Novus Biologicals products are now on bio-techne.com

ELAC2 Overexpression Lysate

Images

 
Western Blot: ELAC2 Overexpression Lysate (Adult Normal) [NBL1-10223] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ELAC2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

ELAC2 Overexpression Lysate Summary

Description

ELAC2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC200764

Accession#: NM_018127

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
ELAC2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for ELAC2 Overexpression Lysate

  • EC 3.1.26.11
  • elaC homolog 2 (E. coli)
  • ElaC homolog protein 2
  • ELC2
  • FLJ10530
  • FLJ36693
  • FLJ42848
  • Heredity prostate cancer protein 2
  • HPC2elaC (E. coli) homolog 2
  • putative prostate cancer susceptibility protein HPC2/ELAC2
  • Ribonuclease Z 2
  • RNase Z 2
  • tRNA 3 endonuclease 2
  • tRNase Z (long form)
  • tRNase Z 2
  • zinc phosphodiesterase ELAC protein 2

Background

The protein encoded by the ELAC2 gene has a C-terminal domain with tRNA 3′ processing endoribonuclease activity,which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activatedSmad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expressioncan suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased riskof prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. (providedby RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-351
Species: Ha, Hu, Mu(-), Pm, Rt(-)
Applications: ELISA, IHC, IHC-P, KO, WB
NBP1-00092
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, PLA, WB
DKK300
Species: Hu
Applications: ELISA
NBP1-80830
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-52432
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, IHC, IHC-P, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
NBP3-04894
Species: Hu
Applications: ELISA, IHC, IHC-P, KO, WB
AF4036
Species: Hu
Applications: Simple Western, WB
NBP1-96140
Species: Hu, Mu
Applications: ChIP, Flow-IC, Flow, ICC/IF, IP, WB
NBP1-32920
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-13669
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-56603
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
H00005324-M02
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, WB
NBP2-38530
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-03005
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NB200-106
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP1-85337
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB

Publications for ELAC2 Lysate (NBL1-10223) (0)

There are no publications for ELAC2 Lysate (NBL1-10223).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ELAC2 Lysate (NBL1-10223) (0)

There are no reviews for ELAC2 Lysate (NBL1-10223). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ELAC2 Lysate (NBL1-10223) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ELAC2 Products

Research Areas for ELAC2 Lysate (NBL1-10223)

Find related products by research area.

Blogs on ELAC2

There are no specific blogs for ELAC2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ELAC2 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol ELAC2