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Ghrelin Antibody - Azide and BSA Free

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Immunohistochemistry-Paraffin: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] - Ghrelin antibody detects secreted Ghrelin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Ghrelin ...read more
Immunohistochemistry-Paraffin: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] - Ghrelin antibody detects secreted Ghrelin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Ghrelin ...read more
Western Blot: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] - Human tissue extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Ghrelin antibody (NBP3-25481) diluted at 1:1000. The ...read more
Western Blot: Ghrelin Antibody - Azide and BSA Free [NBP3-25481] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Ghrelin antibody (NBP3-25481) diluted at 1:1000. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RMSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

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Ghrelin Antibody - Azide and BSA Free Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Ghrelin. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GHRL
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Feline (86%), Canine (86%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for Ghrelin Antibody - Azide and BSA Free

  • appetite-regulating hormone
  • Ghrelin
  • ghrelin/obestatin preprohormone
  • ghrelin/obestatin prepropeptide
  • GHRL
  • Growth hormone secretagogue
  • Growth hormone-releasing peptide
  • Motilin-related Peptide
  • MTLRPgrowth hormone secretagogue receptor ligand
  • obestatin
  • Protein M46

Background

Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Ghrelin Products

Blogs on Ghrelin.

Ghrelin: Targeting the Hunger Hormone to Combat Obesity and Type 2 Diabetes
As the hormone ghrelin is linked to appetite and weight gain, as well as impaired glucose-induced insulin secretion, there is considerable interest in this peptide as a potential drug target. Although the overall lack of success in this field has been...  Read full blog post.

Shades of Ghrelin in Weight Homeostasis
The neuropeptide and gut hormone Ghrelin is an endogenous ligand for the growth hormone (GH)-secretagogue receptor (GHSR) within the central nervous system. This pathway has received a great deal of attention and heavy study within the last decade bec...  Read full blog post.

Appetite and Energy: A Ghrelin Balancing Act
Ghrelin is the only potent orexigenic peptide in circulation. It stimulates food intake and leads to metabolism regulation, positive energy balance, adipogenesis, and body weight gain. However, in studies using ghrelin antibodies, the physiological si...  Read full blog post.

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Bioinformatics

Gene Symbol GHRL