![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [DyLight 488] [NB300-538G] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. T98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue). Confocal immunofluorescence using DyLight 488 conjugated galectin-3 antibody. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-DyLight-488-Immunocytochemistry-NB300-538G-img0001.jpg "Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [DyLight 488] [NB300-538G] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. T98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue). Confocal immunofluorescence using DyLight 488 conjugated galectin-3 antibody. This image was submitted via customer Review.")
| Reactivity | Hu, Mu, Rt, RbSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | A3A12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | DyLight 488 |
| Immunogen | Full length recombinant human galectin-3. |
| Epitope | The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3. |
| Specificity | Galectin 3 (A3A12) |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | LGALS3 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Reviewed Applications |
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| Storage | Store at 4C in the dark. |
| Buffer | 50mM Sodium Borate |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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reviewed by:
Verified Customer |
ICC | Human | 08/31/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for Galectin-3 Antibody (NB300-538G)Find related products by research area.
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