![Western Blot: ELK3 Antibody [NBP2-16315] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ELK3-Antibody-Western-Blot-NBP2-16315-img0001.jpg "Western Blot: ELK3 Antibody [NBP2-16315] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 10% SDS PAGE gel, diluted at 1:1000.")
| Reactivity | Hu, Rt, Bv, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ELK3. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Rat (92%), Bovine (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | ELK3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-Fr usage reported in scientific literature (PMID: 26590320). |
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| Theoretical MW | 44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: ELK3 Antibody [NBP2-16315] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELK3 protein stained by ELK3 antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ELK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16315-img0003.jpg "Immunocytochemistry/Immunofluorescence: ELK3 Antibody [NBP2-16315] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ELK3 protein stained by ELK3 antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: ELK3 Antibody [NBP2-16315] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/ELK3-Antibody-Immunohistochemistry-Paraffin-NBP2-16315-img0002.jpg "Immunohistochemistry-Paraffin: ELK3 Antibody [NBP2-16315] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.")
![N/A IL-13 [Biotin]](https://images.novusbio.com/images2/DATA_IL13_DY413_ELISA_2010.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")