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ELAC2 Recombinant Protein Antigen

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

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ELAC2 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human ELAC2.

Source: E. coli

Amino Acid Sequence: WMERFGPDTQHLVLNENCASVHNLRSHKIQTQLNLIHPDIFPLLTSFRCKKEGPTLSVPMVQGECLLKYQLRPRREWQRDAIITCNPEEFIVEALQLPNFQQSVQEYR

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
ELAC2
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-84620.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for ELAC2 Recombinant Protein Antigen

  • EC 3.1.26.11
  • elaC homolog 2 (E. coli)
  • ElaC homolog protein 2
  • ELC2
  • FLJ10530
  • FLJ36693
  • FLJ42848
  • Heredity prostate cancer protein 2
  • HPC2elaC (E. coli) homolog 2
  • putative prostate cancer susceptibility protein HPC2/ELAC2
  • Ribonuclease Z 2
  • RNase Z 2
  • tRNA 3 endonuclease 2
  • tRNase Z (long form)
  • tRNase Z 2
  • zinc phosphodiesterase ELAC protein 2

Background

The protein encoded by the ELAC2 gene has a C-terminal domain with tRNA 3′ processing endoribonuclease activity,which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activatedSmad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expressioncan suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased riskof prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. (providedby RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol ELAC2