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Fc gamma RII/CD32 Antibody (AT10) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, IP
Clone
AT10
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-65338AF350
Alexa Fluor 405
NB100-65338AF405
Alexa Fluor 488
NB100-65338AF488
Alexa Fluor 532
NB100-65338AF532
Alexa Fluor 594
NB100-65338AF594
Alexa Fluor 647
NB100-65338AF647
Alexa Fluor 700
NB100-65338AF700
Alexa Fluor 750
NB100-65338AF750
Allophycocyanin
NB100-65338APC
Allophycocyanin/Cy7
NB100-65338APCCY7
Biotin
NB100-63713
Biotin
NB100-65338B
CoraFluor 1
NB100-65338CL1
DyLight 350
NB100-65338UV
DyLight 405
NB100-65338V
DyLight 488
NB100-65338G
DyLight 550
NB100-65338R
DyLight 594
NB100-65338DL594
DyLight 650
NB100-65338C
DyLight 680
NB100-65338FR
DyLight 755
NB100-65338IR
FITC
NB100-65338F
FITC
NB100-63714
HRP
NB100-65338H
Janelia Fluor 525
NB100-65338JF525
Janelia Fluor 585
NB100-65338JF585
Janelia Fluor 635
NB100-65338JF635
Janelia Fluor 646
NB100-65338JF646
Janelia Fluor 669
NB100-65338JF669
PE
NB100-65338PE
PE/Atto594
NB100-65338PEATT594
PE/Cy5.5
NB100-65338PECY55
PE/Cy7
NB100-65338PECY7
PerCP
NB100-65338PCP
Unconjugated
NB100-65338
mFluor Violet 450 SE
NB100-65338MFV450
mFluor Violet 500 SE
NB100-65338MFV500
mFluor Violet 610 SE
NB100-65338MFV610
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Fc gamma RII/CD32 Antibody (AT10) [Janelia Fluor® 549] Summary

Immunogen
K562 cell line
Specificity
NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FCGR2B
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID:33114132).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Fc gamma RII/CD32 Antibody (AT10) [Janelia Fluor® 549]

  • CD32 antigen
  • CD32B
  • CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
  • CDw32
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc gamma RII
  • Fc gamma RIIb
  • FCG2
  • Fc-gamma RII-b
  • FcgammaRIIb
  • fc-gamma-RIIb
  • FCGR2
  • FcgRII
  • FcgRIIB
  • fcRII-b
  • FcRII-c
  • IGFR2
  • IgG Fc receptor II-b
  • low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in downmodulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FCGR2B