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Haptoglobin Antibody (448018) [Unconjugated]

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Recombinant Human Haptoglobin protein was serially diluted 2-fold and captured by Mouse Anti-Human Haptoglobin Monoclonal Antibody (Catalog # MAB8465) coated on a Clear Polystyrene Microplate (Catalog # DY990).Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant Human Haptoglobin protein was serially diluted 2-fold and captured by Mouse Anti-Human Haptoglobin Monoclonal Antibody (Catalog # MAB8465) coated on a Clear Polystyrene Microplate (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
448018
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB8465-SP
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Haptoglobin Antibody (448018) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Haptoglobin
Met1-Gln347
Accession # P00738
Specificity
Detects human Haptoglobin in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Haptoglobin Antibody (448018) [Unconjugated]

  • binding peptide
  • BP
  • haptoglobin alpha(1S)-beta
  • haptoglobin alpha(2FS)-beta
  • Haptoglobin
  • haptoglobin, alpha polypeptide
  • haptoglobin, beta polypeptide
  • HP
  • HP-1
  • hp2-alpha
  • HP2ALPHA2
  • HPA1S
  • MGC111141
  • zonulin

Background

Haptoglobin is a 406 aminoacids (aa) protein that in humans is encoded by the HP gene. This gene encodes a preproprotein that is processed into alpha (aa 19-160) and beta (aa 162-406) chains that combine as a tetramer to produce haptoglobin. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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