Fms-Like Tyrosine Kinase 1 Increase is not Attenuated in Interleukin 6-Deficient Mice. Reprod Sci. 2014-11-18 [PMID: 25415335]
Kim HR, Kim JH, Choi EJ et al. Hyperoxygenation Attenuated a Murine Model of Atopic Dermatitis through Raising Skin Level of ROS. PLoS ONE. 2014-10-03 [PMID: 25275529] (IHC-P, Mouse) Details: IL-17/IL-17A antibody used for IHC-P on the AD lesions from hyperbaric oxygen therapy (HBOT) or applying an oxygen-carrying chemical, perfluorodecalin (PFD) treated murine model of AD that was developed by repeated application of a chemical irritant (1% 2,4-dinitrochlorobenzene) and house dust mite (Dermatophagoide farinae) extract on one ear of BALB/c mice. Figure 6 shows a decreased IL-17A expression in the AD lesions of HBOT or PFD treated mice.	IHC-P	Mouse
Kim M, Neinast MD, Frank AP et al. ERalpha upregulates Phd3 to ameliorate HIF-1 induced fibrosis and inflammation in adipose tissue Mol Metab. 2014-09-01 [PMID: 25161887] (WB, IP, Mouse) Details: HIF-1 alpha antibody used for IP and WB applications in HIF-1 alpha ubiquitination assay on NIH3T3 cells that were transfected with expression vectors for pcDNA-mHIF1a, myc-Ub1, pFlag-ERa and shPHD3 (Figure 2D).	WB, IP	Mouse
Roychowdhury S, Chiang DJ, McMullen MR et al. Moderate, chronic ethanol feeding exacerbates carbon-tetrachloride-induced hepatic fibrosis via hepatocyte-specific hypoxia inducible factor 1a. Pharmacol Res Perspect 2014-10-01 [PMID: 25089199] (IHC-Fr, Mouse) Details: HIF1a/HIF-1 alpha antibody used for IHC-Fr on frozen liver sections of pair- or ethanol-fed C57BL/6J wild-type mice (Figure 1B) and hepatocyte-specific HIF1a-deficint mice /delta HepHIF1a-/- on C57BL/6 background fed ethanol or not + CCl4 injections 2X/week for 5 weeks (Figure S1).	IHC-Fr	Mouse
Appel S, Ankerne J, Appel J et al. CNN3 Regulates Trophoblast Invasion and Is Upregulated by Hypoxia in BeWo Cells. PLoS ONE 2014-07-23 [PMID: 25050546] (WB, Human) Details: HIF-1 alpha antibody used for WB at 1:1000 dilution as hypoxia marker in BeWo cells grown under normoxic or hypoxic conditions for 24 (Figure 5A) as well as serum starved BeWo cells treated with PBS or 200 uM CoCl2 for 6 h (Figure 5B).	WB	Human
Colegio OR, Chu NQ, Szabo AL et al. Functional polarization of tumour-associated macrophages by tumour-derived lactic acid. Nature 2014-07-13 [PMID: 25043024] (WB, Mouse) Details: HIF1 alpha antibody used for WB on mouse's macrophage cells grown under normoxia /hypoxia and on cells stimulated with control DMEM or Lewis lung carcinoma tumor conditioned medium (Extended Data Figure 2a).	WB	Mouse
Ciavardelli D, Rossi C, Barcaroli D et al. Breast cancer stem cells rely on fermentative glycolysis and are sensitive to 2-deoxyglucose treatment. Cell Death Dis 2014-07-18 [PMID: 25032859] (WB, Human) Details: HIF1 alpha antibody used for WB on human breast cancer tissues derived breast cancer stem cells (BCSCs) and SDAC, spheroid-derived adherent cells (Figure 2d).	WB	Human
Rawluszko-Wieczorek A A, Horbacka K et al. Prognostic potential of DNA methylation and transcript levels of HIF1A and EPAS1 in colorectal cancer. Mol Cancer Res 2014-01-08 [PMID: 24825851] (WB, Human)	WB	Human
O?reilly VC, Floro KL, Shi H et al. Gene-environment interaction demonstrates the vulnerability of the embryonic heart. Dev. Biol. 2014-03-25 [PMID: 24657234] (WB, IHC-P, IHC-Fr, Mouse)	WB, IHC-P, IHC-Fr	Mouse
Duan LJ, Takeda K, Fong GH. Hematological, Hepatic, and Retinal Phenotypes in Mice Deficient for Prolyl Hydroxylase Domain (PHD) Proteins in the Liver. Am. J. Pathol. 2014-02-19 [PMID: 24508125] (WB, Mouse)	WB	Mouse
Duan LJ, Takeda K, Fong GH. Hypoxia inducible factor-2 alpha regulates the development of retinal astrocytic network by maintaining adequate supply of astrocyte progenitors. PLoS ONE 2014-01-29 [PMID: 24475033] (WB, Mouse)	WB	Mouse
Reischl S, Li L, Walkinshaw G et al. Inhibition of HIF prolyl-4-hydroxylases by FG-4497 reduces brain tissue injury and edema formation during ischemic stroke. PLoS ONE 2014-01-10 [PMID: 24409307] (WB, Mouse)	WB	Mouse
Rosafio K, Pellerin L. Oxygen tension controls the expression of the monocarboxylate transporter MCT4 in cultured mouse cortical astrocytes via a hypoxia-inducible factor-1alpha-mediated transcriptional regulation. Glia 2013-12-21 [PMID: 24375723] (WB, Mouse)	WB	Mouse
Ouyang X, Ghani A, Malik A et al. Adenosine is required for sustained inflammasome activation via the A2A receptor and the HIF-1alpha pathway. Nat Commun 2013-12-19 [PMID: 24352507] (WB, Mouse)	WB	Mouse
Zhu Y, Zhang L, Gidday JM. Role of hypoxia-inducible factor-1alpha in preconditioning-induced protection of retinal ganglion cells in glaucoma. Mol Vis. 2013-11-23 [PMID: 24319330] (ICC/IF, IHC-P, Mouse)	ICC/IF, IHC-P	Mouse
Weidemann A, Breyer J, Rehm M et al. HIF-1alpha activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells. Cell Commun Signal. 2013-10-21 [PMID: 24144209] (WB, Mouse)	WB	Mouse
Doedens AL, Phan AT, Stradner MH et al. Hypoxia-inducible factors enhance the effector responses of CD8+ T cells to persistent antigen. Nat Immunol. 2013-11-01 [PMID: 24076634] (WB, Mouse)	WB	Mouse
Zhao H, Luo X, Zhou Z et al. Early treatment with xenon protects against the cold ischemia associated with chronic allograft nephropathy in rats. Kidney Int. 2013-09-11 [PMID: 24025645] (IHC-P, Rat)	IHC-P	Rat
Brackmann FA, Link AS, Jung S et al. Activin A regulation under global hypoxia in developing mouse brain. Brain Res 2013-07-31 [PMID: 23916668] (WB, Mouse)	WB	Mouse
Clinkenbeard EL, Farrow EG, Summers LJ et al. Neonatal iron deficiency causes abnormal phosphate metabolism by elevating FGF23 in normal and ADHR mice. J Bone Miner Res 2013-07-22 [PMID: 23873717]
Sugimoto M, Cutler A, Shen B et al. Inhibition of EGF Signaling Protects the Diabetic Retina from Insulin-Induced Vascular Leakage. Am J Pathol 2013-07-03 [PMID: 23831329] (WB, Human)	WB	Human
