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GPVI Antibody (784808) [Unconjugated]

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Mouse platelets were stained with Rat Anti-Mouse GPVI Monoclonal Antibody (Catalog # MAB6758, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Mouse platelets were stained with Rat Anti-Mouse GPVI Monoclonal Antibody (Catalog # MAB6758, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated ...read more
GPVI was detected in immersion fixed adult mouse platelets using Rat Anti-Mouse GPVI Monoclonal Antibody (Catalog # MAB6758) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
784808
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB6758U-100UG
Alexa Fluor 405
FAB6758V-100UG
Alexa Fluor 488
FAB6758G-100UG
Alexa Fluor 532
FAB6758X
Alexa Fluor 594
FAB6758T-100UG
Alexa Fluor 647
FAB6758R-100UG
Alexa Fluor 700
FAB6758N-100UG
Alexa Fluor 750
FAB6758S-100UG
Allophycocyanin
FAB6758A
Allophycocyanin/Cy7
FAB6758APCCY7
Biotin
FAB6758B
CoraFluor 1
FAB6758CL1
DyLight 350
FAB6758D
DyLight 405
FAB6758E
DyLight 488
FAB6758K
DyLight 550
FAB6758L
DyLight 594
FAB6758M
DyLight 650
FAB6758W
DyLight 680
FAB6758Y
DyLight 755
FAB6758Z
FITC
FAB6758F
HRP
FAB6758H
Janelia Fluor 525
FAB6758JF525
Janelia Fluor 549
FAB6758I
Janelia Fluor 646
FAB6758J
Janelia Fluor 669
FAB6758JF669
PE/Atto594
FAB6758PEATT594
PE/Cy5.5
FAB6758PECY55
PE/Cy7
FAB6758PECY7
PerCP
FAB6758C
Phycoerythrin
FAB6758P
mFluor Violet 450 SE
FAB6758MFV450
mFluor Violet 500 SE
FAB6758MFV500
mFluor Violet 610 SE
FAB6758MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB6758P
Carrier Free
MAB6758-SP
Carrier Free
FAB6758V-100UG
Carrier Free
FAB6758U-100UG
Carrier Free
FAB6758T-100UG
Carrier Free
FAB6758S-100UG
Carrier Free
FAB6758R-100UG
Carrier Free
FAB6758N-100UG
Carrier Free
FAB6758G-100UG
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GPVI Antibody (784808) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GPVI
Gly24-Lys265
Accession # P0C191
Specificity
Detects mouse GPVI in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
GP6
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using MAB6758.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPVI Antibody (784808) [Unconjugated]

  • Glycoprotein 6
  • glycoprotein VI (platelet)
  • GP6
  • GPIV
  • GPVI
  • GPVIplatelet collagen receptor
  • MGC138168
  • platelet glycoprotein VI

Background

GPVI (Platelet Glycoprotein VI; also glycoprotein 5) is a member of the Ig superfamily. It is found on platelets and megakaryocytes, and serves as the main collagen receptor on platelets. Following exposure to subendothelial connective tissue, GPVI binds to a Gly-Pro-(hydroxy)Pro motif on collagen and generates a noncovalent membrane signaling complex with FcR gamma -chain. This interaction is stabilized by Integrin alpha 2 beta 1, followed by activation of PLC gamma 2 with clot initiation. Mature mouse GPVI is a 292 amino acid (aa) type I transmembrane protein. It possesses a 244 aa extracellular region (aa 22-265) that contains two C2-type Ig-like domains (aa 27-197)and two potential glycosylation sites, plus a 37 aa cytoplasmic tail (aa 287-313). There is one potential splice form that shows a deletion of aa 224-240. Over aa 24-265, mouse GPVI shares 70% and 86% aa identity with human and rat GPVI, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GPVI Antibody
(784808) [Unconjugate
MAB6758
GPVI Antibody (784808) [Unconjugated]
Species: Mu
Applications: Flow, CyTOF-ready, ICC/IF

Publications for GPVI Antibody (MAB6758)(1)


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Bioinformatics

Gene Symbol GP6
Uniprot