![Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HE4-WFDC2-Antibody-JF62-09-Immunohistochemistry-Paraffin-NBP2-66883-img0002.jpg "Immunohistochemistry-Paraffin: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Analysis of paraffin-embedded mouse lung tissue using anti-HE4 antibody. Counter stained with hematoxylin.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | JF62-09 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic peptide within Human HE4/WFDC2 aa 78-114 / 124. (SwissProt: Q14508 Human; SwissProt: Q9DAU7 Mouse) |
| Localization | Secreted. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | WFDC2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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| Publication using NBP2-66883 | Applications | Species |
|---|---|---|
| Liu L, Ma F, Hao Y et al. Integrative Informatics Analysis of Transcriptome and Identification of Interacted Genes in the Glomeruli and Tubules in CKD Frontiers in Medicine 2021-02-12 [PMID: 33644086] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for HE4/WFDC2 Antibody (NBP2-66883)Find related products by research area.
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| Gene Symbol | WFDC2 |
![Flow Cytometry: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Flow cytometric analysis of SW480 cells with HE4/WFDC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/HE4-WFDC2-Antibody-JF62-09-Flow-Cytometry-NBP2-66883-img0005.jpg "Flow Cytometry: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - Flow cytometric analysis of SW480 cells with HE4/WFDC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Western Blot: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - analysis of HE4 on different lysates with Rabbit anti-HE4 antibody (ET1702-61) at 1/2,000 dilution.Lane 1: SW480 cell lysate (15 ug/Lane)Lane 2: HepG2 cell lysate (15 ug/Lane)Lane 3: OVCAR-3 cell lysate (15 ug/Lane)Lane 4: Mouse lung tissue lysate (20 ug/Lane)Lane 5: Mouse kidney tissue lysate (20 ug/Lane)Predicted band size: 13 kDaObserved band size: 20 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-66883_rabbit-monoclonal-he4-wfdc2-antibody-jf62-09-western-blot-1062024131123.jpg "Western Blot: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - analysis of HE4 on different lysates with Rabbit anti-HE4 antibody (ET1702-61) at 1/2,000 dilution.Lane 1: SW480 cell lysate (15 ug/Lane)Lane 2: HepG2 cell lysate (15 ug/Lane)Lane 3: OVCAR-3 cell lysate (15 ug/Lane)Lane 4: Mouse lung tissue lysate (20 ug/Lane)Lane 5: Mouse kidney tissue lysate (20 ug/Lane)Predicted band size: 13 kDaObserved band size: 20 kDaExposure time: 1 minute;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - analysis of HepG2 cells labeling HE4 with Rabbit anti-HE4 antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HE4 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4c. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66883_rabbit-monoclonal-he4-wfdc2-antibody-jf62-09-immunocytochemistry-immunofluorescence-1062024131544..jpg "Immunocytochemistry/Immunofluorescence: HE4/WFDC2 Antibody (JF62-09) [NBP2-66883] - analysis of HepG2 cells labeling HE4 with Rabbit anti-HE4 antibody at 1/100 dilution.Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HE4 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin ( red) was stained at 1/100 dilution overnight at +4c. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")