Piao Y, Liang J, Holmes L et al. Acquired Resistance to Anti-VEGF Therapy in Glioblastoma Is Associated with a Mesenchymal Transition. Clin Cancer Res 2013-08-15 [PMID: 23804423] (IHC-P, Human)	IHC-P	Human
Miranda E, Nordgren IK, Male AL et al. A cyclic peptide inhibitor of HIF-1 heterodimerization that inhibits hypoxia signaling in cancer cells. J Am Chem Soc. 2013-07-17 [PMID: 23796364] (Human)		Human
Oh JH, Oh J, Togloom A et al. Effects of Ginkgo biloba Extract on Cultured Human Retinal Pigment Epithelial Cells under Chemical Hypoxia. Curr Eye Res 2013-06-21 [PMID: 23790153] (ICC/IF, Human)	ICC/IF	Human
Danza G, Di Serio C, Ambrosio MR et al. Notch3 is activated by chronic hypoxia and contributes to the progression of human prostate cancer. Int J Cancer 2013-06-01 [PMID: 23729168] (WB, Human)	WB	Human
Pawlak EA, Geor RJ, Watts MR et al. Regulation of hypoxia inducible factor-1alpha and related genes in equine digital lamellae and in cultured keratinocytes. Equine Vet J 2013-04-18 [PMID: 23663159]
Balamurugan Kuppusamy, Sharan Shikha, Klarmann Kimberly D et al. FBXW7alpha attenuates inflammatory signalling by downregulating C/EBPdelta and its target gene Tlr4. Nat Commun. 2013-01-01 [PMID: 23575666] (WB, Mouse, Human)	WB	Mouse, Human
Tannahill GM, Curtis AM, Adamik J et al. Succinate is an inflammatory signal that induces IL-1beta through HIF-1alpha Nature 2013-03-24 [PMID: 23535595] (WB, Mouse)	WB	Mouse
Baek Kwang Je, Cho Jae Youn, Rosenthal Peter et al. Hypoxia potentiates allergen induction of HIF-1a, chemokines, airway inflammation, TGF-B1, and airway remodeling in a mouse model. Clinical Immunology (Orlando, Fla.) 2013-01-01 [PMID: 23499929] (IF/IHC, Mouse)	IF/IHC	Mouse
Kocemba KA, van Andel H, de Haan-Kramer A et al. The hypoxia target adrenomedullin is aberrantly expressed in multiple myeloma and promotes angiogenesis Leukemia 2013-03-12 [PMID: 23478664] (WB, Human)	WB	Human
Longati P, Jia X, Eimer J et al. 3D pancreatic carcinoma spheroids induce a matrix-rich, chemoresistant phenotype offering a better model for drug testing BMC Cancer 2013-02-27 [PMID: 23446043] (WB, Human)	WB	Human
Huang C, Hales BF. Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs PLoS One 2012-01-01 [PMID: 23251655] (IF/IHC, Mouse)	IF/IHC	Mouse
Sun K, Halberg N, Khan M et al. Selective Inhibition of HIF1alpha Ameliorates Adipose Tissue Dysfunction Mol Cell Biol 2012-12-17 [PMID: 23249949] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Deschepper M, Manassero M, Oudina K et al. Proangiogenic and Prosurvival Functions of Glucose in Human Mesenchymal Stem Cells upon Transplantation. Stem Cells 2013-03-01 [PMID: 23225732] (WB, Human)	WB	Human
Riddell JR, Maier P, Sass SN et al. Peroxiredoxin 1 Stimulates Endothelial Cell Expression of VEGF via TLR4 Dependent Activation of HIF-1alpha PLoS One 2012-01-01 [PMID: 23185615] (IF/IHC, WB, Mouse, Human)	IF/IHC, WB	Mouse, Human
Neumeister VM, Anagnostou V, Siddiqui S et al. Quantitative Assessment of Effect of Preanalytic Cold Ischemic Time on Protein Expression in Breast Cancer Tissues J Natl Cancer Inst 2012-10-22 [PMID: 23090068] (IF/IHC, Human)	IF/IHC	Human
Xiao H, Li S, Zhang D et al. Separate and concurrent use of 2-deoxy-D-glucose and 3-bromopyruvate in pancreatic cancer cells. Oncol Rep. 2013-01-01 [PMID: 23076497] (WB, Human)	WB	Human
Shin MK, Drager LF, Yao Q et al. Metabolic Consequences of High-Fat Diet Are Attenuated by Suppression of HIF-1alpha PLoS One 2012-01-01 [PMID: 23049707] (WB, Mouse)	WB	Mouse
Veith C, Marsh LM, Wygrecka M et al. Paxillin regulates pulmonary arterial smooth muscle cell function in pulmonary hypertension. Am J Pathol 2012-11-01 [PMID: 22959909]
Kunze R, Zhou W, Veltkamp R et al. Neuron-specific prolyl-4-hydroxylase domain 2 knockout reduces brain injury after transient cerebral ischemia. Stroke 2012-10-01 [PMID: 22933585] (Mouse)		Mouse
Kottmann RM, Kulkarni AA, Smolnycki KA et al. Lactic Acid Is Elevated in Idiopathic Pulmonary Fibrosis and Induces Myofibroblast Differentiation via pH-Dependent Activation of Transforming Growth Factor-beta. Am J Respir Crit Care Med 2012-10-15 [PMID: 22923663]
Hamidi T, Cano CE, Grasso D et al. Nupr1-aurora kinase A pathway provides protection against metabolic stress-mediated autophagic-associated cell death. Clin Cancer Res 2012-10-01 [PMID: 22899799]
Ji K, Tsirka SE. Inflammation modulates expression of laminin in the central nervous system following ischemic injury. J Neuroinflammation 2012-07-01 [PMID: 22759265]
Pohjoismaki JL, Boettger T, Liu Z, Goffart S, Szibor M, Braun T. Oxidative stress during mitochondrial biogenesis compromises mtDNA integrity in growing hearts and induces a global DNA repair response. Nucleic Acids Res. 2012-04-15 [PMID: 22508755] (WB, Rat)	WB	Rat
Wu MH, Lu CW, Chang FM, Tsai SJ. Estrogen receptor expression affected by hypoxia inducible factor-1 alpha in stromal cells from patients with endometriosis Taiwan J Obstet Gynecol 2012-03-01 [PMID: 22482968] (WB, Human)	WB	Human
Rawat S Maturation of Cardiac Energy Metabolism in the Normal and Hypertrophied Newborn Heart Am J Physiol Heart Circ Physiol 2012-03-13 [PMID: 22408020]
Gayed BA, O'Malley KJ, Pilch J, Wang Z. Digoxin Inhibits Blood Vessel Density and HIF-1a Expression in Castration-Resistant C4-2 Xenograft Prostate Tumors. Clin Transl Sci;;5(1):39-42. doi: 10.1111/j.1752-8062.2011.00376.x. 2012-02-01 [PMID: 22376255]
Shiau AL, Shen YT, Hsieh JL et al. Scutellaria barbata inhibits angiogenesis through downregulation of HIF-1 alpha in lung tumor. Environ Toxicol 2014-04-01 [PMID: 22331677]
Chiusa M, Hool SL, Truetsch P, Djafarzadeh S, Jakob SM, Seifriz F, Scherer SJ, Suter TM, Zuppinger C, Zbinden S. Cancer therapy modulates VEGF signaling and viability in adult rat cardiac microvascular endothelial cells and cardiomyocytes. J Mol Cell Cardiol. 2012-02-03 [PMID: 22326847]
