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FGFR1 Antibody (M17A3) - BSA Free

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Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-FGFR1 in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Western Blot: FGF R1 Antibody (M17A3) [NB100-2079] - Whole cell protein from HepG2 (lane 1), 3T3 (lane 2) and PC12 (lane 3) was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunohistochemistry-Paraffin: FGF R1 Antibody (M17A3) [NB100-2079] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human normal skin using mouse monoclonal FGF R1 Antibody (clone M17A3) at 5 ug/ml ...read more
Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in HEK293 cells (2x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat ...read more
Flow Cytometry: FGF R1 Antibody (M17A3) [NB100-2079] - Analysis of FGF R1 in MCF-7 cells (1x10^6 cells/ml) were stained with FGF R1 antibody (NB100-2079, red) at 1:1000 dilution. Detected with FITC conjugated goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, IP, CyTOF-ready
Clone
M17A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2079AF350
Alexa Fluor 405
NB100-2079AF405
Alexa Fluor 488
NB100-2079AF488
Alexa Fluor 532
NB100-2079AF532
Alexa Fluor 594
NB100-2079AF594
Alexa Fluor 647
NB100-2079AF647
Alexa Fluor 700
NB100-2079AF700
Alexa Fluor 750
NB100-2079AF750
Allophycocyanin
NB100-2079APC
Biotin
NB100-2079B
CoraFluor 1
NB100-2079CL1
DyLight 350
NB100-2079UV
DyLight 405
NB100-2079V
DyLight 488
NB100-2079G
DyLight 550
NB100-2079R
DyLight 594
NB100-2079DL594
DyLight 650
NB100-2079C
DyLight 680
NB100-2079FR
DyLight 755
NB100-2079IR
FITC
NB100-2079F
HRP
NB100-2079H
Janelia Fluor 525
NB100-2079JF525
Janelia Fluor 549
NB100-2079JF549
Janelia Fluor 585
NB100-2079JF585
Janelia Fluor 635
NB100-2079JF635
Janelia Fluor 646
NB100-2079JF646
Janelia Fluor 669
NB100-2079JF669
PE
NB100-2079PE
PerCP
NB100-2079PCP
mFluor Violet 450 SE
NB100-2079MFV450
mFluor Violet 500 SE
NB100-2079MFV500
mFluor Violet 610 SE
NB100-2079MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NB100-2079SS
Azide and BSA Free
NBP2-80732
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FGFR1 Antibody (M17A3) - BSA Free Summary

Immunogen
Recombinant human ectodomain of FGF R1 expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
Epitope
Epitope is undetermined but presumed to be near junction of the common FGF R1 NH2-terminus and NH2-terminus of Ig loop II.
Localization
Cell Membrane
Specificity
Reacts with beta isoform of FRFR1. Western blotting: at 1 ug/ml recognizes 10 ng recombinant bacterial, baculoviral, or native FGFr1. Immunoprecipitation: native, nuclear, recombinant, and ligand-labeled FGFr1. Immunohistochemistry: under investigation.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
FGFR1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:500-1:1000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 2-4 ug/ml
Application Notes
This antibody is Cytof ready.
Publications
Read Publications using NB100-2079.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FGFR1 Antibody (M17A3) - BSA Free

  • basic fibroblast growth factor receptor 1
  • BFGFR
  • bFGF-R-1
  • CD331 antigen
  • CD331
  • CEK
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R1
  • FGFBR
  • FGFR1
  • FGFR-1
  • fibroblast growth factor receptor 1
  • FLGH3
  • FLJ99988
  • Flt-2
  • FLT2H4
  • Fms-like tyrosine kinase 2
  • fms-related tyrosine kinase 2
  • H2
  • H5
  • HBGFR
  • heparin-binding growth factor receptor
  • hydroxyaryl-protein kinase
  • KAL 2
  • KAL2
  • N-SAM
  • OGD
  • Proto-oncogene c-Fgr
  • soluble FGFR1 variant 1
  • soluble FGFR1 variant 2

Background

FGF R1 (fibroblast growth factor receptor 1) is a member of the fibroblast growth factor receptor family containing an Ig-like domain and a tyrosine kinase domain. This receptor has multiple isoforms and is a Type I membrane protein. FGF R1 is a widely expressed membrane receptor, with distinct isoforms expressed in specific tissues. FGF R1 binds fibroblast growth factor and induces mitogenesis and cellular differentiation. Defects in FGF R1 result in Pfeiffer syndrome associated with craniosynostosis. FGF R1 can be modified by phosphorylation and can bind basic/acidic fibroblast factor depending on the receptor isoform. FGF R1 has been shown to interact with N-cadherin and NCAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGFR1 Antibody (NB100-2079)(2)

We have publications tested in 1 application: WB.


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Product General Protocols

View specific protocols for FGFR1 Antibody (NB100-2079): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for FGFR1 Antibody (NB100-2079). (Showing 1 - 3 of 3 FAQs).

  1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
    • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
  2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
    • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.
  3. Do you have any information on these questions regarding the flow picture on the product's datasheet? 1) Is the epitope extracellular? 2) Were the cells permeabilized before staining? 3) Is there any literature about this antibody and flow cytometry?
    • In answer to your questions: 1) The epitope is undetermined but presumed to be near junction of the common FGFr1 NH2-terminus and NH2-terminus of Ig loop II. 2) The immunogen was made to the extracellular domain, so permeabilization was not performed. 3) We unfortunately do not have any publications with this particular antibody.

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Bioinformatics

Gene Symbol FGFR1
Entrez
Uniprot