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Recombinant Human HAI-2/SPINT2 His Protein

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SDS-Page: SPINT2 Protein [NBP2-23460]
SDS-Page: SPINT2 Protein [NBP2-23460]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

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Recombinant Human HAI-2/SPINT2 His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 28-197 of Human HAI-2/SPINT2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMADRER SIHDFCLVSK VVGRCRASMP RWWYNVTDGS CQLFVYGGCD GNSNNYLTKE ECLKKCATVT ENATGDLATS RNAADSSVPS APRRQDSEDH SSDMFNYEEY CTANAVTGPC RASFPRWYFD VERNSCNNFI YGGCRGNKNS YRSEEACMLR CFRQQENPPL PLGSK
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
SPINT2
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
21.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human HAI-2/SPINT2 His Protein

  • HAI2
  • HAI-2
  • HAI-2DIAR3
  • HAI2FLJ45571
  • Hepatocyte growth factor activator inhibitor type 2
  • Kop
  • kunitz-type protease inhibitor 2
  • PB
  • Placental bikunin
  • serine peptidase inhibitor, Kunitz type, 2
  • serine protease inhibitor, Kunitz type, 2

Background

SPINT2 is a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. This protein is a putative tumor suppressor, and mutations in this protein result in congenital sodium diarrhea. Recombinant human SPINT2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol SPINT2