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GATA-1 Antibody

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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL of Human GATA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1779) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA-1 at approximately 50 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL of Human GATA-1 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF1779
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF1779-SP
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GATA-1 Antibody Summary

Specificity
Detects human GATA-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows less than 1% cross‑reactivity with rhGATA-2, rhGATA-5 and rhGATA-6.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GATA1
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GATA-1 Antibody

  • Eryf1
  • ERYF1GATA-binding protein 1 (globin transcription factor 1)
  • GATA binding protein 1 (globin transcription factor 1)
  • GATA1
  • GATA-1
  • GATA-1erythroid transcription factor
  • GATA-binding factor 1
  • GF-1
  • GF1globin transcription factor 1
  • NF-E1 DNA-binding protein
  • NFE1erythroid transcription factor 1
  • transcription factor GATA1
  • XLTT

Background

GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA‑1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis (1).
  1. Patient, R.K. and J. McGhee (2002) Curr. Opin. Genet. Dev. 12:416.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol GATA1
Uniprot