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Glycophorin A Antibody (GYPA/1725R) - Azide and BSA Free

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Western Blot: Glycophorin A Antibody (GYPA/1725R) - Azide and BSA Free [NBP2-54470] - Western Blot Analysis of K562 cell lysate using Glycophorin A Rabbit Recombinant Monoclonal Antibody (GYPA/1725R).
Immunohistochemistry-Paraffin: Glycophorin A Antibody (GYPA/1725R) - Azide and BSA Free [NBP2-54470] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Recombinant Rabbit Monoclonal Antibody (GYPA/1725R)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
GYPA/1725R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Glycophorin A Antibody (GYPA/1725R) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified by Protein A. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53136).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant full-length human Glycophorin A protein (Uniprot: P02724)
Localization
Cell surface
Marker
Erythrocyte Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
GYPA
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Glycophorin A Antibody (GYPA/1725R) - Azide and BSA Free

  • CD235a antigen
  • CD235a
  • glycophorin A (includes MN blood group)
  • glycophorin A (MNS blood group)
  • Glycophorin A
  • glycophorin Erik
  • glycophorin MiI
  • glycophorin MiIII
  • glycophorin MiV
  • glycophorin MiX
  • glycophorin SAT
  • glycophorin Sta type C
  • glycophorin-A
  • GPA
  • GPErik
  • GpMiIII
  • GPSAT
  • GYPA
  • HGpMiIII
  • HGpMiV
  • HGpMiX
  • HGpMiXI
  • HGpSta(C)
  • Mi.V glycoprotein (24 AA)
  • MN sialoglycoprotein
  • MNS
  • PAS-2
  • recombinant glycophorin A-B Miltenberger-DR

Background

Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GYPA