Zhang L, Das P, Schmolke M et al. Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export. J Cell Biol 2012-02-01 [PMID: 22312003]
Copple BL, Kaska S, Wentling C. Hypoxia-inducible factor activation in myeloid cells contributes to the development of liver fibrosis in cholestatic mice. J Pharmacol Exp Ther. 2012-05-01 [PMID: 22271822] Details: IHC (frozen): Fig 1B, D (mouse liver). The Cre antibody (IMG-5720. IMG-5721 or IMG-5722) was used at 1:4000.
Barta P, Van Pelt C, Men T et al. Enhancement of lung tumorigenesis in a Gprc5a Knockout mouse by chronic extrinsic airway inflammation Mol Cancer 2012-01-12 [PMID: 22239913] (IF/IHC, Mouse)	IF/IHC	Mouse
KIYOSHI OKAMOTO, DAISUKE ITO, KAZUKI MIYAZAKI, SAORI WATANABE, OSAMU TOHYAMA, AKIRA YOKOI, YOICHI OZAWA, MAKOTO ASANO, TAKANORI KAWAMURA, YOSHINOBU YAMANE, SATOSHI NAGAO, SETSUO FUNASAKA, JUNICHI KAMATA, YOSHIHIKO KOTAKE, MIKA AOKI, NAOKO TSUKAHARA, YOSHIHARU MIZUI, ISAO TANAKA, KOHEI SAWADA Microregional antitumor activity of a small-molecule hypoxia-inducible factor 1 inhibitor International Journal of Molecular Medicine 2011-12-29 [PMID: 22211243]
Ko SH, Nauta A, Morrison SD et al. Antimycotic ciclopirox olamine in the diabetic environment promotes angiogenesis and enhances wound healing. PLoS ONE 20116(11). [PMID: 22125629]
Hong SJ, Jin da P, Buck DW 2nd et al. Impaired response of mature adipocytes of diabetic mice to hypoxia. Exp Cell Res 2011-10-01 [PMID: 21777581]
Rabie T, Kunze R, Marti HH. Impaired hypoxic response in senescent mouse brain. Int J Dev Neurosci. 2011-06-16 [PMID: 21704147]
Jantsch J, Wiese M, Schodel J et al. Toll-like receptor activation and hypoxia use distinct signaling pathways to stabilize hypoxia-inducible factor 1{alpha} (HIF1A) and result in differential HIF1A-dependent gene expression. J Leukoc Biol. 2011-06-17 [PMID: 21685248]
Pascal LE, Ai J, Rigatti LH et al. EAF2 loss enhances angiogenic effects of Von Hippel-Lindau heterozygosity on the murine liver and prostate. Angiogenesis 2011-01-01 [PMID: 21638067] (WB, Mouse)	WB	Mouse
Guadall A, Orriols M, Alcudia JF et al. Hypoxia-induced ROS signaling is required for LOX up-regulation in endothelial cells Front Biosci 2011-06-01 [PMID: 21622104] (WB, Human)	WB	Human
Jiang X, Khan MA, Tian W et al. Adenovirus-mediated HIF-1alpha gene transfer promotes repair of mouse airway allograft microvasculature and attenuates chronic rejection Clin Invest 2011-06-01 [PMID: 21606594]
Fedorovich N E, Kuipers E et al. Scaffold porosity and oxygenation of printed hydrogel constructs affect functionality of embedded osteogenic progenitors. Tissue Eng Part A 2011-01-10 [PMID: 21599540] (IHC-P, Chicken, Goat)	IHC-P	Chicken, Goat
Duan LJ, Takeda K, Fong GH et al. Prolyl hydroxylase domain protein 2 (PHD2) mediates oxygen-induced retinopathy in neonatal mice Am J Pathol. 2011-04-01 [PMID: 21435465] (WB, Mouse)	WB	Mouse
Dunford ECE, Herbst EAF, Jeoung NH et al. Pyruvate dehydrogenase (PDH) activation during in vitro muscle contractions in PDH kinase 2 knockout mice: the effect of PDH kinase 1 compensation. Am J Physiol Regul Integr Comp Physiol. 2011-03-16 [PMID: 21411764]
Wolff NC, Vega-Rubin-de-Celis S, Xie XJ et al. Cell-type-dependent regulation of mTORC1 by REDD1 and the tumor suppressors TSC1/TSC2 and LKB1 in response to hypoxia. Mol Cell Biol 2011-05-01 [PMID: 21383064]
Nagao K, Oka K. HIF-2 directly activates CD82 gene expression in endothelial cells. Biochem Biophys Res Commun;407(1):260-5. 2011-04-01 [PMID: 21382346]
Camacho M, Rodriguez C, Guadall A et al. Hypoxia upregulates PGI-synthase and increases PGI2 release in human vascular cells exposed to inflammatory stimuli J Lipid Res 2011-04-01 [PMID: 21296955] (WB, Human)	WB	Human
Li Q, Michaud M, Canosa S, Kuo A, Madri JA. GSK-3?: a signaling pathway node modulating neural stem cell and endothelial cell interactions. Angiogenesis;14(2):173-85. 2011-05-01 [PMID: 21253820] (WB, Mouse)	WB	Mouse
Huang M, Wu JC. Molecular imaging of RNA interference therapy targeting PHD2 for treatment of myocardial ischemia. Methods Mol Biol;709:211-21. 2011-01-01 [PMID: 21194030] (WB, Mouse)	WB	Mouse
Doran DM, Kulkarni-Datar K, Cool DR et al. Hypoxia Activates Constitutive Luciferase Reporter Constructs. Biochimie. 2010-10-21 [PMID: 20971156]
Pirkmajer S, Filipovic D, Mars T et al. HIF-1alpha response to hypoxia is functionally separated from the glucocorticoid stress response in the in vitro regenerating human skeletal muscle. Am J Physiol Regul Integr Comp Physiol 2010-12-01 [PMID: 20943857] (WB, Human)	WB	Human
Xu D, Yao Y, Lu L et al. Plk3 functions as an essential component of the hypoxia regulatory pathway by direct phosphorylation of HIF-1alpha. J Biol Chem 2010-12-01 [PMID: 20889502]
Copple BL, Bai S, Burgoon LD, Moon J-O. Hypoxia-inducible factor-1[alpha] regulates the expression of genes in hypoxic hepatic stellate cells important for collagen deposition and angiogenesis. Liver Int. [PMID: 20880076]
Welford SM, Dorie MJ, Li X et al. Renal oxygenation suppresses VHL loss-induced senescence that is caused by increased sensitivity to oxidative stress. Mol Cell Biol 2010-10-01 [PMID: 20679489]
Gimm T, Wiese M, Teschemacher B et al. Hypoxia-inducible protein 2 is a novel lipid droplet protein and a specific target gene of hypoxia-inducible factor-1 FASEB J 2010-11-01 [PMID: 20624928] (WB, Human)	WB	Human
Tam KK, Russell DL, Peet DJ et al. Hormonally regulated follicle differentiation and luteinization in the mouse is associated with hypoxia inducible factor activity. Mol Cell Endocrinol 2010-10-07 [PMID: 20600586] (WB, Mouse)	WB	Mouse
Mikheyev AS, Vo T, Wee B et al. Rapid microsatellite isolation from a butterfly by de novo transcriptome sequencing: performance and a comparison with AFLP-derived distances. PLoS One 2010-01-01 [PMID: 20585453]
