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Eosinophil derived neurotoxin Recombinant Protein Antigen

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Summary
Reactivity HuSpecies Glossary
Applications AC

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Eosinophil derived neurotoxin Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human RNASE2.

Source: E. coli

Amino Acid Sequence: QFTWAQWFETQHINMTSQQCTNAMQV

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
RNASE2
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-91872.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Eosinophil derived neurotoxin Recombinant Protein Antigen

  • EC 3.1.27.5
  • EDNRNase 2
  • Eosinophil-derived neurotoxin
  • non-secretory ribonuclease
  • RAF3
  • Ribonuclease 2
  • Ribonuclease US
  • ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)
  • RNASE2
  • RNase2/EDN
  • RNS2
  • RNS2RNase UpI-2

Background

This is a non-secretory ribonuclease. It is a pyrimidine specific nuclease with a slight preference for U. Cytotoxin and helminthotoxin. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Eosinophil derived
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NBP1-91872PEP
Eosinophil derived neurotoxin Recombinant Protein Antigen
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Bioinformatics

Gene Symbol RNASE2