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Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free

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Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/CD32 antibody NBP3-08984 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD16/CD32-positive population is apparent.
Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/CD32 antibody NBP3-08984 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody ...read more
Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IP, B/N
Clone
2.4G2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP3-08984-0.025mg
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Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Mouse BALB/c Macrophage J774 Cell Line.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Rat
Gene
FCGR3A
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies made by Absolute Antibody that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free

  • CD16;CD16A;CD16-II;FCG3;FCGR3;FCGR3A;FCGRIII;FcGRIIIA;FCR-10;FCRIII;FCRIIIA;IGFR3;IMD20;Low affinity immunoglobulin gamma Fc region receptor III-A
  • CD32/CD16
  • Fc gamma RII/RIII

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FCGR3A