Copple BL, Bai S, Moon JO. Hypoxia-inducible factor-dependent production of profibrotic mediators by hypoxic Kupffer cells. Hepatol Res;40(5):530-9. 2010-05-01 [PMID: 20412331]
Lu ZH, Shvartsman MB, Lee AY et al. Mammalian target of rapamycin activator RHEB is frequently overexpressed in human carcinomas and is critical and sufficient for skin epithelial carcinogenesis. Cancer Res. 2010-04-15 [PMID: 20388784] Details: Breast Cancer Exploratory Array (IMA-100). IHC (paraffin): Normal human breast tissues, ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC), stained with RHEB antibody, Fig 1A, 1B, & 1C.Note: Examples RHEB staining on normal lobule, DCIS, and IDC, is shown on Fig 1A.
Serra-Perez A, Planas AM, Nunez-O'Mara A et al. Extended ischemia prevents HIF1alpha degradation at reoxygenation by impairing prolyl-hydroxylation: role of Krebs cycle metabolites. J Biol Chem 2010-06-11 [PMID: 20368331]
Costa B, Dettori D, Lorenzato A et al. Fumarase tumor suppressor gene and MET oncogene cooperate in upholding transformation and tumorigenesis FASEB J. 2010-08-01 [PMID: 20354140]
Kuphal S, Winklmeier A, Warnecke C, Bosserhoff AK. Constitutive HIF-1 activity in malignant melanoma Eur J Cancer 2010-04-01 [PMID: 20185296] (WB, Human)	WB	Human
Clever JL, Sakai Y, Wang RA et al. Inefficient skeletal muscle repair in inhibitor of differentiation knockout mice suggests a crucial role for BMP signaling during adult muscle regeneration. Am J Physiol Cell Physiol;298(5):C1087-99. 2010-05-01 [PMID: 20181926] (WB, Mouse)	WB	Mouse
Copple BL. Hypoxia stimulates hepatocyte epithelial to mesenchymal transition by hypoxia-inducible factor and transforming growth factor-beta-dependent mechanisms Liver Int 2010-05-01 [PMID: 20158611] (WB, Mouse)	WB	Mouse
el Filali M, Missotten GS, Maat W et al. Regulation of VEGF-A in uveal melanoma. Invest Ophthalmol Vis Sci 2010-05-01 [PMID: 20042655]
Porcelli AM, Ghelli A, Ceccarelli C et al. The genetic and metabolic signature of oncocytic transformation implicates HIF1alpha destabilization. Hum Mol Genet 2010-03-01 [PMID: 20028790]
Samarin J, Wessel J, Cicha I et al. FoxO proteins mediate hypoxic induction of connective tissue growth factor in endothelial cells. J Biol Chem 2010-02-12 [PMID: 20018872]
S K Martin, P Diamond, S A Williams et al. Hypoxia-inducible factor-2 is a novel regulator of aberrant CXCL12 expression in multiple myeloma plasma cells. Haematologica; 95 (5): 776-784. 2010-01-01 [PMID: 20015878]
Volke M, Gale DP, Maegdefrau U et al. Evidence for a lack of a direct transcriptional suppression of the iron regulatory peptide hepcidin by hypoxia-inducible factors. PLoS One 2009-11-18 [PMID: 19924283] (WB, Human)	WB	Human
Tanaka T, Wiesener M, Bernhardt W et al. The human HIF (hypoxia-inducible factor)-3alpha gene is a HIF-1 target gene and may modulate hypoxic gene induction. Biochem J 2009-11-15 [PMID: 19694616]
Loinard C, Ginouves A, Vilar J et al. Inhibition of prolyl hydroxylase domain proteins promotes therapeutic revascularization. Circulation 2009-07-01 [PMID: 19546390]
Gao Peng, Zhu Yiqian, Ling Feng et al. Nonischemic cerebral venous hypertension promotes a pro-angiogenic stage through HIF-1 downstream genes and leukocyte-derived MMP-9. J Cereb Blood Flow Metab 2009-01-01 [PMID: 19471278] (IF/IHC, Mouse)	IF/IHC	Mouse
Huang C, Hales BF. Teratogen responsive signaling pathways in organogenesis stage mouse limbs. Reprod Toxicol 2009-04-01 [PMID: 19429390] (WB, Mouse, Primate)	WB	Mouse, Primate
Copple BL, Bustamante JJ, Welch TP et al. Hypoxia-inducible factor-dependent production of profibrotic mediators by hypoxic hepatocytes. Liver Int 2009-08-01 [PMID: 19302442] (ICC/IF, WB, Mouse)	ICC/IF, WB	Mouse
Xiao W, Ai J, Habermacher G et al. U19/Eaf2 Binds to Stabilizes von Hippel-Lindau Protein. Cancer Res;69(6):2599-2606. 2009-01-01 [PMID: 19258512] (WB)	WB
Houten SM, Chegary M, Te Brinke H et al. Pyruvate dehydrogenase kinase 4 expression is synergistically induced by AMP-activated protein kinase and fatty acids. Cell Mol Life Sci 2009-04-01 [PMID: 19224132] (WB, Rat)	WB	Rat
Lomb DJ, Desouza LA, Franklin JL et al. Prolyl Hydroxylase Inhibitors Depend on Extracellular Glucose Hypoxia-Inducible Factor (HIF)-2{alpha} to Inhibit Cell Death Caused by Nerve Growth Factor (NGF) Deprivation: Evidence that HIF-2{alpha} Has a Role in NGF-Promoted Survival of Sympathetic Neurons. Mol Pharmacol;75(5):1198-1209. 2009-01-01 [PMID: 19204094] (WB)	WB
Vasseur S, Afzal S, Tardivel-Lacombe J et al. DJ-1/PARK7 is an important mediator of hypoxia-induced cellular responses. Proc Natl Acad Sci U S A 2009-01-01 [PMID: 19144925]
Moon J-OK, Welch TP, Gonzalez FJ et al. Reduced liver fibrosis in hypoxia-inducible factor-1{alpha}-deficient mice. Am J Physiol Gastrointest Liver Physiol;296(3):G582-592. 2009-01-01 [PMID: 19136383] (ICC/IF, IHC-Fr, WB, Mouse)	ICC/IF, IHC-Fr, WB	Mouse
Lu C-W, Lin S-C, Chen K-F et al. Induction of Pyruvate Dehydrogenase Kinase-3 by Hypoxia-inducible Factor-1 Promotes Metabolic Switch Drug Resistance. J Biol Chem;283(42):28106-28114. 2008-01-01 [PMID: 18718909] (WB, Human)	WB	Human
Shui YB, Arbeit JM, Johnson RS et al. HIF-1: an age-dependent regulator of lens cell proliferation. Invest Ophthalmol Vis Sci 2008-11-01 [PMID: 18586877]
Yang Y, Bai J, Shen R et al. Polo-like kinase 3 functions as a tumor suppressor and is a negative regulator of hypoxia-inducible factor-1 alpha under hypoxic conditions. Cancer Res 2008-06-01 [PMID: 18519666]
Wu S, Nishiyama N, Kano MR et al. Enhancement of angiogenesis through stabilization of hypoxia-inducible factor-1 by silencing prolyl hydroxylase domain-2 gene. Mol Ther 2008-07-01 [PMID: 18500250]
Kanasaki K, Palmsten K, Sugimoto H et al. Deficiency in catechol-O-methyltransferase and 2-methoxyoestradiol is associated with pre-eclampsia. Nature 2008-06-19 [PMID: 18469803]
