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Fc gamma RII/CD32 Antibody (AT10)

Images

 
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the FITC conjugate of NB100-65338. Staining of human peripheral blood granulocytes.
Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [NB100-65338] - Analysis using the Biotin conjugate of NB100-65338. Staining of human peripheral blood monocytes with mouse anti human CD32: BIOTIN.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Func, IHC, IP
Clone
AT10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

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Fc gamma RII/CD32 Antibody (AT10) Summary

Immunogen
K562 cell line
Specificity
NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FCGR2B
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 20 ug/ml
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunoprecipitation 20 ug/ml
Control
Fc gamma RIIB/CD32b Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-65338 in the following applications:

Publications
Read Publications using
NB100-65338 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID:33114132).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Fc gamma RII/CD32 Antibody (AT10)

  • CD32 antigen
  • CD32B
  • CD32Fc fragment of IgG, low affinity II, receptor for (CD32)
  • CDw32
  • Fc fragment of IgG, low affinity IIb, receptor (CD32)
  • Fc fragment of IgG, low affinity IIb, receptor for (CD32)
  • Fc gamma RII
  • Fc gamma RIIb
  • FCG2
  • Fc-gamma RII-b
  • FcgammaRIIb
  • fc-gamma-RIIb
  • FCGR2
  • FcgRII
  • FcgRIIB
  • fcRII-b
  • FcRII-c
  • IGFR2
  • IgG Fc receptor II-b
  • low affinity immunoglobulin gamma Fc region receptor II-b

Background

CD32 is a receptor for the Fc region of complexed or aggregated immunoglobulins gamma. This is a low affinity receptor. It is Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B cells. Binding to this receptor results in down modulation of previous state of cell activation triggered via antigen receptors on B cells (BCR), T cells (TCR) or via another Fc receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RII/CD32 Antibody (NB100-65338)(2)

We have publications tested in 2 confirmed species: Human, Porcine.

We have publications tested in 2 applications: FLOW, IF/IHC.


Filter By Application
FLOW
(1)
IF/IHC
(1)
All Applications
Filter By Species
Human
(1)
Porcine
(1)
All Species

Review for Fc gamma RII/CD32 Antibody (NB100-65338) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-65338:
Filter by Applications
IP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IP Human 11/16/2011
View

Summary

ApplicationImmunoprecipitation
Sample TestedHuman
SpeciesHuman

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FAQs for Fc gamma RII/CD32 Antibody (NB100-65338) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for Fc gamma RII/CD32 Antibody (NB100-65338)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Verified Customer
11/16/2011
Application: IP
Species: Human

Bioinformatics

Gene Symbol FCGR2B