We recently purchased Recombinant Human TIM-3 Protein (NBP2-23118) and GITR/TNFRSF18 ( NBP2-52215-0.05mg). Can these be diluted with sterile Di water without harming the integrity/functionality of the proteins?

Name: Recombinant Human GITR/TNFRSF18 His Protein
Brand: Novus Biologicals
SKU: NBP2-52215
Price: 329 USD

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Recombinant Human TIM-3 Protein (NBP2-23118) and Recombinant Human GITR/TNFRSF18 Protein (NBP2-52215) have been tested in PAGE but we have not tested its bioactivity/functional aspects so far. The buffer for NBP2-23118 (20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol) contains urea and glycerol, which indicates there is the solubility/aggregation issue at the high concentration.  I anticipate that if diluting with sterile double distilled water, at the certain concentrations, the protein will not be stable and precipitated out.  Unfortunately, our lab did not measure the thresholds for the precipitations.  On the other hand, NBP2-52215 has phosphate buffered saline (pH 7.4) and 10% glycerol as the buffer, which maybe tolerable in the water dilution process; however we have not done so in the past.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	PAGE
Concentration	0.5 mg/ml

Description	A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 26-162 of Human GITR/TNFRSF18 Source:Baculovirus Amino Acid Sequence: QRPTGGPGCG PGRLLLGTGT DARCCRVHTT RCCRDYPGEE CCSEWDCMCV QPEFHCGDPC CTTCRHHPCP PGQGVQSQGK FSFGFQCIDC ASGTFSGGHE GHCKPWTDCT QFGFLTVFPG NKTHNAVCVP GSPPAEPLEH HHHHH
Source	Baculovirus
Protein/Peptide Type	Recombinant Protein
Gene	TNFRSF18
Purity	>95%, by SDS-PAGE
Endotoxin Note	< 1.0 EU per 1 microgram of protein (determined by LAL method)

Dilutions	SDS-Page
Theoretical MW	15.6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS (pH 7.4), 10% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Purity	>95%, by SDS-PAGE

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