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EphB3 Antibody (1B3)

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Western Blot: EphB3 Antibody (1B3) [H00002049-M01] - Analysis of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.Lane 1: EPHB3 transfected lysate(110.3 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: EphB3 Antibody (1B3) [H00002049-M01] - Analysis of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Western Blot: EphB3 Antibody (1B3) [H00002049-M01] - EPHB3 monoclonal antibody (M01), clone 1B3 Analysis of EPHB3 expression in HeLa.
ELISA: EphB3 Antibody (1B3) [H00002049-M01] - Detection limit for recombinant GST tagged EPHB3 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IP
Clone
1B3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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EphB3 Antibody (1B3) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
EPHB3 (NP_004434, 899 a.a. ~ 997 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AASLKVIASAQSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQ
Specificity
EPHB3 - EPH receptor B3
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EPHB3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Publications
Read Publications using
H00002049-M01 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EphB3 Antibody (1B3)

  • Cek10
  • EC 2.7.10
  • EC 2.7.10.1
  • EK2
  • EPH receptor B3
  • EphB3
  • EPH-like kinase 2
  • EPH-like tyrosine kinase-2
  • ephrin type-B receptor 3
  • ETK2Hek2
  • Hek2
  • human embryo kinase 2
  • Mdk5
  • Sek4
  • Tyro6
  • Tyrosine-protein kinase TYRO6

Background

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphB3 Antibody (H00002049-M01)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Human
(1)
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Showing Publications 1 - 7 of 7.
Publications using H00002049-M01 Applications Species
Yanina T, Stephen T, Madelen D et al. EphB3 interacts with initiator caspases and FHL-2 to activate dependence receptor cell death in oligodendrocytes after brain injury. Brain Commun. 2020-10-18 [PMID: 33305261]
Assis-Nascimento P, Tsenkina Y, Liebl DJ. EphB3 signaling induces cortical endothelial cell death and disrupts the blood-brain barrier after traumatic brain injury Cell Death Dis 2018-01-08 [PMID: 29311672] (WB, Human) WB Human
Schauer MC, Stoecklein NH, Theisen J et al. The simultaneous expression of both ephrin B3 receptor and E-cadherin in Barrett`s adenocarcinoma is associated with favorable clinical staging. Eur J Med Res. 2012-05-14 [PMID: 22583970]
Ogawa K, Takemoto N, Ishii M et al. Complementary expression and repulsive signaling suggest that EphB receptors and ephrin-B ligands control cell positioning in the gastric epithelium. Histochem Cell Biol. 2011-09-30 [PMID: 21959989]
Ishii M, Nakajima T, Ogawa K. Complementary expression of EphB receptors and ephrin-B ligand in the pyloric and duodenal epithelium of adult mice. Histochem Cell Biol. 2011-08-05 [PMID: 21818578]
Ronsch K, Jager M, Schopflin A et al. Class I and III HDACs and loss of active chromatin features contribute to epigenetic silencing of CDX1 and EPHB tumor suppressor genes in colorectal cancer. Epigenetics. 2011-05-01 [PMID: 21393996]
Schauer M, Janssen KP, Rimkus C et al. Microarray-based response prediction in esophageal adenocarcinoma. Clin Cancer Res;16(1):330-7. 2010-01-01 [PMID: 20028767]

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Bioinformatics

Gene Symbol EPHB3
Entrez
OMIM
Uniprot