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Fc gamma RIIIB/CD16b Antibody (24) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
24
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

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Fc gamma RIIIB/CD16b Antibody (24) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Fc gamma RIIIB/CD16b (Uniprot#: O75015.2; Met1-Ser200).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FCGR3B
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Fc gamma RIIIB/CD16b Antibody (24) [CoraFluor™ 1]

  • CD16
  • CD16b antigen
  • CD16b
  • Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
  • Fc fragment of IgG, low affinity IIIb, receptor for (CD16)
  • Fc gamma RIIIB
  • FCG3
  • Fc-gamma receptor IIIb (CD 16)
  • Fc-gamma RIII
  • Fc-gamma RIIIb
  • Fc-gamma RIII-beta
  • FCGR3
  • FCGR3B
  • FcgRIIIB
  • FcR-10
  • fcRIII
  • FCRIIIB
  • IGFR3
  • IgG Fc receptor III-1
  • low affinity immunoglobulin gamma Fc region receptor III-B

Background

CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol FCGR3B