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Galectin 13 Antibody (PP13/1161)

Images

 
Western Blot: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Western Blot of K562 Cell Lysate using Galectin 13 antibody (PP13/1161).
Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Spleen stained with Galectin-13MAb (PP13/1161).
Western Blot: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Western Blot of (A) Recombinant human Galectin-13 fragment And (B) K562 Cell Lysate using Galeactin-13 MAb (PP13/1161).
Immunohistochemistry-Paraffin: Galectin 13 Antibody (PP13/1161) [NBP2-53210] - Formalin-paraffin human Placenta stained with Galectin-13 MAb (PP13/1161).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
PP13/1161
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

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Galectin 13 Antibody (PP13/1161) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54358)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human Galectin 13 protein fragment (aa23-134) (Uniprot: Q9UHV8)
Localization
Cytoplasmic & Cell Surface
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LGALS13
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Galectin 13 Antibody (PP13/1161)

  • beta-galactoside-binding lectin
  • EC 3.1.1.5
  • GAL13
  • gal-13
  • galectin-13
  • lectin, galactoside-binding, soluble, 13
  • PLAC8galectin 13
  • Placental protein 13
  • Placental tissue protein 13
  • PP13galactoside-binding soluble lectin 13

Background

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene has lysophospholipase activity. It is composed of two identical subunits which are held together by disulfide bonds. This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LGALS13
Uniprot