Human Factor IX ELISA Kit (Colorimetric) Summary
Description |
This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples. |
Standard Curve Range |
1.56 - 100 ng/mL; 0.313 - 20 mIU/mL (example only; lot dependent) |
Sensitivity |
0.4 ng/mL (example only; lot dependent) |
Assay Type |
Sandwich ELISA |
Inter-Assay |
%CV < 8.90 (example only; lot dependent) |
Intra-Assay |
%CV < 3.50 (example only; lot dependent) |
Spike Recovery |
99.00% (example only; lot dependent) |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
F9 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage is content dependent. |
Kit Components
Components
|
- Biotinylated Human Factor IX Antibody (50X) - 120 uL
- Chromogen Substrate (1X) - 80 uL
- EIA Diluent (10X) - 30 mL
- Human Factor IX microplate - 12 strips of 8 wells
- Human Factor IX Standard - 80 ng
- Negative Control - 1 vial
- Plate sealing tapes - 3 EA
- Positive Control - 1 vial
- SP Conjugate (100X) - 80 uL
- Stop Solution (1X) - 12 mL
- Wash Buffer (20X) - 2 x 30 mL
|
Alternate Names for Human Factor IX ELISA Kit (Colorimetric)
Background
Factor IX encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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