Warnecke C, Weidemann A, Volke M et al. The specific contribution of hypoxia-inducible factor-2alpha to hypoxic gene expression in vitro is limited and modulated by cell type-specific and exogenous factors. Exp Cell Res 2008-06-10 [PMID: 18420194] (Human)		Human
Vangeison G, Carr D, Federoff HJ et al. The good, the bad, and the cell type-specific roles of hypoxia inducible factor-1 alpha in neurons and astrocytes. J Neurosci;28(8):1988-93. 2008-02-20 [PMID: 18287515] (WB, Mouse)	WB	Mouse
Sun L, Marti HH, Veltkamp R. Hyperbaric oxygen reduces tissue hypoxia and hypoxia-inducible factor-1 alpha expression in focal cerebral ischemia. Stroke;39(3):1000-6. 2008-03-01 [PMID: 18239183] (IHC-P, ICC/IF, Mouse)	IHC-P, ICC/IF	Mouse
Becker CM, Rohwer N, Funakoshi T et al. 2-methoxyestradiol inhibits hypoxia-inducible factor-1{alpha} and suppresses growth of lesions in a mouse model of endometriosis. Am J Pathol;172(2):534-44. 2008-02-01 [PMID: 18202195] (WB, IHC-P, Mouse)	WB, IHC-P	Mouse
Scortegagna M, Cataisson C, Martin RJ et al. HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling. Blood 2008-04-01 [PMID: 18199827]
May D, Gilon D, Djonov V et al. Transgenic system for conditional induction and rescue of chronic myocardial hibernation provides insights into genomic programs of hibernation. Proc Natl Acad Sci U S A;105(1):282-7. 2008-01-08 [PMID: 18162550] (IHC-P, Mouse)	IHC-P	Mouse
Takeda K, Aguila HL, Parikh NS et al. Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins. Blood;111(6):3229-35. 2008-03-15 [PMID: 18056838] (WB, Mouse)	WB	Mouse
Friese MA, Craner MJ, Etzensperger R et al. Acid-sensing ion channel-1 contributes to axonal degeneration in autoimmune inflammation of the central nervous system. Nat Med 2007-12-01 [PMID: 17994101] (Mouse)		Mouse
Glassford AJ, Yue P, Sheikh AY et al. HIF-1 regulates hypoxia- and insulin-induced expression of apelin in adipocytes. Am J Physiol Endocrinol Metab;293(6):E1590-6. 2007-12-01 [PMID: 17878221] (WB, Mouse)	WB	Mouse
Palmer LA, Doctor A, Chhabra P et al. S-nitrosothiols signal hypoxia-mimetic vascular pathology. J Clin Invest;117(9):2592-601. 2007-09-01 [PMID: 17786245] (WB, Mouse)	WB	Mouse
Takeda K, Cowan A, Fong GH et al. Essential role for prolyl hydroxylase domain protein 2 in oxygen homeostasis of the adult vascular system Circulation. 2007-08-14 [PMID: 17646578] (WB, Mouse)	WB	Mouse
Chen HL, Pistollato F, Hoeppner DJ et al. Oxygen tension regulates survival and fate of mouse central nervous system precursors at multiple levels. Stem Cells;25(9):2291-301. 2007-09-01 [PMID: 17556599] (ELISA, Mouse)	ELISA	Mouse
van der Bilt JD, Soeters ME, Duyverman AM et al. Perinecrotic hypoxia contributes to ischemia/reperfusion-accelerated outgrowth of colorectal micrometastases. Am J Pathol 2007-04-01 [PMID: 17392176] (Mouse)		Mouse
Nagano M, Yamashita T, Hamada H et al. Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood;110(1):151-60. 2007-07-01 [PMID: 17379743] (WB, Human)	WB	Human
Shum LC. Mitochondrial Metabolism in Bone Physiology and Pathology Thesis 2018-01-01 (WB, Human)	WB	Human
van de Sluis B, Muller P, Duran K et al. Increased activity of hypoxia-inducible factor 1 is associated with early embryonic lethality in Commd1 null mice. Mol Cell Biol 2007-06-01 [PMID: 17371845]
Bedogni B, Welford SM, Kwan AC et al. Inhibition of phosphatidylinositol-3-kinase and mitogen-activated protein kinase kinase 1/2 prevents melanoma development and promotes melanoma regression in the transgenic TPRas mouse model. Mol Cancer Ther 2006-12-01 [PMID: 17172409]
Welford SM, Bedogni B, Gradin K et al. HIF1alpha delays premature senescence through the activation of MIF. Genes Dev 2006-12-01 [PMID: 17142669]
Kim WY, Safran M, Buckley MR et al. Failure to prolyl hydroxylate hypoxia-inducible factor alpha phenocopies VHL inactivation in vivo. EMBO J 2006-10-04 [PMID: 16977322]
Takeda K, Ho VC, Takeda H et al. Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2. Mol Cell Biol;26(22):8336-46. 2006-11-01 [PMID: 16966370] (ELISA, WB, Mouse)	ELISA, WB	Mouse
Litz J, Krystal GW. Imatinib inhibits c-Kit-induced hypoxia-inducible factor-1alpha activity and vascular endothelial growth factor expression in small cell lung cancer cells. Mol Cancer Ther 2006-06-01 [PMID: 16818499] (Human)		Human
Martin-Oliva D, Aguilar-Quesada R, O'valle F et al. Inhibition of poly(ADP-ribose) polymerase modulates tumor-related gene expression, including hypoxia-inducible factor-1 activation, during skin carcinogenesis. Cancer Res 2006-06-01 [PMID: 16740713]
Hu CJ, Iyer S, Sataur A et al. Differential regulation of the transcriptional activities of hypoxia-inducible factor 1 alpha (HIF-1alpha) and HIF-2alpha in stem cells Mol Cell Biol 2006-05-01 [PMID: 16611993] (WB)	WB
Zampino M, Yuzhakova M, Hansen J et al. Sex-related dimorphic response of HIF-1 alpha expression in myocardial ischemia. Am J Physiol Heart Circ Physiol 2006-08-01 [PMID: 16603692]
Hsu T, Adereth Y, Kose N et al. Endocytic function of von Hippel-Lindau tumor suppressor protein regulates surface localization of fibroblast growth factor receptor 1 and cell motility. J Biol Chem;281(17):12069-80. 2006-04-28 [PMID: 16505488] (WB, Human)	WB	Human
Frank D B, Abtahi A et al. Bone morphogenetic protein 4 promotes pulmonary vascular remodeling in hypoxic pulmonary hypertension. Circ Res 2005-02-09 [PMID: 16100039] (WB, Mouse)	WB	Mouse
Litz J, Krystal GW. Imatinib inhibits c-Kit-induced hypoxia-inducible factor-1alpha activity and vascular endothelial growth factor expression in small cell lung cancer cells. Mol Cancer Ther;5(6):1415-22. 2006-06-01 [PMID: 1681849] (WB, Human)	WB	Human
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Reviews for HIF-1 alpha Antibody (NB100-449) (29) 4.329

