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Human GIF ELISA Kit (Colorimetric)

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ELISA: Human GIF ELISA Kit (Colorimetric) [NBP2-68010] - Samples were spiked with high concentrations of Human GIF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of ...read more
ELISA: Human GIF ELISA Kit (Colorimetric) [NBP2-68010] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL

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Human GIF ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human GIF in samples. No significant cross-reactivity or interference between Human GIF and analogues was observed.
Standard Curve Range
3.13 - 200 ng/mL
Sensitivity
1.88 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.12%
Intra-Assay
CV% < 5.04%
Spike Recovery
88-107%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CBLIF

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human GIF ELISA Kit (Colorimetric)

  • gastric intrinsic factor (vitamin B synthesis)
  • GIF
  • IF
  • IFIntrinsic factor
  • IFMH
  • IFMHgastric intrinsic factor
  • INF
  • TCN3

Background

GIF is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol CBLIF