![GLP1 Antibody (10) - BSA Free [NBP1-05180] - BSA Free [NBP1-05180]](http://images.novusbio.com/fullsize/GLP1-Antibody-10-BSA-Free-NBP1-05180-img0001.jpg "GLP1 Antibody (10) - BSA Free [NBP1-05180] - BSA Free [NBP1-05180]")
| Reactivity | MaSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Synthetic GLP-1(7-17) |
| Epitope | Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide. |
| Specificity | Binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36)amide and shows <0.2% cross-reactivity with GLP-1(9-36)amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4. Cross-reacts with Liraglutide. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GCG |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | ELISA: NBP1-05180 binds free GLP-1(7-37) and free GLP-1(7-36)amide in solution. NBP1-05180 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well. NBP1-05180 binds free exendin-4 in solution.. When measuring "active" GLP-1 NBP1-05180B works as a detection antibody in combination with NBP1-97413. NBP1-05180B can be used as a detection antibody in combination with NBP2-21747 as a capture antibody in order to measure non-amidated GLP-1 forms and cross-reacting about 5% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized. |
| Storage | Store at 4C in the dark. |
| Buffer | 10mM Phosphate (pH 7.4) and 0.5M NaCl |
| Preservative | 0.9% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for GLP1 Antibody (NBP1-05180)Find related products by research area.
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| Gene Symbol | GCG |
![ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - Calibration curve using NBP2-23506 as the capture antibody and NBP2-23557 as the biotinylated detection antibody. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/GLP1-Antibody-10-BSA-Free-ELISA-NBP1-05180-img0002.jpg "ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - Calibration curve using NBP2-23506 as the capture antibody and NBP2-23557 as the biotinylated detection antibody. Image using the Biotin form of this antibody.")
![ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other glucagon-related peptides that may be present. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the ABS 012 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated ABS 012-35 as the detection antibody.](http://images.novusbio.com/fullsize/GLP1-Antibody-10-BSA-Free-ELISA-NBP1-05180-img0003.jpg "ELISA: GLP1 Antibody (10) - BSA Free [NBP1-05180] - By using the above monoclonal antibodies, it is possible to measure intact exendin-4 by a sandwich ELISA which is not subject to interference from GLP-1 or other glucagon-related peptides that may be present. NBP1-05180 is used as capture antibody in combination with a biotinylated detection antibody from the ABS 012 series. The best sensitivity in an unoptimized buffer assay was obtained with biotinylated ABS 012-35 as the detection antibody.")
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