Average Rating: 4.3

(Based on 29 reviews)

We have 29 reviews tested in 5 species: Human, Mouse, Rat, Goat, Other.

Reviews using NB100-449:

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Ratings

Applications

Species

Date

Details

reviewed by:
Verified Customer

Mouse

01/11/2024

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Summary

Application	Western Blot
Sample Tested	Hep3B human hepatocellular carcinoma cell line,mouse liver
Species	Mouse
Lot	A07
Pub Med ID	38652538

Comments

Nuclear extraction: NE-PER NUCLEAR AND CYTOPLASMIC
EXTRACTION REAGENTS

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Verified Customer

Mouse

05/08/2020

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Summary

Application	Western Blot
Sample Tested	3T3-L1 mouse embryonic fibroblast adipose-like cell line
Species	Mouse
Lot	lot A3

Comments

skimmed milk 5%
primary : 1:2000
secondary: 1: 200000

Immunohistochemistry-Paraffin HIF-1 alpha NB100-449

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IHC-P

Mouse

06/07/2019

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Summary

Application	Immunohistochemistry-Paraffin
Sample Tested	PDX mouse
Species	Mouse
Lot	Lot A3

Comments

Working Dilution 1:100, EDTA pH 9.0; detection kit Polymer-HRP.

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reviewed by:
an shiyu

Goat

05/15/2018

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Summary

Application	Western Blot
Sample Tested	laydig cell
Species	Goat
Lot	NBP1-04676SS

Comments

dilution:1:500

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reviewed by:
Verified Customer

Mouse

07/17/2017

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Summary

Application	Western Blot
Sample Tested	BMDM
Species	Mouse
Lot	A5

reviewed by:
Verified Customer

Rat

04/24/2017

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Summary

Application	Immunoprecipitation
Sample Tested	PC-12 rat adrenal pheochromocytoma cell line
Species	Rat
Lot	A6

Immunofluorescence HIF-1 alpha NB100-449

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reviewed by:
Verified Customer

Mouse

01/12/2016

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Summary

Application	Immunofluorescence
Sample Tested	primary bone marrow derived macrophages
Species	Mouse

reviewed by:
Verified Customer

Human

09/07/2015

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Summary

Application	Western Blot
Sample Tested	Human cancer cell whole cell lysate
Species	Human

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reviewed by:
Zachary Patinkin

Simple Western

Human

06/09/2015

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Summary

Application	Simple Western
Sample Tested	Human mesenchymal stem cell whole cell lysate
Species	Human
Lot	A5

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reviewed by:
Enrica Borsi

Human

01/09/2015

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Summary

Application	Western Blot
Sample Tested	Myeloma cells whole cell lysate
Species	Human

reviewed by:
Verified Customer

Human

12/12/2014

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Summary

Application	Western Blot
Sample Tested	See PMID 22923663
Species	Human

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reviewed by:
Ming Lu

Human

08/15/2014

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Summary

Application	Western Blot
Sample Tested	Human pancreatic cancer cell lysates
Species	Human
Lot	A4

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reviewed by:
Xiuquan Luo

Human

07/18/2014

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Summary

Application	Western Blot
Sample Tested	Caki-1 cell lysate
Species	Human

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reviewed by:
Verified Customer

Mouse

07/05/2014

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Summary

Application	Western Blot
Sample Tested	large intestinal lamina propria leukocytes (LPLs).
Species	Mouse

reviewed by:
Paramananda Saikia

Mouse

06/30/2014

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Summary

Application	Western Blot
Sample Tested	Mouse
Species	Mouse
Lot	A4

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reviewed by:
Alshaimaa Adawy

Human

04/29/2014

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Summary

Application	Western Blot
Sample Tested	nuclear fractions from T47-D beast cancer cell line
Species	Human
Lot	A5

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reviewed by:
Verified Customer

