GFR alpha-1/GDNF R alpha-1 Antibody (81401) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Rat, Rabbit, Transgenic Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-Fr, Neutralization.

Showing Publications 1 - 6 of 6.

Publications using MAB560	Applications	Species
Song, Y;Zhang, X;Desmarais, JA;Nagano, M; Postnatal development of mouse spermatogonial stem cells as determined by immunophenotype, regenerative capacity, and long-term culture-initiating ability: a model for practical applications Scientific reports 2024-01-27 [PMID: 38280889] (Flow Cytometry, Transgenic Mouse)	Flow Cytometry	Transgenic Mouse
Frank L. Rice, George Houk, James P. Wymer, Sara J. C. Gosline, Justin Guinney, Jianqiang Wu et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin PLOS ONE 2019-05-20 [PMID: 31107888]
Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM). Mol Cell Neurosci, 2012-12-20;54(0):30-43. 2012-12-20 [PMID: 23262364] (IHC-Fr, Rat)	IHC-Fr	Rat
Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties. FASEB J., 2011-04-27;25(0):2604-14. 2011-04-27 [PMID: 21525489] (Flow Cytometry, Rabbit)	Flow Cytometry	Rabbit
Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin. Biochemistry, 2005-02-22;44(7):2545-54. 2005-02-22 [PMID: 15709767] (Neutralization, Rat)	Neutralization	Rat
Leitner ML, Wang LH, Osborne PA, Golden JP, Milbrandt J, Johnson EM Expression and function of GDNF family ligands and receptors in the carotid body. Exp. Neurol., 2005-02-01;191(0):S68-79. 2005-02-01 [PMID: 15629763] (ICC, IHC, Rat)	ICC, IHC	Rat

Array MAB560

• GFR alpha-1/GDNF R alpha-1 Antibodies
• GFR alpha-1/GDNF R alpha-1 ELISA Kits
• GFR alpha-1/GDNF R alpha-1 Lysates
• GFR alpha-1/GDNF R alpha-1 Proteins
• GFR alpha-1/GDNF R alpha-1 Proteins and Enzymes