![Immunocytochemistry/Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained usingERK2 Antibody (PCRP-MAPK1-1D1)followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.](http://images.novusbio.com/fullsize/ERK2-Antibody-PCRP-MAPK1-1D1-Immunocytochemistry-Immunofluorescence-NBP3-13754-img0002.jpg "Immunocytochemistry/Immunofluorescence: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained usingERK2 Antibody (PCRP-MAPK1-1D1)followed by goat anti-mouse IgG-CF488. Membrane stained with phalloidin.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IP, MA |
| Clone | PCRP-MAPK1-1D1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14157) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Recombinant full-length human ERK2 protein (Uniprot: P28482) |
| Localization | Nucleus |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MAPK1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA: For coating, order antibody without BSA Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate) |
| Theoretical MW | 41.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
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![Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Flow cytometric analysis of PFA-fixed HeLa cells. ERK2 antibody (PCRP-MAPK1-1D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/ERK2-Antibody-PCRP-MAPK1-1D1-Flow-Cytometry-NBP3-13754-img0001.jpg "Flow Cytometry: ERK2 Antibody (PCRP-MAPK1-1D1) [NBP3-13754] - Flow cytometric analysis of PFA-fixed HeLa cells. ERK2 antibody (PCRP-MAPK1-1D1) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).")
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