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Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

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ELISA: Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric) [NBP3-00384] - Samples were spiked with high concentrations of Human Fc gamma RIIIB/CD16b and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric) [NBP3-00384] - Samples were spiked with high concentrations of Human Fc gamma RIIIB/CD16b and diluted with Reference Standard & Sample Diluent to produce ...read more
ELISA: Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric) [NBP3-00384] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

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Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Fc gamma RIIIB/CD16b in samples. No significant cross-reactivity or interference between Human Fc gamma RIIIB/CD16b and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
4.71%
Intra-Assay
5.37%
Spike Recovery
85 - 104%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
FCGR3B

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric)

  • CD16
  • CD16b antigen
  • CD16b
  • Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
  • Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
  • Fc gamma RIIIB
  • FCG3
  • Fc-gamma receptor IIIb (CD 16)
  • Fc-gamma RIII
  • Fc-gamma RIIIb
  • Fc-gamma RIII-beta
  • FCGR3
  • FCGR3B
  • FcgRIIIB
  • FcR-10
  • fcRIII
  • FCRIIIB
  • IGFR3
  • IgG Fc receptor III-1
  • low affinity immunoglobulin gamma Fc region receptor III-B

Background

CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product PDFs

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  Human Fc gamma RIIIB/CD16b ELISA Kit (Colorimetric) Manual
  PDF Datasheet
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Bioinformatics

Gene Symbol FCGR3B