Other

03/14/2014

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Summary

Application	Western Blot
Species	Other
Lot	A4

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reviewed by:
Lidan Zhao

Other

08/26/2013

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Summary

Application	Western Blot
Sample Tested	pig satellite cell nuclear protein
Species	Other

reviewed by:
Verified Customer

Human

11/05/2012

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Summary

Application	Western Blot
Sample Tested	human cell lysates
Species	Human

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reviewed by:
Annie Curtis

Mouse

08/24/2012

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Summary

Application	Western Blot
Sample Tested	mouse peritoneal and bone marrow derived macrophages
Species	Mouse
Lot	A4

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reviewed by:
kimberley tam

08/07/2012

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Summary

Application	Western Blot
Lot	A2

reviewed by:
Verified Customer

05/09/2012

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Summary

Application

Western Blot

reviewed by:
Verified Customer

Rat

01/03/2012

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Summary

Application	Western Blot
Sample Tested	PC12 cell whole lysate, Sample Amount: 25ug
Species	Rat
Lot	A4

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reviewed by:
Verified Customer

Human

06/08/2011

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Summary

Application	Western Blot
Sample Tested	HUVEC whole cell lysate, Sample Amount: 20 microg
Species	Human

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reviewed by:
Verified Customer

Mouse

12/22/2010

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Summary

Application	Western Blot
Sample Tested	cerebellar neurons
Species	Mouse

Immunoprecipitation HIF-1 alpha NB100-449

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reviewed by:
William Lin

Human

12/03/2010

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Summary

Application	Immunoprecipitation
Sample Tested	HEK 293
Species	Human
Lot	A3

reviewed by:
Verified Customer

Other

03/11/2010

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Summary

Application	Western Blot
Sample Tested	Colon Cancer Cell lines, Sample Amount: 50ug
Species	Other
Lot	A2

reviewed by:
Verified Customer

Mouse

12/21/2009

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Summary

Application	Western Blot
Sample Tested	human tumor model mouse, Sample Amount: 12ug
Species	Mouse

reviewed by:
Verified Customer

Mouse

04/08/2009

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Summary

Application	Western Blot
Sample Tested	Mouse Neuroblastoma, Sample Amount: 25ug
Species	Mouse
Lot	A2

Product General Protocols

View specific protocols for HIF-1 alpha Antibody (NB100-449):

Immunohistochemistry Protocols (NB100-449)

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

ICC/IF Video Protocol

FAQs for HIF-1 alpha Antibody (NB100-449). (Showing 1 - 10 of 14 FAQs).

Why is there a difference between the theoretical MW for HIF1A and the observed MW for HIF-1 alpha?
- HIF1A, like many other proteins, has post-translational modifications. Depending on the size, amount and nature of the post-translational modifications, it can cause subtle to very large changes in molecular weight.
Which antibody(ies) do you recommend for the detection of HIF-1a by immunohistochemistry in the sections of paraffin-embedded mouse liver samples? I would appreciate if you can give me several choices and rank them in the order of performance. My goal is to distinguish HIF upregulation by prolyl hydroxylase inhibitor in different liver cells.
- All of our antibodies are of high quality and are well tested/validated in species/applications we list on the datasheet. However, we suggest the following four HIF-1 alpha antibodies based upon customer reviews, as well as the number of peer reviewed publications in which these products have been cited by researchers from reputed institutes. (1) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-105) (cited in at least 218 peer reviewed publications) (2) HIF-1 alpha Antibody (cat# NB100-479) (cited in at least 51 peer reviewed publications) (3) HIF-1 alpha Antibody (H1alpha67) (cat# NB100-123 ) (cited in at least 38 peer reviewed publications) (4) HIF-1 alpha Antibody (cat# NB100-449) (cited in at least 31 peer reviewed publications).
I would like to know, does a path exist for detection of HIF 1 in venous blood before and after revascularization of the leg?
- We are not entirely sure if HIF-1 alpha will be present in the leg after revascularization. It may be present, but you may want to search the literature to see if this has been looked at before. If not, then this would certainly be an experiment worth doing.
What is the molecular weight (kDa) of protein HIF 1 alpha in western blot?
- The theoretical molecular weight of HIF 1-alpha is ~93kDa. However, you will likely see a band between 100-120kDa due to phosphorylation.
We got the Hif1a (NB100-105) antibody from you guys. I used the concentration that is mentioned on your website, but I am getting a band of a completely different size (~70kDa) and not the 120 kDa mentioned.
- HIF-1 alpha is a notoriously difficult protein to work with due to its rapid degradation. Therefore, the ~70kDa bands are most likely degradation products. It is very important to lyse the cells in hypoxic conditions. We strongly recommend lysing the cells directly into the Laemmli buffer and doing that quickly, so that the exposure to oxygen is minimized.Please go through our hypoxia related FAQs, you should find them very informative.Also, running a positive control may help confirm the band specificity in your samples. You may prepare them yourself or choose some from our catalog, for example: 1) HeLa Hypoxic / Normoxic Cell Lysate (NBP2-36452)2) HeLa Hypoxic (CoCl2) / Normoxic Cell Lysate (NBP2-36450)
I performed several Western Blots of HIF-1 alpha with different lysis buffers, whole lysates, and cytoplasm/nuclei extractions. I can’t seem to get a good western blot (poor signal, band much lower than expected, etc.). Can someone suggest some technical considerations/tricks I should consider using?
- A major issue that researchers working with HIF-1 alpha is degradation due to exposure to oxygen. In western blot, this results in a weaker band and/or the appearance of multiple low molecular weight bands (40-80 kDa). We recommend preparing the lysates after collection of cells/tissues as quickly as possible (on ice), preferably in a hypoxic chamber. We also recommend including a true hypoxia mimetic control (eg: cells treated with CoCl2, DMOG… etc.). The controls help distinguish your band of interest from potential degradation/dimer bands.For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
I am doing HIF1 westerns in HIF-overexpressing mouse liver and adipose tissue using Novus antirabbit HIF1a antibody with overnight incubation. I am getting strong bands around 90kDa. I am aware that HIF theoretical molecular weight is 93kDa, but in westerns, the HIF band is usually around 120kDa according to my internet research. Can someone let me know if I’m getting the right HIF band or just some non-specific bands? Thanks.
- (1) HIF-1 alpha’s theoretical molecular weight is 93kDa. The post translationally modified/ubiquitinated form of HIF-1 alpha protein (fails to undergo proteasomal degradation) shows up as a band in the 110-130 kDa range on a Western blot.(2) The dimeric protein may appear at a position above 200 kDa on non-reducing gels.(3) Importantly, HIFs are among the most rapidly degradable proteins; therefore, sample preparation is highly important when analyzing HIF1 alpha or HIF2 alpha. When degraded, HIF-1 alpha may show up between 40-80 kDa position on Western blot. Degradation may be avoided by preparing the samples as soon as possible after collection of cells/tissues in hypoxic chamber. Notably, the tissues/cells should be kept on ice during lysate preparation and the lysates should be analyzed as soon as possible.(4) For troubleshooting suggestions/feedback on more than 25 similar frequently asked questions, I would recommend visiting Novus page: FAQs - Hypoxia and HIFs (5) Last but not the least, Novus technical support team may be contacted via email
I have Hif1a nuclear protein extract at -80C. I am wondering if anyone knows how long it would be good for at that temperature since HIf1a is known to be degraded easily.Thank you!
- You could try a few things to further inhibit the degradation.1) Use the protease inhibitors (if you are not already using them).2) Lyse cells into a buffer that contains SDS or LDS (eg: Laemmli's buffer), since SDS and LDS denature and inhibit proteases. Lysis may even be performed with reducing agents in the buffer (eg. DTT), but this will make your lysates unsuitable for BCA assay.3) Lysing samples rapidly ensures that the samples are instantly homogenized (it also shears DNA released by the SDS).5) Flash-freezing samples in liquid nitrogen rather than freezing at -80*C reduces the window of time for protease activity.6) Freeze samples in individual aliquots, instead of thawing the same vial multiple times.
I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
- CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
I am curious to know the biochemical reactions of CoCl2 that mimic hypoxia. Is it that CoCl2 can bind any ubiquitin enzyme which regulates their degradation?
- CoCl2 inhibits PHD enzymes (the body’s “oxygen sensors”) by replacing the Fe ion with Co, preventing these enzymes from marking HIF-1 alpha for degradation. CoCl2-based hypoxia mimetic samples are often used as positive control in HIF analysis. For more troubleshooting tips and frequently asked questions regarding hypoxia/HIFs, you can refer to our hypoxia-related FAQs.
Is cross-reactivity with HIF-2 alpha tested/predicted?
- Although we don’t have cross-reactivity data with regards to HIF-2 alpha, we predict minimal cross-reactivity based on low sequence similarity observed from BLAST analysis between HIF-1 alpha and HIF-2 alpha.
Do you have any specific protocol or relative literature for HIF1a FACS staining?
- This particular antibody has not been tested in house, so unfortunately we do not have a specific protocol for this. We have a collaborator who tested this antibody in FACS and that image is on our datasheet. With the image is a brief of their protocol: 'Flow Cytometry: HIF-1 alpha Antibody [NB100-449] - Hela cells were treated for 15 hrs with 200uM CoCl2, fixed in PFA, and permeabilized in 90% MeOH. 1 X 10^6 cells were stained with 0.125ug anti- HIF-alpha and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Black- treated, anti-KLH control IgG; Red- untreated, anti-HIF1-alpha; Blue- treated, anti-HIF1-alpha.'
I detect the 60 kDa band for HIF-1 alpha antibody (NB100-449), but no band at the right molecular weight. Do you know what this non-specific band is?
- Most likely the 60kDa band is a degradation product of HIF1A in your samples. Since HIF-1 alpha degrades so rapidly in the presence of oxygen, our lab often sees bands at lower molecular weights. We have also experienced some non-specific banding around that molecular weight too in some tests.
Could you tell us if these antibodies need to use cell lysate treated with special extraction for WB? Do you have the recommended preparation method of cell lysate?
- Usually HIF1 alpha and HIF2 alpha are hard to detect endogenously and require some inducement. The most common is hypoxic treatment of the cells. This can be also be chemically induced by CoCl2 treatment as well. Our recommended protocols and lysate preparations can be found in our white paper here

