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Mouse Glut3 ELISA Kit (Colorimetric)

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ELISA: Mouse Glut3 ELISA Kit (Colorimetric) [NBP2-82172] - Samples were spiked with high concentrations of Mouse Glut3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Mouse Glut3 ELISA Kit (Colorimetric) [NBP2-82172] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL

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Mouse Glut3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse GLUT3 in samples. No Significant cross-reactivity or interference between Mouse GLUT3 and analogues was observed.
Standard Curve Range
0.63 - 40 ng/mL
Sensitivity
0.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 6%
Intra-Assay
CV% < 6%
Spike Recovery
average = 92% for serum; average = 93% for EDTA plasma; average = 99% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SLC2A3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Mouse Glut3 ELISA Kit (Colorimetric)

  • FLJ90380
  • Glut3
  • GLUT-3
  • GLUT3Glucose transporter type 3, brain
  • SLC2A3
  • solute carrier family 2 (facilitated glucose transporter), member 3
  • solute carrier family 2, facilitated glucose transporter member 3

Background

Glucose is the major source of our energy and there are numerous isoforms of the glucose transporter in mammals, including Glut1, Glut2, Glut3, Glut4, Glut5, Glut6, Glut7, Glut8 and Glut9. The Glut5 gene located on the short arm of human chromosome 1 encodes a 501-amino acid facilitative glucose transporter. Glut5 mRNA is highly expressed in small intestine and to a lesser extent in kidney, skeletal muscle and adipose tissue. Glut5 plays a critical role in fructose absorption in the small intestine and its expression is highly induced when exposed to a fructose-enriched diet. Glut5 transporter expressed in human skeletal muscle is specifically localized to the plasma membrane, where it participates in regulating hexose transfer across the sarcolemma. Glut8, a novel glucose transporter-like protein, exhibits significant sequence similarity with the other members of sugar transporter family. Glut8 comprises 12 putative membrane-spanning helices and several conserved motifs, which are important for transport activity. In human tissues, Glut8 is predominantly expressed in testis and, to a lesser extent, in most other tissues including skeletal muscle, heart, small intestine and brain. In addition, the Glut8 glucose transport facilitator has a hormonally regulated testicular function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SLC2A3