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FoxP3 Antibody

Images

 
Western Blot: FoxP3 Antibody [NB600-242] - (1 ug/mL) staining of Human Muscle (A), (2 ug/mL) MOLT4 (B) and (1 ug/mL) negative control Pancreas (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry: FoxP3 Antibody [NB600-242] - (Treg) human blood cells gathered by cytospin and detected by FITC (A) and in phase contrast (B).
Immunohistochemistry: FoxP3 Antibody [NB600-242] - (8ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
Flow Cytometry: FoxP3 Antibody [NB600-242] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody ...read more
Immunofluorescence: FoxP3 Antibody [NB600-242] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing ...read more
Control: FoxP3 Antibody [NB600-242] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, Ctrl
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FoxP3 Antibody Summary

Immunogen
Peptide with sequence SQRPSRCSNPTPGP corresponding to C-Terminus according to NP_054728.2, NP_001107849.1.
Localization
Nuclear (potential).
Specificity
This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1)
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Control
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin 6.0 - 8.0 ug/ml
  • Peptide ELISA 1:8000
  • Western Blot 1 - 3 ug/mL
Application Notes
Western blot: Approx 48-50kDa band observed in Human Muscle lysates and in lysates of cell line MOLT4 (calculated MW of 47.2kDa according to NP_054728.2). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate
Control
FoxP3 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB600-242 in the following applications:

Publications
Read Publications using
NB600-242 in the following applications:

Reactivity Notes

Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FoxP3 Antibody

  • AIID
  • AIIDMGC141961
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxP3 Antibody (NB600-242)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, IF/IHC.


Filter By Application
ICC/IF
(1)
IF/IHC
(3)
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(2)
Mouse
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB600-242 Applications Species
Pilichowska MD, Gupta GK Immunohistochemical Expression of Vitamin D Receptor and Forkhead Box P3 in Classic Hodgkin Lymphoma: Correlation with Clinical and Pathologic Findings Oncology 2019-11-20 [PMID: 32513132]
Gupta, G K, Agrawal, T Et al. Immunohistochemical expression of vitamin D receptor and forkhead box P3 in classic Hodgkin lymphoma: correlation with clinical and pathologic findings. BMC Cancer 2020-06-08 [PMID: 32513132] (IF/IHC, Mouse) IF/IHC Mouse
Turnquist HR, Zhao Z, Rosborough BR et al. IL-33 Expands Suppressive CD11b+ Gr-1int and Regulatory T Cells, including ST2L+ Foxp3+ Cells, and Mediates Regulatory T Cell-Dependent Promotion of Cardiac Allograft Survival. Journal of Immunology 2011-09-26 [PMID: 21949025]
Petrilli G, Lorenzi L, Paracchini R et al. Epstein-Barr Virus-Associated Adrenal Smooth Muscle Tumors and Disseminated Diffuse Large B-Cell Lymphoma in a Child With Common Variable Immunodeficiency: A Case Report and Review of the Literature. Int J Surg Pathol. 2011-03-31 [PMID: 21454372] (IF/IHC, Human) IF/IHC Human
Shen S, Shin JJ, Strle K et al. Treg cell numbers and function in patients with antibiotic-refractory or antibiotic-responsive Lyme arthritis. Arthritis Rheum 2010-07-01 [PMID: 20506317] (IF/IHC, ICC/IF, Human) IF/IHC, ICC/IF Human
Stahl HF et al. miR-155 inhibition sensitizes CD4+ Th cells for TREG mediated suppression. PLoS One;4(9):e7158. 2009-09-24 [PMID: 19777054]
Mansour H, Homs S, Desvaux D, Badoual C, Dahan K, Matignon M, Audard V, Lang P, Grimbert P. Intragraft levels of Foxp3 mRNA predict progression in renal transplants with borderline change. J Am Soc Nephrol;19(12):2277-81. 2008-12-01 [PMID: 18667728]
Radulovic S, Jacobson MR, Durham SR, Nouri-Aria KT. Grass pollen immunotherapy induces Foxp3-expressing CD4+ CD25+ cells in the nasal mucosa. J Allergy Clin Immunol;121(6):1467-72, 1472. 2008-06-01 [PMID: 18423565]
Banham AH, Brown PJ, Lyne L, Schulze HJ, Hallermann C. Is FOXP3 expressed in cutaneous T-cell lymphomas? . Eur J Haematol;80(1):90-1. 2008-01-01 [PMID: 18036183]
Ward FJ, Hall AM, Cairns LS, Leggat AS, Urbaniak SJ, Vickers MA, Barker RN. Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia. Blood15;111(2):680-7. 2008-01-01 [PMID: 17761830]
Show All 12 Publications.

Review for FoxP3 Antibody (NB600-242) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB600-242:
Filter by Applications
WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Western Blot FoxP3 NB600-242
Enlarge
5
reviewed by:
Luiz Alexandre Magno
WB Rat 10/26/2018
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Summary

ApplicationWestern Blot
Sample TestedPC12 cell whole lysate
SpeciesRat
Lot51006

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB600-242). (Showing 1 - 3 of 3 FAQs).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence within the C-terminal region (SQRPSRCSNPTPGP) of FoxP3.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional FoxP3 Products

Research Areas for FoxP3 Antibody (NB600-242)

Find related products by research area.

Blogs on FoxP3.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

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Recent Reviews

5
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1
4
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2
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1
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Luiz Alexandre Magno
10/26/2018
Application: WB
Species: Rat

Bioinformatics

Gene Symbol FOXP3
Entrez
Uniprot