Show All 14 FAQs.

Control Lysate(s)

Human Brain Cerebral Cortex Whole Tissue Lysate (Adult Whole Normal)
Human Pancreas Whole Tissue Lysate (Adult Whole Tumor)
COS-7 Nuclear Hypoxic Induced Cell Lysate
HeLa CoCl2 treated/Untreated Cell Lysate
HepG2 CoCl2 treated/Untreated Cell Lysate
HeLa Hypoxic / Normoxic Cell Lysate
HepG2 Hypoxic / Normoxic Cell Lysate
HIF-1 alpha Knockout CoCl2-treated/untreated HeLa Cell Lysate
HIF-1 alpha Knockout Hypoxic-treated/untreated HeLa Cell Lysate
NIH 3T3 CoCl2 treated/Untreated Cell Lysate
L929 CoCl2 treated/Untreated Cell Lysate
Raw 264.7 CoCl2 treated/Untreated Cell Lysate
PC12 CoCl2 treated/Untreated Cell Lysate
PC12 Hypoxic / Normoxic Cell Lysate
Raw 264.7 Hypoxic / Normoxic Cell Lysate
L929 Hypoxic / Normoxic Cell Lysate
NIH 3T3 Hypoxic / Normoxic Cell Lysate

Secondary Antibodies

Isotype Controls

Additional HIF-1 alpha Products

Research Areas for HIF-1 alpha Antibody (NB100-449)

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Blogs on HIF-1 alpha. Showing 1-10 of 37 blog posts - Show all blog posts.

Check out the latest blog posts on HIF-1 alpha.

Hypoxia-Dependent CAR Stabilizing Construct in T cells Improves Solid Tumor Targeting and Efficacy
By Victoria Osinski, PhDDespite advances in the development of cancer immunotherapies, those specifically targeting tumors still remains limited. Currently, there is great interest in utilizing chimeric antigen rece... Read full blog post.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ... Read full blog post.

Understanding ‘Y’ in Breast Cancer: Crucial Role of DNA/RNA-binding Protein YB-1 in the Development, Pre-Invasive, and Metastatic Phases
Jamshed Arslan, Pharm D, PhD In the United States, 1 in 8 women will be diagnosed with breast cancer in her lifetime.1 Despite the prevalence, cancer genesis is a mystery. The heterogeneity of cancers makes it diff... Read full blog post.

Read full blog post.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax... Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ... Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ... Read full blog post.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto... Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy... Read full blog post.

Forecasting and Targeting a Rare Cancer with Hypoxia-Inducible Factor
By Jamshed Arslan Pharm.D. Cancers of nerve, adipose, and other soft tissues are called soft tissue sarcomas (STS). Malignant peripheral nerve sheath tumor (MPNST) is an example of a rare and hard-to-treat STS; eve... Read full blog post.

Showing 1-10 of 37 blog posts - Show all blog posts.

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	Verified Customer 01/11/2024
Application:	WB
Species:	Mouse

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	Verified Customer 05/08/2020
Application:	WB
Species:	Mouse

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	Verified Customer 06/07/2019
Application:	IHC-P
Species:	Mouse

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Bioinformatics

Gene Symbol	HIF1A
Entrez	3091(Human) 15251(Mouse)
Uniprot	Q16665()

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

HIF-1 alpha Antibody

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FAQs for HIF-1 alpha Antibody (NB100-449). (Showing 1 - 10 of 14 FAQs).

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Additional HIF-1 alpha Products

Research Areas for HIF-1 alpha Antibody (NB100-449)

Blogs on HIF-1 alpha. Showing 1-10 of 37 blog posts - Show all blog posts.

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