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HCLS1 Antibody

Images

 
Western Blot: HCLS1 Antibody [NBP1-30953] - Sample (30 ug of whole cell lysate) A: Jurkat B: HL-60 7. 5% SDS PAGE; antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: HCLS1 Antibody [NBP1-30953] - Paraffin-embedded Lymphocytes in gastric epithelium, using antibody at 1:100 dilution.
Western Blot: HCLS1 Antibody [NBP1-30953] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, PmSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HCLS1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HCLS1. The exact sequence is proprietary.
Localization
Membrane, Peripheral membrane protein (Probable) , Cytoplasm (Probable) , Mitochondrion
Predicted Species
Rat (100%), Chicken (92%), Monkey (100%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HCLS1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for HCLS1 Antibody

  • Cortactin-like
  • CTTNL
  • HCLS1
  • hematopoietic cell-specific Lyn substrate 1hematopoietic lineage cell-specific protein
  • HS1lckBP1
  • LckBP1
  • p75

Background

Hematopoietic cell specific Lyn substrate 1 (HCLS1) is an intracellular protein involved in the signal transduction pathways in B and T lymphocytes. HCLS1 Tyr phosphorylation catalyzed by Syk and Lyn plays a crucial role in the translocation of the protein to the membrane and is involved in the cytoskeleton rearrangement triggered by thrombin in human platelets. The gene is a substrate for caspase cleavage during apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Array NBP1-30953

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Bioinformatics

Gene Symbol HCLS1