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Rat Factor IX ELISA Kit (Colorimetric)

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ELISA: Rat Factor IX ELISA Kit (Colorimetric) [NBP2-82482] - Samples were spiked with high concentrations of Rat Factor IX and diluted with Reference Standard & Sample Diluent to produce samples with values within the ...read more
ELISA: Rat Factor IX ELISA Kit (Colorimetric) [NBP2-82482] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

Order Details

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Rat Factor IX ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Factor IX in samples. No Significant cross-reactivity or interference between Rat Factor IX and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.39
Intra-Assay
CV% < 5.52
Spike Recovery
88-106%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
F9

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Factor IX ELISA Kit (Colorimetric)

  • Christmas Factor
  • Coagulation Factor IX
  • EC 3.4.21
  • EC 3.4.21.22
  • F9
  • factor 9
  • FIX
  • HEMB
  • MGC129641
  • MGC129642
  • P19
  • Plasma Thromboplastic Component
  • Plasma thromboplastin component
  • PTC
  • THPH8

Background

Factor IX encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol F9