EphB3 Antibody (647354) [Alexa Fluor® 350] Summary
Immunogen |
Mouse myeloma cell line NS0-derived recombinant human EphB3 Leu38-Ala550 Accession # P54753 |
Specificity |
Detects human EphB3 in direct ELISAs. In direct ELISAs, no cross-reactivity
with recombinant human (rh) EphA3, rhEphA4, EphB2, or recombinant mouse EphB3
is observed. |
Isotype |
IgG2a |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
EPHB3 |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.25-1 ug/10^6 cells
|
Application Notes |
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative |
0.09% Sodium Azide |
Concentration |
Please see the vial label for concentration. If unlisted please contact technical services. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for EphB3 Antibody (647354) [Alexa Fluor® 350]
Background
EphB3, also known as Cek10, Tyro6, Sek4, Hek2, and Mdk5, is a 110-130 kDa member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1). The 526 amino acid (aa) extracellular domain (ECD) of mature human EphB3 contains a ligand binding domain followed by a cysteine rich region and two fibronectin type III domains. The 418 aa cytoplasmic domain contains a tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (2). Within the ECD, human EphB3 shares 96% aa sequence identity with mouse and rat EphB3. Binding of EphB3 to its ligands Ephrin-B1, B2, and B3 triggers forward signaling through EphB3 as well as reverse signaling through the Ephrin (1, 3). EphB3 also interacts in cis with the receptor tyrosine kinase Ryk (4). Activation of its kinase is required for some but not all of the effects of EphB3 on cellular adhesion, motility, and morphology (5). EphB3 is widely expressed during development and in the adult; it shows a complementary tissue distribution to the Ephrin-B ligands (6-9). EphB3 function is important in vascular, nervous system, thymocyte, and palate development (6, 7, 10-12). It directs embyronic neuronal axon pathfinding, and its upregulation on local macrophages following neuronal injury promotes the growth of regenerating axons (10, 13). EphB3 inhibits colorectal carcinogenesis and invasion by preventing the migration of tumor cells out of the intestinal crypt (9, 14). EphB3 function is supported by the cooperative action of EphB2 in several of these processes (6, 10-12, 15).
- Pasquale, E.B. (2008) Cell 133:38.
- Bohme, B. et al. (1993) Oncogene 8:2857.
- Pasquale, E.B (2004) Nat. Neurosci. 7:417.
- Trivier, E. and T.S. Ganesan (2002) J. Biol. Chem. 277:23037.
- Miao, H. et al. (2005) J. Biol. Chem. 280:923.
- Adams, R.H. et al. (1999) Genes Dev. 13:295.
- Krull, C.E. et al. (1997) Curr. Biol. 7:571.
- Willson, C.A. et al. (2006) J. Mol. Histol. 37:369.
- Cortina, C. et al. (2007) Nature Genet. 39:1376.
- Birgbauer, E. et al. (2000) Development 127:1231.
- Alfaro, D. et al. (2008) Immunology 125:131.
- Risley, M. et al. (2009) Mech. Dev. 126:230.
- Liu, X. et al. (2006) J. Neurosci. 26:3087.
- Batlle, E. et al. (2005) Nature 435:1126.
- Holmberg, J. et al. (2006) Cell 125:1151.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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