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Frizzled-4 Antibody

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Western Blot: Frizzled-4 Antibody [NB100-93397] - (1ug/ml) staining of Small Intestine (Ileum) cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Frizzled-4 Antibody Summary

Immunogen
Peptide with sequence DEEERRCDPIRIS corresponding to internal region according to NP_036325.2.
Epitope
DEEERRCDPIRIS
Predicted Species
Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FZD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human colon and small intestine (Ileum) lysates (calculated MW of 59.9 kDa band according to NP_036325.2).
Control
Frizzled-4 Overexpression Lysate
Publications
Read Publication using NB100-93397.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Frizzled-4 Antibody

  • CD344 antigen
  • CD344
  • EVR1
  • exudative vitreoretinopathy 1
  • FEVR
  • frizzled (Drosophila) homolog 4
  • frizzled homolog 4 (Drosophila)
  • Frizzled4
  • Frizzled-4
  • Fz-4
  • FZD4
  • FZD4S
  • FzE4
  • GPCR
  • hFz4
  • MGC34390
  • WNT receptor frizzled-4

Background

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Frizzled-4 Antibody (NB100-93397)(1)


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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FZD4
Entrez
Uniprot