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Haptoglobin Antibody

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Western Blot: Haptoglobin Antibody [NBP1-32391] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Haptoglobin antibody diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Haptoglobin Antibody [NBP1-32391] - Human plasma (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Haptoglobin antibody diluted at 1:30000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: Haptoglobin Antibody [NBP1-32391] - Paraffin-embedded Normal gastric, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Pm, RMSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Haptoglobin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Haptoglobin. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Rhesus Macaque (94%), Primate (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HP
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:30000
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (98%), Mouse (81%), Rat (80%), Cat (83%), Pig (81%), Rabbit (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for Haptoglobin Antibody

  • binding peptide
  • BP
  • haptoglobin alpha(1S)-beta
  • haptoglobin alpha(2FS)-beta
  • Haptoglobin
  • haptoglobin, alpha polypeptide
  • haptoglobin, beta polypeptide
  • HP
  • HP-1
  • hp2-alpha
  • HP2ALPHA2
  • HPA1S
  • MGC111141
  • zonulin

Background

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Haptoglobin Antibody (NBP1-32391). (Showing 1 - 1 of 1 FAQ).

  1. Is this antibody specific for zonulin (pre-haptoglobin 2)?
    • Our haptoglobin antibody with catalogue number NBP1-32391 was raised to a recombinant fragment corresponding to a region within amino acids 57 and 406 of the human haptoglobin protein according to the following accession on: Uniprot. Un-cleaved haptoglobin, also known as zonulin, is processed to give rise to the alpha and beta haptoglobin chains. The alpha chain consists of amino acids 19-160, while the beta chain consists of amino acids 162-406. As the immunogen region for NBP1-32391 spans both of these chains, the antibody should recognise zonulin, although as we don't routinely epitope map our antibodies I cannot tell you exactly where on the haptoglobin protein the binding takes place.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HP