Hexosaminidase A/HEXA Antibody - BSA Free

Images

 
Western Blot: Hexosaminidase A/HEXA Antibody [NBP1-74127] - Titration: 1 ug/ml Positive Control: Fetal kidney lysate.
Immunohistochemistry-Paraffin: Hexosaminidase A/HEXA Antibody [NBP1-74127] - Human Brain, cortex tissue at an antibody concentration of 5ug/ml.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

Hexosaminidase A/HEXA Antibody - BSA Free Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to the C terminal of HEXA. Immunizing peptide sequence: WKDFYIVEPLAFEGTPEQKALVIGGEACMWGEYVDNTNLVPRLWPRAGAV. The peptide sequence for this immunogen was taken from within the described region.
Specificity
This product is specific to Subunit or Isoform: alpha.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HEXA
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Western Blot 1.0 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID 27466344).
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP1-74127 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 27466344).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Hexosaminidase A/HEXA Antibody - BSA Free

  • beta-hexosaminidase subunit alpha
  • Beta-N-acetylhexosaminidase subunit alpha
  • EC 3.2.1
  • EC 3.2.1.52
  • HEXA
  • hexosaminidase A (alpha polypeptide)
  • Hexosaminidase A
  • Hexosaminidase subunit A
  • MGC99608
  • N-acetyl-beta-glucosaminidase subunit alpha
  • TSD

Background

This gene encodes the alpha subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Alpha subunit gene mutations lead to Tay-Sachs disease (GM2-gangliosidosis type I).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Hexosaminidase A/HEXA Antibody (NBP1-74127)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
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Filter By Species
Rat
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HEXA
Uniprot