Home » eNOS » eNOS Antibodies » eNOS [p Ser1177] Antibody (1014F) [Unconjugated]

eNOS [p Ser1177] Antibody (1014F) [Unconjugated]

 (7 Publications)
 
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Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-eNOS (S1177) Monoclonal Antibody (Catalog # MAB9028) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for eNOS at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 µM Pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human ...read more
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with Pervanadate (PV), loaded at 0.2 mg/mL. A specific band was detected for eNOS at approximately 141 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
1014F
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB9028-SP
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eNOS [p Ser1177] Antibody (1014F) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Phosphopeptide containing the human eNOS S1177 site
Accession # P29474
Modification
p Ser1177
Specificity
Detects human eNOS when phosphorylated at S1177 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NOS3
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publications using
MAB9028 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for eNOS [p Ser1177] Antibody (1014F) [Unconjugated]

  • Constitutive NOS
  • EC 1.14.13.39
  • ECNOS
  • Endothelial NOS
  • endothelial
  • eNOS
  • eNOScNOS
  • nitric oxide synthase 3 (endothelial cell)
  • NOS type III
  • NOS3
  • NOSIII

Background

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is an enzyme encoded by the NOS3 gene. Endothelial NOS generates nitric oxide in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction and platelet aggregation. A constitutive calcium dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of the Golgi apparatus within cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for eNOS Antibody (MAB9028)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(3)
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Human
(3)
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Showing Publications 1 - 7 of 7.
Publications using MAB9028 Applications Species
Festa, J;Hussain, A;Al-Hareth, Z;Bailey, SJ;Singh, H;Da Boit, M; Phenolic Metabolites Protocatechuic Acid and Vanillic Acid Improve Nitric Oxide Bioavailability via the Akt-eNOS Pathway in Response to TNF-? Induced Oxidative Stress and Inflammation in Endothelial Cells Metabolites 2024-11-11 [PMID: 39590849] (Western Blot, Human) Western Blot Human
Festa J, Hussain A, Hackney A et al. Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis Food Science & Nutrition 7/1/2023 [PMID: 37457144]
Festa J, Hussain A, Hackney A et al. Elderberry extract improves molecular markers of endothelial dysfunction linked to atherosclerosis Food Science & Nutrition 2023-07-01 [PMID: 37457144]
T Suboti?ki, OM Ajti?, D ?iki?, JF Santibanez, M Toši?, VP ?oki? Nitric Oxide Synthase Dependency in Hydroxyurea Inhibition of Erythroid Progenitor Growth Genes, 2021-07-27;12(8):. 2021-07-27 [PMID: 34440315] (Western Blot, Human) Western Blot Human
Saeed Y. Aghdam, Doreswamy Kenchegowda, Neel K. Sharma, Gregory P. Holmes-Hampton, Betre Legesse, Maria Moroni et al. Dysregulated Cardiac IGF-1 Signaling and Antioxidant Response Are Associated with Radiation Sensitivity International Journal of Molecular Sciences 7/17/2020 [PMID: 32708958]
Cabou C, Honorato P, Briceno L et al. Pharmacological inhibition of the F1 -ATPase/P2Y1 pathway suppresses the effect of apolipoprotein A1 on endothelial nitric oxide synthesis and vasorelaxation Acta Physiol (Oxf) 3/1/2019 [PMID: 30821416]
Hai-Xin Qian Protective effect of eNOS overexpression against ischemia/reperfusion injury in small-for-size liver transplantation Exp Ther Med, 2016-09-30;12(5):3181-3188. 2016-09-30 [PMID: 27882135] (Western Blot, Human) Western Blot Human

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NOS3
Uniprot