GAD65/67 Antibody Summary
Immunogen |
A synthetic peptide from amino acid region 530-600 of mouse GAD65 conjugated to blue carrier protein was used as the antigen. The peptide is identical in GAD67. The antigen is homologous in rat and human. |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
GAD1 |
Purity |
Unpurified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 1:1000 - 1:2000
- Immunohistochemistry-Paraffin 1:1000 - 1:2000
- Western Blot 1:1000 - 1:2000
|
Reactivity Notes
Other species not yet tested but expected to work in zebrafish, canis familiaris, macaca mulatta, sus scrofa.
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
Lyophilized from whole serum |
Preservative |
No Preservative |
Purity |
Unpurified |
Reconstitution Instructions |
Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. |
Alternate Names for GAD65/67 Antibody
Background
GAD65/67 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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