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GPR64 Antibody

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Western Blot: GPR64 Antibody [NBP2-55504] - Analysis in human cell line A-549 and human cell line A-431.
Orthogonal Strategies: Immunohistochemistry-Paraffin: GPR64 Antibody [NBP2-55504] - Staining in human epididymis and endometrium tissues using NBP2-55504 antibody. Corresponding ADGRG2 RNA-seq data are presented ...read more
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP2-55504] - Staining of human endometrium shows no positivity in glandular cells as expected.
Independent Antibodies: Immunohistochemistry-Paraffin: GPR64 Antibody [NBP2-55504] - Staining of human endometrium, epididymis, liver and skeletal muscle using Anti-ADGRG2 antibody NBP2-55504 (A) shows similar ...read more
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP2-55504] - Staining of human epididymis shows strong positivity in microvilli in glandular cells.
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP2-55504] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP2-55504] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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GPR64 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: NASGVKPQRNICNLSSICNDSAFFRGEIMFQYDKESTVPQNQHITNGTLTGVLSLSELKRSELNKTLQTLSETYFIMCATAEAQSTLNCTFTIKLNNTMNACAVIAALERVKIRPMEHCCCSVRIPCP
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADGRG2
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
GPR64 Recombinant Protein Antigen (NBP2-55504PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for GPR64 Antibody

  • EDDM6
  • epididymis-specific (seven transmembrane family)
  • G protein-coupled receptor 64
  • GPR64
  • HE6
  • MGC104454
  • MGC138738
  • TM7LN2

Background

GPR64 is an Orphan-B GPCR with an unknown ligand. HE6 is endogenously cleaved into two pieces at the GPCR proteolytic site (GPS) located adjacent to the first transmembrane helix as a mechanism to compartmentalize the cell adhesion and GPCR activation functions. GPR64 has been reported in the epididymis. ESTs have been isolated from embryo, kidney, placenta, skeletal muscle, and testis libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ADGRG2